Using The Fluorescent Unit (Ma2-Fl); Inserting/Removing The Fl Filter From The Optical Path - Nikon ECLIPSE MA200 Instructions Manual

3.19

Using the Fluorescent Unit (MA2-FL)

Second click-stop position
First click-stop position
Figure 3.19-1 Fluorescent unit
Fluorescent unit
MA2-FL G
MA2-FL B
MA2-FL BV
MA2-FL V
3.19.1

Inserting/removing the fl filter from the optical path

Fluorescent unit
Figure 3.19-2 Inserting/removing the fluorescent
unit from the optical path
Chapter 3 Operation Details
Attach the fluorescent unit to the MA200 main body to
perform the epi-fl microscopy.
A fluorescent unit consists of two types of optical
components: an excitation light filter (EX filter), a
Compensation filter
barrier filter (BA filter). You can use the four types of
attachment
combination, "G", "B", "BV" or "V" as shown in the
Excitation table below. Select the fluorescent unit suitable for
light filter
your purpose and sample.
Barrier filter
Characteristics of EX filter (nm)
510-560
450-490
400-440
380-420
Push in the unit attached on the microscope. When it
hits the second click-stop position, the fl filter enters
the optical path.
Pull out the unit. When it hits the first click-stop
position, the fl filter is removed from the optical path.
Under this condition, the compensation filter attached
to the fluorescent unit enters the optical path to
reduce glare in the bright-field observation. (Refer to
4.15.1.)
50
To perform epi-fl microscopy using the
C-HGFI/C-HGFIE HG Precentered Fiber
Illuminator as the external light source, screw the
compensation filter supplied with the fiber
adapter into the filter attachment of the
fluorescent unit. (Refer to 4.15.1.)
Characteristics of BA filter (nm)
590
520
470
450