Table of Contents
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Contents
Introduction ......................................................................................................................... i
Table of Chapters .............................................................................................................. iii
Safety Precautions ........................................................................................................... vii
Notes on Handling the Product .......................................................................................... x
Microscopy Procedures .......................................................................... 1
1
2
3
4
5
Individual Operations ............................................................................ 39
1
2
3
4
5
6
7
Contents
Contents of the Manual .................................................................................................... ii
Symbols Used in This Manual .......................................................................................... ii
WARNING and CAUTION Symbols ............................................................................... vii
Meaning of Symbols Used on the Product ..................................................................... vii
WARNING ............................................................................................................... viii
CAUTION .................................................................................................................. ix
Bright-field Microscopy .............................................................................................. 1
1.1
System Configuration and Controls ................................................................... 1
1.2
Bright-field Microscopy Procedure ..................................................................... 3
Phase Contrast Microscopy .................................................................................... 11
2.1
System Components and Controls ................................................................... 11
2.2
Phase Contrast Microscopy Procedure ........................................................... 13
Simple Polarizing Microscopy ................................................................................. 18
3.1
System Configuration and Controls ................................................................. 18
3.2
Simple Polarizing Microscopy Procedure ........................................................ 20
Sensitive Polarization Microscopy ........................................................................... 25
4.1
System Configuration and Controls ................................................................. 25
4.2
Sensitive Polarization Microscopy Procedure .................................................. 27
Epi-fluorescence Microscopy .................................................................................. 32
5.1
System Configuration and Controls ................................................................. 32
5.2
Epi-fluorescence Microscopy Procedure ......................................................... 34
Adjusting the Brightness of a Diascopic Image ....................................................... 39
1.1
Adjustment with the Dia-illumination Brightness Control Knob ........................ 39
1.2
Adjustment with ND Filters ............................................................................... 39
Focusing on the Specimen (Vertical Stage Movement) ........................................... 40
2.1
Focus Knob Rotation and Stage Movement .................................................... 41
2.2
Number of Focus Knob Turns and Distance of Stage Travel ........................... 41
2.3
Adjusting the Rotating Torque of the Coarse Focus Knob ............................... 42
2.4
Refocusing ....................................................................................................... 42
2.5
Position Exchange of the Fine Focus Knob ..................................................... 43
3.1
Knob Rotation and Stage Movement ............................................................... 44
3.2
Adjusting the Knob Heights .............................................................................. 44
3.3
Adjusting the Knob Rotation Torque ................................................................. 44
Adjusting the Diopter ............................................................................................... 45
Focusing and Centering the Condenser ................................................................. 46
Adjusting the Aperture Diaphragm .......................................................................... 47
6.1
Adjusting the Aperture Diaphragm Using the Condenser Scale ...................... 47
6.2
Adjusting the Aperture Diaphragm Using the Centering Telescope ................. 47
Selecting a Condenser ............................................................................................ 48
iv
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