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10
View the specimen.
Rotate the stage knob to move the target. If the target is
not in focus, use the focus knob to adjust the focus.
Diascopic image in fluorescence observation
For fluorescence observations, press the dia-illumination LED ON/OFF switch to make the diascopic image
disappear.
Bright ambient lights will make it difficult to view the image. Nikon recommends keeping the room dark during
fluorescence observations.
To return to bright-field microscopy
●
Close the shutter of the epi-fluorescence attachment and block the light emitted by the mercury lamp.
●
Press the dia-illumination LED ON/OFF switch to turn on the dia-illumination LED.
●
Turn the filter cube switching knob and bring the position where a filter cube is not located into the optical
path.
11
Turn off the mercury lamp.
See your illuminator's manual for details.
12
Turn off the power.
Turn off the power switch (press to the "O" position) for the microscope. (The power LED on the front of the main
body will turn off.)
Chapter 1
Microscopy Procedures
37
Chap. 1-5
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