Princeton Instruments ProEM+ EMCCD User Manual page 50

Chapter 5
8. The next step is to focus the camera. Slowly move the camera in and out of
9. Tighten the spectrograph set screws to secure the spectrograph adapter. Then stop
IsoPlane SCT-320 Spectrograph
Because the ProEM+:1600 is mounted directly to the mounting plate on the IsoPlane, the
rotational alignment and focusing operations are different from the way that rotational
alignment and focusing are performed for an Acton Series spectrograph. The following
information assumes that you are familiar with the locations of the mounting plate,
Micrometer Compartment, and the locking set screw. If not, refer to the IsoPlane manual
supplied with the spectrograph.
The initial alignment assumes that you have already connected and turned on the camera
and spectrograph, have opened WinSpec, and identified the camera and spectrograph.
After you have aligned the optics the first time, you may want to repeat the process to
fine tune the alignment.
1. Mount an Princeton Instruments light source such as the dual HG/NeAr source in

Set up three ROIs (one at the top, middle, and bottom of the array) with 10
rows binned for each via the Experiment Setup|ROIs tab, Then change the
data display via the Display Layout dialog, so that 3d Graph is selected
(General tab), the Z Axis Endpoints (3D Layout tab) are %X = 0 and % Y
= 40, and the Save as Default check box is checked. After clicking on OK,
you should see three stacked graphs. Aligning the large cursor with a
prominent peak, rotate the detector until the peak aligns with the cursor in all
three ROIs.
Note: When aligning other accessories, such as fibers, lenses, optical fiber adapters,
first align the spectrograph to the slit. Then align the accessory without disturbing the
camera position. The procedure is identical to that used to focus the spectrograph
(i.e., do the alignment and focus operations while watching a live image).
focus. You should see the spectral line go from broad to narrow and back to
broad. Maximize the intensity level and minimize the FWHM of a selected peak
or peaks. You may want to use the Focus Helper function ( Process|Focus
Helper... ) to determine the narrowest line width: it can automatically locate
peaks and generate a report on peak characteristics during live data acquisition
(see the WinSpec/32 on-line help for more information).
Note that the way focusing is accomplished depends on the spectrograph, as
follows:

Long focal-length spectrographs such as the Acton SP-2300: The
mounting adapter includes a tube that slides inside another tube to move the
camera in or out as required to achieve optimum focus.

Short focal-length spectrographs: There is generally a focusing
mechanism on the spectrograph itself which, when adjusted, will move the
optics as required to achieve proper focus.

No focusing adjustment: If there is no focusing adjustment, either
provided by the spectrograph or by the mounting hardware, then the only
recourse will be to adjust the spectrograph's focusing mirror.
data acquisition.
front of the entrance slit of the spectrograph.
Operation
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