Lightfield Step-By-Step Procedure - Princeton Instruments ProEM+ EMCCD User Manual

104

LightField Step-by-Step Procedure

The following procedure uses a ProEM+:1600
how you can reduce readout time and increase the number of frames per second. Keep in
mind when using the approaches in this procedure that there is tradeoff between increased
spectral readout rate and data quality (increasing the frames per second tends to decrease
data quality).
Notes:
1. This same procedure can be used to increase the spectral readout rate for a
ProEM+:1600
2. The Readout Times and Frames Per Second (FPS) that you get may differ from those
reported in this procedure. These values are intended to show the effect of the
changes on Readout Time reduction and FPS.
Default Settings: Some of the default settings for a ProEM+:1600
Because software changes often occur, the default settings for your system may differ but
you could change the settings to match those presented here:
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Procedure:
1. Check the Readout Time (on the Readout expander) and the Frames Per
2. Make sure Frame Tracking and Time Stamping are not selected.
3. Change the Exposure Time to 10 μ s . The Readout Time and FPS remain
4. Change the Analog to Digital Conversion Quality to Electron Multiplied
5. Change the Region of Interest setting to Rows Binned (1600x1) Ctr 200. The
6. Change the Region of Interest setting to Rows Binned (1600x1) Ctr 10. The
7. Set the Shutter to Always Open and Closing Delay to 0 ms. The Readout
8. Set Vertical Shift Rate to 2 μs. The Readout Time is 816 μs and FPS is ~1210.
ProEM
(4)
.
Exposure Time: 33 ms
Quality: Low Noise
Speed: 100 kHz
Region of Interest: Full Sensor (1600x200)
Custom Sensor: Vertical Shift Rate =6 μs
Sensor Cleaning: Number of Clean Cycles =1, Clean Cycles Height =200,
Final Section Height =2, Final Section Count =5
Second (reported in the upper right corner of the Experiment workspace).
Based on the camera default settings, the Readout Time is 3.201 s and FPS is
~0.308.
approximately the same: Readout Time 3.201 s and FPS ~0.312.
and the Speed to 4 MHz . The Readout Time is 81.248 ms and FPS is ~11.2.
Readout Time is 1.648 ms and FPS is ~104.
Readout Time is 1.648 ms and FPS is ~104.
Time is 1.648 ms and FPS is ~603.
®
+ System Manual
(2)
camera and is meant to give you a sense of
Version 1.B
(2)
are listed below.