Nikon ECLIPSE MA200 Instructions Manual page 102

Problem
The brightness is insufficient.
(Refer to the troubleshooting for
the electric system too.)
Diascopic microscopy
The objective hits the sample
when switched from low to high
magnification. The sample goes
out of focus when switching
objectives.
The sample does not move
smoothly.
When viewing through the
binocular eyepiece, the image
does not resolve into a single
image.
Eye strain develops while viewing.
The coarse focus ring is heavy in
rotation.
The revolving nosepiece falls on
its own weight and the image goes
out of focus.
No interference color is seen in
the DIC microscopy.
Chapter 5 Troubleshooting
Cause
The lamp voltage is too low.
An ND filter is placed in the optical path.
The aperture diaphragm is stopped down too
far.
A polarizer, analyzer or the fluorescent unit is
placed in the optical path although the
bright-field microscopy is to be performed.
A halogen lamp is used for a dark sample.
The used objective is not suitable for the
microscopy.
The room is too bright. (for the dark-field
microscopy or the epi-fl microscopy)
The condenser position is too low.
The eyepiece diopters are not adjusted.
The eyepieces are not attached correctly.
The sample holder is not secured correctly on
the stage.
The interpupillary distance is not adjusted.
The eyepiece diopters are not adjusted.
The interpupillary distance is not adjusted.
The eyepiece diopters are not adjusted.
The brightness is not appropriate.
Eyepieces with different view-field numbers
are used for the left and right eyes.
The coarse torque adjustment ring is tightened
too much.
The coarse torque adjustment ring is loosened
too much.
No polarizer or analyzer is placed in the
optical path.
The analyzer and the polarizer are not at the
crossed Nicol's position.
No DIC prism is placed in the optical path.
The 1/4 λ plate is not attached to the polarizer.
(For Senarmont method)
The combination of the objective and the DIC
prism is wrong.
100
Countermeasure
Adjust the brightness with the brightness
control dial.
Remove the ND filter from the optical path.
Open the diaphragm to a suitable size.
Remove the polarizer, the analyzer, or the
fluorescent unit from the optical path.
Replace the light source to the brighter one.
Use the designated objective.
Darken the room.
Adjust the condenser focus knob so that the
field diaphragm image is focused on the
sample surface.
Adjust the diopters.
Mount the eyepieces correctly by aligning the
positioning grooves.
Secure the sample holder correctly.
Adjust the interpupillary distance.
Adjust the diopters.
Adjust the interpupillary distance.
Adjust the diopters.
Adjust the brightness with the brightness
control dial or ND filters.
Use eyepieces with the same view-field
number.
Loosen the coarse torque adjustment ring
adequately.
Tighten the coarse torque adjustment ring
adequately.
Place it into the optical path.
Adjust the orientation of the polarizer to make
the crossed Nicol's position.
Place it into the optical path.
Place the polarizer equipped with a 1/4 λ plate
into the optical path.
Place an appropriate objective into the optical
path.
(p.30)
(p.40)
(p.42)
(p.43, 50)
(p.56)
(p.32)
(p.59)
(p.35)
(p.81)
(p.80)
(p.34)
(p.35)
(p.34)
(p.35)
(p.30, 40)
(p.36)
(p.36)
(p.43)
(p.44)
(p.46, 47)
(p.43)
(p.46)