Preparing The Lc System - Agilent Technologies InfinityLab LC Series User Manual

3 Running an Isocratic Analysis

Preparing the LC System

Preparing the LC System

1 For the isocratic pump, fill the solvent bottle with the mixture of LC- grade
2 Turn on the detector lamp and pump via the Control - System On/OFF screen.
3 For the quaternary pump, turn on the degasser by pressing the line-power
4 Purge the pump.
5 Allow the detector at least 15 min to provide a stable baseline.
6 Transfer the contents of an Agilent Technologies isocratic standard sample
7 Pump the water/acetonitrile (35/65 %) mobile phase through the column for
G4208A User's Guide
bidistilled water (35 %) and acetonitrile (65 %). For the binary or quaternary
pump, fill one solvent bottle with bidistilled water (channel A) and the other
with acetonitrile (channel B).
switch.
ampoule into a vial and seal the vial with a cap. Place the vial in position 1 of
the autosampler tray.
10 min at a flow rate of 2 mL/min.
110