Selecting Fluorescent Filters - Nikon ECLIPSE 50i Instructions Manual

Selecting Fluorescent Filters

3.17
A filter cube is comprised of the following three
optical components: an excitation filter (EX filter),
a barrier filter (BA filter), and a dichroic mirror
(DM). Select a filter cube with the appropriate
combination of optical components for the
specimen characteristics, the fluorescent pigment,
and the purpose intended. Keep in mind the
following:
• Different combinations of excitation and barrier
filters may be selected for the same excitation
method.
• Other types of excitation filters, barrier filters,
and dichroic mirrors can be purchased
separately.
• Excitation filters are exposed to strong light
during operations and tend to age rapidly.
Replace the excitation filter at frequent
intervals.
CAUTION
When using the UV2A or UV2B, be sure to remove the spacer inside by
unscrewing the ring retaining the excitation filter. These filters cannot be used
until the spacer inside the ring has been removed.
(Refer to 3.17.4 for details.)
Chapter 3 Individual Operations
3.17 Selecting Fluorescent Filters
54
Excitation
filter
Dichroic mirror (inside the cube)
Barrier filter