Figure 26. View Area Displaying An Image - Princeton Instruments ProEM+ EMCCD User Manual

Emccd cameras for imaging and spectroscopy
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3. Adjust the lens aperture, intensity scaling, and focus for the best image as viewed
4. After you have focused the camera, you can stop Preview mode, continue
Shutdown
1. Close LightField.
2. Turn off the camera power.
ProEM

Figure 26. View Area Displaying an Image

on the computer monitor. Some imaging tips follow:
Begin with the lens blocked off and then set the lens at the smallest possible
aperture (largest f-stop number).
Make sure there is a suitable target in front of the lens. An object with text or
graphics works best.
Check the brightest regions of the image to determine if the A/D converter is at
full-scale. A 16-bit A/D is at full scale when the brightest parts of the image
reach an intensity of 65535. Adjust the aperture to where it is just slightly
smaller (higher f-stop) than the setting where maximum brightness on any part
of the image occurs.
Set the focus adjustment of the lens for maximum sharpness in the viewed
image.
Preview mode, begin Acquire mode, wait for the CCD to reach the operating
temperature before going to Acquire mode, or you can shut down the system.
®
+ System Manual
Version 1.B

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