Analyzing Multiple Vials Using Different Methods - Agilent Technologies InfinityLab LC Series User Manual

4 Running Multiple-Vial Analyses

Analyzing Multiple Vials Using Different Methods

Analyzing Multiple Vials Using Different Methods

This section describes how to set up a 50- vial analysis using three methods
which you have previously created called e.g. METH1, METH2 and METH3. For
example: METH1 and METH2 have the same analytical settings but differ in the
injection volume and stoptime values. METH3 uses a different temperature and
requires a wait time of 30 min for the LC system to equilibrate.
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•
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The vials are located in positions 1 to 50 of the autosampler tray.
This way of combining methods is called Sequence.
NOTE
The example assumes that the sequence table is empty. If the sequence table is
not empty, use the Delete button to delete all lines or use File - New.
1 Select the Sequence screen.
2 Move to line 1 and press Insert.
3 Select Method and then move to METH1 and press Load.
4 Move to the End of sequence list, select the Wizard button and enter:
Vial Range
#Inj.
5 Select Done to accept entries.
6 Move to the End of sequence list and select Insert.
7 Select Method and then move to METH2 and press Load.
8 Move to the End of sequence list, select the Wizard and enter:
Vial Range
#Inj.
9 Select Done to accept the entries.
G4208A User's Guide
The first 20 vials are analyzed using METH1 with one injection per vial,
the next 20 vials are analyzed using METH2 with two injections per vial,
the last 10 vials are analyzed using METH3 with three injections per vial.
1 – 20
1
21 – 40
2
118