Setting Up A Kinetics Experiment; Figure 56. Typical Kinetics Experiment Layout - Princeton Instruments ProEM+ EMCCD User Manual

Chapter 6

Setting up a Kinetics Experiment

This procedure assumes that:
1. You have already set up your system in accordance with the instructions in the
2. You have read the previous sections of this chapter.
3. You are familiar with the application software.
4. The system is being operated in imaging mode.
5. The target is a sharp image, text, or a drawing that can be used to verify that the
6. You are only illuminating a portion of the CCD as shown in Figure 50.
7. You are using either WinX or LightField. WinX parameter setup is presented
Setting the Software Parameters in WinX:
Note: The following procedure is based on WinView/32: you will need to modify it if
you are using a different application. Basic familiarity with the WinView/32 software is
assumed. If this is not the case, you may want to review the software manual or have it
available while performing this procedure.
Set the software parameters as follows:
Advanced Topics

Figure 56. Typical Kinetics Experiment Layout

previous chapters.
camera is "seeing" and can be used to maximize focus.
first; LightField, second.
Controller/Camera tab (Setup|Hardware): These parameters should be set
automatically to the proper values for your system.

Controller type: This information is read from the camera.

Camera type: This information is read from the camera.

Shutter type: Custom (System dependent).

Readout mode: Available modes are read from the camera. Select
Kinetics.

Window Size: Enter the number of exposed rows.
Cleans tab (Setup|Hardware): Automatically set.
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