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Summary of Contents for Nikon ECLIPSE TE2000-E
- Page 1 M314E 04.7.CF.3(1/5) Inverted Microscope ECLIPSE TE2000-E ECLIPSE TE2000-U ECLIPSE TE2000-S Instructions...
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Page 3: Warning / Caution Symbols Used In This Manual
Thank you for purchasing the Nikon products. This instruction manual is written for the users of the Nikon’s inverted microscopes “ECLIPSE TE2000-E, ECLIPSE TE2000-U, ECLIPSE TE2000-S” and describes the basic operations of the microscope. To ensure correct usage, read this manual carefully before operating the instrument. - Page 4 Make sure that the input voltage indication on the rear panel matches your regional voltage supply. If the voltages do not match, do not use the microscope; instead, notify your nearest Nikon representative. Using the power supply with the wrong input voltage may cause a short circuit or fire. It may also cause damage to the microscope.
- Page 5 Then use a dry cloth to wipe away the moisture. If any liquid gets inside the instrument, do not use it; instead, notify your nearest Nikon representative. Weak electromagnetic waves.
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Page 6: Notes On Handling The Microscope
Disposal of the microscope To avoid biohazard risk, dispose of the microscope as contaminated equipment according to the standard procedure of your laboratory. Notes on handling the microscope Handle the microscope gently. This product is a precision optical instrument. Handle it carefully, and do not subject it to strong shocks. The precision of the objectives in particular can be adversely affected even by weak shocks. -
Page 7: Table Of Contents
Contents Warning / Caution symbols used in this manual........... 1 Meaning of symbols used on the equipment ............1 ......................2 ......................3 Notes on handling the microscope ................ 4 Parts of the microscope and their names ............ 6 Eyepiece tubes and eyepieces..............7 Microscope base.................. -
Page 8: Parts Of The Microscope And Their Names
I. Parts of the microscope and their names I. Parts of the microscope and their names Parts of the microscope can be selected according to your purpose. Exceptionally, the combination of the lamp, dia-illuminator and the power supply is fixed. Never use these parts in combination other than specified. -
Page 9: Eyepiece Tubes And Eyepieces
I. Parts of the microscope and their names 1. Eyepiece tubes and eyepieces The following eyepiece tubes can be mounted on the observation port of the microscope. T-TD eyepiece tube D Diopter adjustment ring Eyepiece Eyepiece tube turret Open Bertrand lens (with focusing screw) Close (Shutter) 2.5X magnifier T-TS eyepiece tube S... -
Page 10: Microscope Base
I. Parts of the microscope and their names 2. Microscope base Note: Be sure to cover unused ports with supplied caps to prevent the entry of external light and dust. TE2000-E, right side Eyepiece-tube clamp screw T-HUBC HUB controller The specified AC adapter is required. Observation port Install the eyepiece tube. - Page 11 I. Parts of the microscope and their names Note: Be sure to cover unused ports with supplied caps to prevent the entry of external light and dust. TE2000-U, right side Eyepiece-tube clamp screw Observation port Install the eyepiece tube. Adapter clamp screw Front port Right-side port Photo-mask dial...
- Page 12 I. Parts of the microscope and their names Note: Be sure to cover unused ports with provided caps to prevent the entry of external light and dust. TE2000-S, right side Eyepiece-tube clamp screw Observation port Install the eyepiece tube. Optical path switch-over dial EYE: Observation 100% SIDE: Observation 20%, Left 80% Fine focus knob...
- Page 13 I. Parts of the microscope and their names Various adapters for the front port (TE2000-S is not equipped with front port.) T-BFA F-mount adapter Digital still camera with F-mount such as D1 can be mounted. T-BSLR SLR camera adapter Single-lens reflex camera such as FE10, F70, F90 and F5 can be mounted. T-BDCA direct C-mount adapter Digital still camera for the microscopes such as DXM1200, can be mounted here.
- Page 14 I. Parts of the microscope and their names T-EFN Focus Knob (supplied with the TE2000-E only. The knob is incompatible with the TE2000-U and TE2000-S.) The TE2000-E provides focus adjustment in various positions with the supplied T-EFN focus knob. You can make fine focus adjustments by connecting the T-EFN focus knob to the T- HUBC HUB controller AUX connector and by switching the knob selector switch on.
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Page 15: Dia-Illuminators
I. Parts of the microscope and their names 3. Dia-illuminators Dia-illuminators should be used in specified pairs with the lamp (12V100W or 6V30W). A lamphouse is needed for the T-DH dia-illuminator 100W. T-DH dia-illuminator 100W and LHS-H100P-1 12V100W lamphouse A: Field aperture diaphragm lever B: Filter sliders C: Condenser refocusing clamp (Works only when LWD condenser... -
Page 16: Condensers
I. Parts of the microscope and their names 4. Condensers System condenser When mounted on When mounted on T-DS dia-illuminator 30W T-DH dia-illuminator 100W (Only for HMC observation.) A: Condenser aperture diaphragm B: Condenser module C: Condenser module clamp screw D: Condenser lens (3 types available;... -
Page 17: Stages, Focusing Module
I. Parts of the microscope and their names 5. Stages, focusing module T-SR rectangular stage A: Stage ring B: Y-axis stage movement knob C: X-axis stage movement knob T-SP plain stage A: Stage ring... -
Page 18: Microscope Rear
I. Parts of the microscope and their names 6. Microscope rear Mount for epi-fl attachment MIC CTRL1 connector Connect the HUB controller. Mount for T-HUBC Not provided on TE2000-U HUB controller or TE2000-S MIC CTRL2 connector Connect the HUB controller. LAMP CTRL Not provided on TE2000-U connector Connect... -
Page 19: Power Supplies
I. Parts of the microscope and their names 7. Power supplies The bottom of the power supply becomes hot while it is in use. Do not obstruct the air vents on the sides of the power supply. TE2-PS100W power supply EXTERNAL switch Turning the switch ON activates the brightness adjustment dial on the microscope or the DIA-LAMP... -
Page 20: Microscopy
II. Microscopy II. Microscopy When using the system consisting of the TE2000-E and dia-illuminator 100W For the microscopy procedure, refer to the instruction manual supplied with the T-HUBC HUB controller. The T-HUBC HUB controller, which mounts on the rear of the microscope, controls the operations of all attached motorized units. - Page 21 II. Microscopy The TE2000 series is the system microscopes that can be flexibly configured to meet specific applications. There are three main body types, two types of dia-illuminators, four types of eyepiece tubes, and various other parts variations. The following section describes the basic microscopy procedure based on three standard system configurations. Refer to "III.
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Page 22: Microscope System Consisting Of Te2000-U, Dia-Illuminator 100W, System Condenser, And T-Td Eyepiece Tube D
II. Microscopy 1. Microscope system consisting of TE2000-U, dia-illuminator 100W, system condenser, and T-TD eyepiece tube D The following instructions are based on a microscope equipped with the parts listed below. TE2000-U microscope T-TD eyepiece tube D CFI 10X eyepiece T-DH dia-illuminator 100W LHS-H100P-1 12V100W lamphouse 12V100W halogen lamp... - Page 23 II. Microscopy Turn on the dia-illumination. 1) Set the “EXTERNAL switch” on the rear side of the power supply to ON. 2) Turn on the “power switch” of the power supply. (Flip the switch to .) 3) Press the “dia-illumination ON/OFF switch” on the left side of the microscope to turn on the lamp.
- Page 24 II. Microscopy 4) Turn the “photo-mask dial” on the front side of the microscope clockwise to remove the photo-mask from the optical path. 5) Adjust the distance between the eyepieces to consolidate the view fields of both eyepieces. Adjust the focus. 1) Place a specimen on the stage.
- Page 25 II. Microscopy Change the specimen. Use the “objective refocusing ring” on the microscope main body, the “tilting” of the dia-illuminator, and the “condenser refocusing clamp” to facilitate specimen replacement. After the completion of microscopic procedures 1) Turn off the “power switch” of the power supply. (Flip the switch to 2) After the lamphouse section cools, place the vinyl cover on the microscope.
- Page 26 II. Microscopy Center the annular diaphragm. 1) Set the “eyepiece tube turret” to position < B >, and turn the Bertrand lens focusing screw to bring the annular diaphragm image into focus. 2) Using a hexagon screwdriver, turn the two “annular diaphragm centering screws”...
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Page 27: Microscope System Consisting Of Te2000-S, Dia-Illuminator 30W, Slwd Condenser, And T-Ts Eyepiece Tube S
(-> If different, do not turn on the switch. Contact your nearest Nikon representative immediately.) 2) Turn on the “CTRL switch” on the rear side of the power supply. - Page 28 II. Microscopy Adjust the optical path. 1) Move the < 10X > objective into the optical path. 2) Set the “optical path switchover dial” on the right side of the microscope to position < EYE >. (-> Direct 100% of the light to the observation port.) 3) Place a specimen on the stage.
- Page 29 II. Microscopy 2-2. Phase-contrast (Ph) microscopy Key point: Slide the Ph annular diaphragm slider to move the annular diaphragm with the same Ph code as the objective into the optical path, then conduct microscopy. Note that the PhL diaphragm must be centered. Focus on the specimen using bright-field microscopy.
- Page 30 II. Microscopy Conduct observations using an objective with a different magnification value. 1) Move a different Ph objective of desired magnification into the optical path. 2) Slide the Ph annular diaphragm slider to set to the same Ph code as that of the objective placed in the optical path in step 1).
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Page 31: Microscope System Consisting Of Te2000-E, Dia-Illuminator 30W
(-> If different, do not turn on the switch. Contact your nearest Nikon representative immediately.) 2) Turn on the “CTRL switch” on the rear side of the power supply. - Page 32 II. Microscopy Adjust the lamp to ensure color fidelity. 1) Set the “brightness adjustment dial” on the left side of the microscope to the < 6V30W > indication. 2) Move the “NCB11 filter” on the dia-illuminator into the optical path. 3) Move the “ND4 filter”...
- Page 33 II. Microscopy Conduct observation. 1) Press the “OBJECTIVE” key on the remote control pad to select the desired magnification. 2) Move the ND filter on the dia-illuminator in and out of the optical path to adjust the brightness of the view field. Supplementary information If precise color fidelity is not required, brightness can be adjusted by varying the lamp voltage using the...
- Page 34 II. Microscopy 3-2. Phase-contrast (Ph) microscopy Key point: Slide the Ph annular diaphragm slider to move the annular diaphragm with the same Ph code as the objective into the optical path, then conduct microscopy. Note that the PhL diaphragm must be centered. Focus on the specimen using bright-field microscopy.
- Page 35 II. Microscopy Conduct observations using an objective with a different magnification value. 1) Press the “OBJECTIVE” key on the remote control pad to move a different Ph objective of desired magnification into the optical path. 2) Slide the Ph annular diaphragm slider to set to < the same Ph code >...
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Page 36: Photomicrography (Using A 35-Mm Camera Mounted On The Front Port)
II. Microscopy Photomicrography (using a 35-mm camera mounted on the front port) The following describes the procedure for taking pictures with a daylight-type color film using a single-lens reflex camera attached to the front port of the microscope. Regarding the operation of the camera, refer to the instruction manual supplied with the camera. - Page 37 II. Microscopy Adjust the lamp for color fidelity. 1) Set the “brightness adjustment dial” on the left side of the microscope to the < 12V100W > indication. 2) Move the “NCB11 filter” on the dia-illuminator into the optical path. 3) Move the “ND4 filter” on the dia-illuminator into the optical path.
- Page 38 II. Microscopy Adjust the brightness. 1) Move the “aperture diaphragm lever” on the condenser to adjust the image contrast, depth of focus, and resolution. 2) Check the camera exposure time. Longer than < 1/8 s > -> Acceptable < 1/8 s > or shorter -> Move the “ND filter” on the dia- illuminator in and out of the optical path to set the exposure time to longer than <...
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Page 39: Operation Of Each Part
III. Operation of each part III. Operation of each part In this chapter, operation of each part is described. • Refer to “II. Microscopy” for the procedures on microscopy. • If your microscope is not assembled yet, see “IV. Assembly” first. •... -
Page 40: Power On/Off
III. Operation of each part 1. Power ON / OFF Combination of the lamp, dia-illuminator and power supply The combination of the dia-illuminator and the power supply is fixed against the ratings of the lamp to be used (either 12V100W or 6V30W). See the chart below to check the correct combination of the lamp, dia-illuminator and the power supply. -
Page 41: Brightness Adjustment
III. Operation of each part 2. Brightness adjustment Brightness can be adjusted by ND filters (dimming filters) and/or the brightness adjustment dial. Adjustment with ND filters Filters that control light quantity are called ND filters. An ND filter with a large number allows less light to pass and therefore produces a darker image. -
Page 42: Optical Path Switching
III. Operation of each part 3. Optical path switching Use the “optical path switchover dial” to send the image to each port. (See page 51 for the type of the photomicrography equipment that each port can accept.) For TE2000-E Connect the T-RCP remote control pad or PC to the T-HUBC HUB controller for the switching of the optical path. For details, refer to the instruction manual supplied with the T-HUBC HUB controller. - Page 43 III. Operation of each part For TE2000-U Bottom port type To open the optical path to the bottom port, pull out the bottom port changeover lever provided on the left-hand side. To use this port, set the optical path switchover dial to position "3" and pull out the bottom port changeover lever. Bottom port changeover lever When the bottom port changeover lever is pulled out, the optical path is switched from the observation port to the bottom port.
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Page 44: Using Filters
III. Operation of each part 4. Using filters Any filter Set the appropriate filters on four filter sliders of the dia- illuminator. The following filters are available. Filter Description For brightness adjustment in normal microscopy and photomicrography. Reduces light quantity to 1/2. (Approx. 50% transmissivity.) For brightness adjustment in normal microscopy and photomicrography. -
Page 45: Using Condenser Aperture Diaphragm
III. Operation of each part 6. Using condenser aperture diaphragm The condenser aperture diaphragm adjusts the numerical aperture (N.A.) of the illumination system. Aperture of this diaphragm determines optical resolution, brightness, contrast, and depth of focus. Narrowing down the condenser aperture diaphragm decreases resolution and brightness, and increases contrast and depth of focus. -
Page 46: Eyepiece Tube Turret
III. Operation of each part 7. Eyepiece tube turret Position of the turret Description Empty The exit pupil of the objective can be observed. Turn the Bertrand lens focusing screw to focus on the exit pupil. Bertrand lens If manipulator is used, this lens can also be used to observe the tip of the manipulator located just above the objective. -
Page 47: Objectives
III. Operation of each part • • • • Specifications of the condenser lenses LWD condenser lens ELWD condenser lens N.A. 0.52 Working distance 30 mm 75 mm Available microscopy BF, Ph, DIC BF, Ph For BF: “A” For Ph: “PhL”, “Ph1”, “Ph2”, “Ph3”... - Page 48 Before using the oil immersion objective, be sure to immerse the space between the end of the objective and the specimen, with the supplied oil (Nikon immersion oil). Use the non-fluorescent oil (option) when you carry out fluorescent microscopy using the oil immersion objective for fluorescent microscopy.
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Page 49: Diopter Adjustment
III. Operation of each part 10. Diopter adjustment Perform diopter adjustment in the following way. The adjustment corrects dioptric difference of the left and right eyes, facilitates observation with both eyes, and reduces defocusing when changing the objectives. For TE2000-E, TE2000-U Turn the “photo-mask dial”... - Page 50 III. Operation of each part • When using the TE2000-E: The fine adjustment function of the focusing module of the TE2000-E is motorized, and the fine focus knob is provided on the right side of the main body. The distance of movement achieved by one rotation of the fine focus knob can be set to Fine, Middle, or Coarse.
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Page 51: T-Dh Dia-Illuminator 100W
III. Operation of each part • The fine focus knob is disabled at the Escape position. • If the objective is at the Escape position, turning the coarse focus knob to move the objective position more than 0.5 mm will clear the memorized position. •... -
Page 52: T-Sr Rectangular Stage
III. Operation of each part Tilting the pillar The pillar can be tilted to provide a wider operation space which will be needed when changing a large-sized specimen. Loosen the tilting clamp screw at the rear of the illuminator and hold the front part of the illuminating section to tilt the illuminator backward. -
Page 53: Photomicrography
III. Operation of each part 14. Photomicrography For details on assembly and usage of the camera and the photomicrography equipment, see the instruction manual supplied with each equipment. Even if all microscope components are adjusted properly, you may find the image vignetted in the finder of the camera. - Page 54 III. Operation of each part Photomicrography procedure and items to be checked On photomicrography, it is especially important to obtain appropriate illumination and focus the microscope properly. First adjust the microscope for usual observation. Then, check and perform the following For TE2000-E, TE2000-U: Rotate the diopter adjustment rings so that both the photo-mask and Perform diopter...
- Page 55 III. Operation of each part Photo-mask and the range to be taken on photo (Photo-mask is not equipped on TE2000-S.) Turning the photo-mask dial counter-clockwise will bring photo-mask in the optical path. The photo-mask can be used for diopter adjustment or trimming on photomicrography. For digital camera on the front port with T-BFA F-mount adapter (1X)
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Page 56: Assembly
IV. Assembly IV. Assembly • Before assembling the instrument, read the “ ”, “ ” and “Notes on handling the microscope” at the beginning of this manual and be sure to follow the warnings and cautions written therein. • To prevent electric shock and fire, turn off all the power switches of the system and unplug the power cords. •... - Page 57 If the two values do not match, do not use the power supply, and contact your nearest Nikon representative immediately. Use of a power supply with a different input voltage indication can result in electric shock, fire, malfunction, and/or other accidents.
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Page 58: Eyepiece Tube
IV. Assembly 2. Eyepiece tube 1) Loosen the eyepiece tube clamp screw at the front of the base Eyepiece using hexagonal screwdriver. 2) Remove the rubber cover. 3) Slide on the eyepiece tube from the front side till it hits the limit, and then fix it by the clamp screw. - Page 59 IV. Assembly 5. Filter slider Attach the filters to the filter sliders. (Push aside the claw on the circular mount when attaching the filter.) Insert the sliders on the dia-illuminator. 6. Stage Place the stage on the mounts at the center front of the base and the dia-illuminator. (If your stage is equipped with the movement handle, let it position at the right rear of the microscope though it can also be positioned at front left.) Fix the stage with four M5 hexagonal bolt supplied together with the stage.
- Page 60 IV. Assembly 8. Condenser When “T-DH dia-illuminator 100Ｗ Ｗ Ｗ Ｗ ” is used: Condenser clamp screw (Deep inside the hole.) (Attachable condensers: System condenser or ELWD-S condenser) Positioning pin Loosen the condenser clamp screw inside the hole at the Positioning right side of the condenser holder using hexagonal groove...
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Page 61: Power Supply
IV. Assembly Attaching the “SLWD condenser”: 1) Remove the dustproof and field diaphragm sliders from the illuminator. 2) Insert the SLWD condenser lens to the front, and Ph annular diaphragm slider to the rear opening. Attaching the “HMC condenser”: 1) Screw on the 52mm polarized filter inside the extension tube supplied together with the HMC condenser lens. - Page 62 IV. Assembly 10. Front port See page 51 for the photomicrography devices that can be attached to the front port and the adapters needed. 1) Pull off the plastic cap on the front port with your hand. 2) Insert the adapter and fix it by the clamp screw. (If the adapter has a positioning pin, match it with the positioning groove on the front port.) 3) Attach the photomicrography device to the adapter.
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Page 63: Troubleshooting
V. Troubleshooting V. Troubleshooting If the microscope does not function properly, take appropriate action as described below. If the problem is still not resolved after referring to “Troubleshooting,” please contact your nearest Nikon representative. 1. Optical Symptoms Causes Countermeasures Install the parts (nosepiece, condenser, etc.) Parts not correctly installed. - Page 64 . Troubleshooting Ｖ Symptoms Causes Countermeasures No ND filter in optical path. Put it in the optical path. View field too bright。 Lamp voltage too high. Lower the voltage with brightness adjustment dial. Condenser aperture diaphragm stopped Normally, set to 70 to 80% of the objective N.A. down too far.
- Page 65 V. Troubleshooting 3. Electrical Symptoms Causes Countermeasures Power does not turn on even Power cord not connected, or connected though the power Connect properly. improperly. switch is set to Lamp does not Lamp burnt. Replace with specified lamp. light. Lamp burnt too Non-conforming lamp used.
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Page 66: Care And Maintenance
Before putting on the vinyl cover, turn off the power switch on the microscope (flip it to the O side) and wait until the lamphouse is cool. 5. Periodical inspections (charged) Periodical inspections (expenses charged) of this microscope are recommended in order to maintain peak performance. Contact your nearest Nikon representative for details. -
Page 67: Technical Specifications
VII. Technical specifications VII. Technical specifications 1. When the microscope (TE2000-E, TE2000-U, TE2000-S) is used in combination with T-DH dia-illuminator 100W (for 12V100W lamp) Combination Microscope + T-DH dia-illuminator 100W + TE2-PS100W power supply Dimensions 568(W) × 727(D) × 703(H) mm Weight 41 Kg Objectives: CFI60... - Page 68 VII. Technical specifications 2. When the microscope (TE2000-E, TE2000-U, TE2000-S) is used in combination with the T-DS dia-illuminator 30W (for 6V30W lamp) When the supply voltage is 100 to 120 V: Microscope + T-DS dia-illuminator 30W + TE-PS30 power supply Combination When the supply voltage is 220 to 240 V: Microscope + T-DS dia-illuminator 30W + TE-PSE30 power supply...
- Page 69 VII. Technical specifications 3. Specified AC adapter for use with the HUB controller (only when using the T- HUBC HUB controller) Manufacturer PHIHONG ENTERPRISE (Taiwan) Type PSA30U-120 (N) Input rating AC 100-240 V, 0.7 A, 50/60 Hz Voltage fluctuation ±10% Output rating DC 12 V Rated output current...
- Page 70 NMB-003 du Canada. * Note that the microscope will not meet the above standards if used in combination with a motorized XY stage or shutter made by any manufacturer other than Nikon. ・ Meets EU IVD Directive (In vitro diagnostic medical device directive) requirements.
- Page 71 VII. Technical specifications Blank Page...
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Page 72: System Diagram
System diagram System diagram HMC MC1, MC2, MC3 Modules HMC Lens System Condenser Turret Phase PH-L, PH-1, Y-TF Trinocular Tube FUW Y-TT Trinocular Tube TUW Centering PH-2 Modules Telescope ELWD Lens Y-IDT Drawing Tube Eyepiece Teaching Heads Tubes LWD Lens Phase PH-L, PH-1, T-CT-E Motorized PH-2, PH-3;... - Page 73 System diagram Halogen Lamp 12V-100W Lambda Plate Halogen Socket 100W Filter 45mm; Filter 33mm; GIF, NCB11, ND16, ND2 GIF, Heat Absorbing, Heat Absorbing NCB11, ND16, ND2 T-P Polarizer SLWD Condenser Collector Lamphouse Lens Adapter Lamphouse Halogen Lamp 6V-30W Halogen Lamp 12V-100W LL Adapter T-DS...
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