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Problem
Parts are attached incorrectly.
Lack of visibility around
periphery of field of view.
Illumination is uneven
Movable parts are not seated correctly.
across the field of view.
The field of view is not
visible.
A field diaphragm image is not focused on
the specimen surface.
The field diaphragm is stopped down too
far.
Lack of visibility around
periphery of field of view.
Incorrect combination of the objective with
Illumination is uneven
the condenser.
across the field of view.
In low magnification microscopy, required
The field of view is not
condenser operation was not performed.
visible.
The lens and specimen are dirty or dusty.
The specimen is upside down.
The thickness of the cover glass is
Out of focus with an
inadequate.
objective of high
magnification.
A fail-safe device for specimen damage
protection of the objective is pushed in.
The objective lens is attached incorrectly.
A focal deviation is high
when switching over
objectives.
Diopter adjustment has not been
performed.
The image is not in
The stage is attached incorrectly.
focus although the stage
is raised to the highest
The refocusing position is set lower than
position.
the focusing position.
Chapter 4
Troubleshooting
Cause
81
Measure
Confirm that parts (nosepiece, condenser, etc.)
are correctly attached.
(→Chapter 3, "2 Assembly for Bright-field
Microscopy")
Correctly set the optical path switching lever,
nosepiece, filter cube switchover turret,
condenser turret, and slider, etc. (Move the
part until it clicks.)
Make sure the condenser is focused and
centered.
(→Chapter 2, "5 Focusing and Centering the
Condenser".)
Open the field diaphragm slightly wider than
the field of view.
(→Chapter 2 "8 Adjusting the Field Diaphragm")
Adopt an appropriate combination.
(→Chapter 2 "7 Selecting a Condenser")
Perform the operation such as swinging out or
sliding the condenser.
Clean them as appropriate.
(→Chapter 5 "1.1 Cleaning Lenses")
Turn up the cover glass and attach it to the
stage.
(→Chapter 1 "1.2 Bright-field Microscopy Procedure
- 8 Place a specimen on the stage, and move the
stage to bring the target into view")
Attach a cover glass of the specified thickness
(0.17 mm).
Some objective has a stopper to keep the
pushed in state. Turn the tip of the object to
release.
If the objective does not have a stopper, do
not pull because the tip cannot be turned. In
this case, contact your nearest Nikon
representative.
Screw the objective all the way in.
(→Chapter 3, "2 Assembly for Bright-field
Microscopy - 7 Attach the objective".)
Perform diopter adjustment.
(→Chapter 2 "4 Adjusting the Diopter")
Attach it correctly.
(→Chapter 3, "2 Assembly for Bright-field
Microscopy - 2 Attach the stage".)
Check and reset the setting.
(→Chapter 2, "2.4 Refocusing".)
Chap. 4
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