Princeton Instruments ProEM+ EMCCD User Manual page 51

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2. Choose the grating and set the grating to 500 nm if using a mercury lamp or 0.0
3. Set the slit to 10 µm at a minimum.
4.
5. Wait until the camera temperature locks at its default temperature.
6. Turn on Focus mode.
7. Adjust the rotational alignment. First, use a 9/64" hex wrench to loosen the four
8. After completing the rotational alignment, re-tighten the four mounting plate
9. Remove the cover from the Micrometer Compartment.
10. Using a 3/32" hex wrench, loosen the locking set screw.
11. While continuously acquiring data, adjust the micrometer until you maximize the
12. Tighten down the locking set screw.
13. Place the Micrometer Cover on the spectrograph. Replace and tighten all of the
Acquiring Data
1. After you have completed alignment and focusing, you can stop running in
2. Make any required changes to your experiment setup and software parameters.
3. Begin running Acquire mode. Data will be acquired and displayed/stored based
ProEM
nm if using a broadband source. See "Setting the Spectrograph Parameters" on
page 46 for information about defining a spectrograph and moving the grating.
If necessary, adjust the Exposure Time to maintain optimum (near full-scale)
signal intensity.
screws at the corners of the camera mounting plate. To perform the alignment you
can:

Rotate the camera while watching a live display of the line. Choose a peak to
monitor during the rotational alignment. This peak will go from broad to narrow
and back to broad. Leave the camera rotation set for the narrowest achievable
peak.

Take an image, display the horizontal and vertical cursor bars, compare the
vertical bar to the line shape on the screen, and rotate the camera until the
line shape on the screen is parallel with the vertical bar.

Set up three ROIs (one at the top, middle, and bottom of the array) with 10 rows
binned for each via the Experiment Setup|ROIs tab, Then change the data
display via the Display Layout dialog, so that 3d Graph is selected (General
tab), the Z Axis Endpoints (3D Layout tab) are %X = 0 and % Y = 40, and the
Save as Default check box is checked. After clicking on OK, you should see
three stacked graphs. Aligning the large cursor with a prominent peak, rotate the
detector until the peak aligns with the cursor in all three ROIs.
screws. The next steps are required for focusing the camera.
intensity level and minimize the FWHM of a selected peak or peaks.
cover screws. Then stop acquisition.
Focus mode.
Changes might include adjusting the exposure time, setting up an entrance slit
shutter, changing the timing mode to External Sync, or lowering the temperature.
on the experiment settings.
®
+ System Manual Version 1.B