Princeton Instruments ProEM+ EMCCD User Manual page 62

Emccd cameras for imaging and spectroscopy
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Chapter 5
IsoPlane SCT-320 Spectrograph
Because the ProEM+:1600 is mounted directly to the mounting plate on the IsoPlane, the
rotational alignment and focusing operations are different from the way that rotational
alignment and focusing are performed for an Acton Series spectrograph. The following
information assumes that you are familiar with the locations of the mounting plate,
Micrometer Compartment, and the locking set screw. If not, refer to the IsoPlane manual
supplied with the spectrograph.
The initial alignment also assumes that you have already connected and turned on the
camera and spectrograph, have opened LightField, and have dragged the camera and
spectrograph icons into the Experiment Devices area. After you have aligned the optics
the first time, you may want to repeat the process to fine tune the alignment.
1. Mount a light source such as a Princeton Instruments Hg and Ne/Ar Dual
2. Set the spectrograph to 500 nm if using a mercury source or to 0.0 nm if using a
3. Wait until the camera locks at its default temperature.
4. Loosen the four screws at the corners of the camera mounting plate.
5. Select the Align Spectrometer function from the Experiment menu to open the
6. Click on the peak you will be monitoring for the alignment. This will display the
7. Slowly rotate the camera until the peaks align in all of the ROIs.
8. After completing the rotational alignment, click on the Stop button.
9. Re-tighten the four mounting plate screws.
10. Next, remove the cover from the Micrometer Compartment.
11. Using a 3/32" hex wrench, loosen the locking set screw.
12. Click on Run , and while continuously acquiring data, adjust the micrometer until
13. Tighten down the locking set screw.
14. Place the Micrometer Cover on the spectrograph. Replace and tighten all of the
15. Stop acquisition.
Switchable light source in front of the entrance slit.
broadband source.
Spectrometer Alignment dialog. This dialog describes the changes that
LightField will make to the current setup to assist you in performing rotational
alignment of the array to the spectrograph's optics. When you click on the Begin
button, the modifications are made and continuous live data will be displayed as
you rotate the camera.
data cursor that you can position at the top of the peak. Since the data cursor
spans the ROIs, you can use the data cursor as your vertical reference.
Alternatively, you can acquire an image, display the large data cursor, and
compare the vertical bar to the line shape on the screen. Rotate the camera until
the line shape on the screen is parallel with the vertical bar.
you maximize the intensity level and minimize the FWHM of a selected peak or
peaks. You may want to use the Peak Find function to identify peaks and display
FWHM widths.
cover screws.
Operation
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