Princeton Instruments ProEM+ EMCCD User Manual page 93

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Setting the Software Parameters in LightField:
Note: The following procedure is based on LightField: you will need to modify it if you
are using a different application. Basic familiarity with the LightField software is
assumed. If this is not the case, you may want to review the software manual or have it
available while performing this procedure.
Set the software parameters as follows:
ProEM
Detector Temperature (Setup|Detector Temperature...): The default
temperature setting is read from the camera. When the array temperature
reaches the set temperature, the Detector Temperature dialog will report
that the temperature is LOCKED .
Experiment Setup|Main tab (Acquisition|Experiment Setup...):

Exposure Time: 100 µsec

Accumulations & Number of Images: 1
Experiment Setup|ROI tab (Acquisition|Experiment Setup...): Use this
function to define the region of interest (ROI).

Imaging Mode: Select this mode if you are running WinSpec.

Clicking on Full loads the full size of the chip into the edit boxes.
Experiment Setup|Timing tab (Acquisition|Experiment Setup...):

Timing Mode: Free Run, Single Trigger, or Multiple Trigger

Shutter Control: Normal

Safe Mode vs. Fast Mode: Safe Mode must be selected for Kinetics
mode operation.
Sensor expander: These parameters should be set automatically to the proper
values for your system.

Readout Mode: Available modes are read from the camera. Select
Kinetics.

Kinetics Window Height: Enter the number of exposed rows.
Temperature Setpoint (Sensor expander): The default temperature setting
is read from the camera. When the array temperature reaches the set
temperature, that status will be reported as Locked in the Sensor expander
and in the Status bar.
Common Acquisition Settings expander:

Exposure Time: 100 µsec

Number of Frames: 1

Exposures per Frame: 1
Region of Interest expander: Use the functions on this expander to define
the region of interest (ROI).

Clicking on Full Sensor selects the full window (without binning)
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+ System Manual Version 1.B