Nikon ECLIPSE MA200 Instructions Manual page 101

Problem
Dirt or dust is seen in the
view-field.
Diascopic microscopy
Viewing is poor (too much/little
contrast or poor resolution).
Epi-fl microscopy
Diascopic microscopy
The focus is uneven.
The image is elongated; the image
shifts during focus.
Diascopic microscopy
The image is tinged yellow.
The image is too bright.
Chapter 5 Troubleshooting
Cause
The aperture diaphragm is stopped down too
far.
Dirt or dust exists on the intermediate
magnification unit.
Dirt or dust exists on the lens, eyepiece, filter
or sample.
Dirt or dust exists on the scale slider or grain
scale slider.
The upper surface of the condenser is not
clean.
The condenser position is too low.
Dirt or dust exists on the lens, eyepiece, filter,
or sample.
The current objective is not suitable for the
microscopy.
Dirt exists on the lens for putting the eyepiece
tube or eyepiece tube entrance lens.
The aperture diaphragm is stopped down too
far.
The current fluorescent unit is not suited for
the sample.
The condenser position is too low.
The revolving nosepiece is not attached
correctly.
The revolving nosepiece is not placed to a
click-stop position. (The objective is not placed
in the optical path).
The sample holder is slanted.
The revolving nosepiece is not attached
correctly.
The revolving nosepiece is not placed to a
click-stop position.
The stage is tilting.
The microscope is not installed on a flat
surface.
The condenser has not been centered.
The NCB11 filter is not used.
The lamp voltage is too low.
The lamp voltage is too high.
99
Countermeasure
Open the diaphragm to a suitable size.
Clean the components.
Clean the components.
Clean the components.
Clean the components.
Adjust the condenser focus knob to enable the
field diaphragm image focus on the sample
surface.
Clean the components.
Use a suitable objective.
Clean the components.
Open the diaphragm to a suitable size.
Use a fluorescent unit suited for the sample.
Adjust the condenser focus knob so that the
field diaphragm image is focused on the
sample surface.
Install the revolving nosepiece correctly.
Turn the revolving nosepiece to the click-stop
position.
Attach the sample holder correctly.
Install the revolving nosepiece correctly. (p.78)
Turn the revolving nosepiece to the click-stop
position.
Attach the stage correctly.
Install the microscope on a flat and level
surface.
Center the condenser.
Place the NCB 11 filter into the optical path.
Increase the brightness with the brightness
control dial, and adjust the brightness with ND
filters.
Adjust the brightness with the brightness
control dial; place an ND filter into the optical
path.
(p.42)
(p.102)
(p.102)
(p.102)
(p.102)
(p.59)
(p.102)
(p.32)
(p.102)
(p.42)
(p.50)
(p.59)
(p.78)
(p.39)
(p.80)
(p.39)
(p.80)
(p.58)
(p.40)
(p.30, 40)
(p.30, 40)