Differential Interference Contrast Microscopy Under The Episcopic Illumination - Nikon ECLIPSE MA200 Instructions Manual

2.4

Differential Interference Contrast Microscopy under the Episcopic Illumination

Attach the items required for the differential interference contrast (DIC) microscopy
under the episcopic illumination to the microscope. (See Table 2.1.)
Focus on the sample with the bright-field microscopy under the episcopic illumination.
(See Pages 16 and 17.)
Set the microscope for the DIC microscopy under the episcopic illumination.
3-7
3-6
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Sensitive color microscopy
You can perform the sensitive color DIC microscopy using the λ plate placed into the polarizer/analyzer unit.
If the λ plate is placed into the optical path in the crossed Nicol's position (on dark background), the
background is set to sensitive red-violet. This improves the contrast of the image to the highest degree. If you
turn the polarizer rotation ring to pale-blue for background change when the λ plate is inserted, the
interference image will resemble the dark contrast image in the phase contrast microscopy. Select the
background color to achieve the desired contrast, if the phase contrast ratio varies intensively (for the rough,
uneven surface of sample).
Chapter 2 Microscopy
3-5
3-1
3-4
3-3, 5
3-2
1 Turn the revolving nosepiece to place the
objective of a desired magnification into the optical
path. (See 3.9.2.)
2 Push in the polarizer/analyzer unit to the second
click-stop position and place it into the optical
path. (See 3.13.1.)
3 Turn the polarizer rotation ring to the crossed
Nicol's position (See 3.13.2.)
4 Attach the DIC slider to the slot on the revolving
nosepiece to place the DIC prism into the optical
path. (See 3.15.1 or 3.16.2.)
5 Set the interference color. (See 3.15.3 or 3.16.3.)
For using the L-DIHC/L-DIC slider, turn the prism
movement knob, whereas for using the
LV-DIHC/LV-DIC slider, turn the polarizer rotation
ring of the polarizer/analyzer unit. In either case,
the interference color changes continuously from
dark color, gray to sensitive red-violet.
6 Turn the brightness control dial to adjust the
brightness of the episcopic illumination. (See
3.2.1.)
7 Adjust the brightness with the desired ND filter in
the filter turret. (See 3.10.)
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