Nikon ECLIPSE MA200 Instructions Manual page 34

Microscopy Revolving
nosepiece
• For EPI objectives
BF 5 revo.,
microscopy 6 revo., with 5 revo., LU
under the 7 revo. nosepiece adapter
epi M32-25 required
illumination
• For 6 or 7 revo., BD
objective unavailable
DF 5 revo. only BD objective only
microscopy
under the
epi
illumination
• For EPI objectives
Simplified 5 revo.,
polarization 6 revo., with 5 revo., LU
microscopy 7 revo. nosepiece adapter
under the M32-25 required
epi
• For 6 or 7 revo., BD
illumination
objective unavailable
DIC 5 revo., LU EPI objectives or LU
microscopy 6 revo. BD objective only
under the
• For EPI objectives
epi
with 5 revo., LU
illumination
nosepiece adapter
M32-25 required
• BD objective
unavailable for 6
revo.
Epi-fl 5 revo., LU EPI objectives or LU
microscopy 6 revo., BD objective only
7 revo.
• For EPI objectives
with 5 revo., LU
nosepiece adapter
M32-25 required
• For 6 or 7 revo., BD
objective unavailable
• For EPI objectives
BF 5 revo.,
microscopy 6 revo., with 5 revo., LU
under the 7 revo. nosepiece adapter
dia M32-25 required
illumination
• For 6 or 7 revo., BD
objective unavailable
• For EPI objectives
Simplified 5 revo.,
polarization 6 revo., with 5 revo., LU
microscopy 7 revo. nosepiece adapter
under the M32-25 required
dia
• For 6 or 7 revo., BD
illumination
objective unavailable
Chapter 3 Operation Details
Table 3.3-1 Selecting the microscopy method
Objective Items required for the optical
path
• MA2-PA/MA2-UPA
polarizer/analyzer unit
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
For 5 revo.:
• MA2-PA polarizer/analyzer
unit
• L-DIHC/L-DIC slider (single
NR method)
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
For 6 revo.:
• MA2-UPA polarizer/analyzer
unit
• LV-DIHC/LV-DIC slider
(Senarmont method)
• For the sensitive color
polarization microscopy,
MA2-λP λ plate available
• MA2-FL fluorescent unit
• T-P2 polarizer
• MA2-PA/MA2-UPA
polarizer/analyzer unit (for 6
revo., D-DA analyzer is also
available)
• For the sensitive color
polarization microscopy,
TI-DIC λ plate available (for 6
revo., D-LP λ plate is also
available)
BD changeover
lever
BF (Press)
⎯
DF (Pull)
⎯
BF (Press)
BF (Press)
BF (Press)
BF (Press)
⎯
BF (Press)
32
Remarks
Normal bright-field microscopy under the
episcopic illumination.
Pull out BD field changeover lever to perform
dark-field microscopy under the episcopic
illumination.
Pull out BD field changeover lever to fully
auto-open field/aperture diaphragm (The dial
position remains the same).
Pull out BD field changeover lever to exclude
polarizer/analyzer unit or fluorescent unit when
it is in the optical path.
Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol's
position.
Push λ plate into polarizer/analyzer unit and
place it into optical path.
Set bright-field microscopy under the episcopic
illumination. And then, attach
polarizer/analyzer unit to make crossed Nicol's
position. Place DIC prism into optical path to
perform DIC microscopy.
To adjust the contrast, turn the prism setting
dial for the single NR method, whereas turn the
polarizer rotation ring for Senarmont method.
Push λ plate into polarizer/analyzer unit and
place it into optical path.
Place a desired fluorescent unit G, B, BV or V
into optical path to perform epi-fl microscopy.
Normal bright-field microscopy under the
diascopic illumination.
Set bright-field microscopy under diascopic
illumination. And then, attach polarizer to
diascopic pillar illuminator and insert analyzer
into MA200 main body to make crossed Nicol's
position. (For polarizer/analyzer unit, the
analyzer part is used.)
Attach TI-DIC λ plate to supporting pillar for
dia-Illuminator 100W. For 6 revo., D-LP λ plate
is also available.