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12
Focus and center the condenser.
(1)
Look into the eyepiece with the field diaphragm
stopped down to the minimum. Focus on the field
diaphragm image using the condenser focus knob,
then adjust the condenser centering screws to
center the diaphragm image within the field of view.
(2)
Bring the 40x objective into the optical path to check
Chap. 1-1
the focus and centering of the field diaphragm
image. Make adjustments the same way as step (1)
as necessary.
(3)
Turn the field diaphragm dial and adjust the field
diaphragm image so that its size is almost the same
as the field of view.
Chapter 1
Microscopy Procedures
(→See Chapter 2, "5 Focusing and Centering the Condenser" for details)
8
0.9
0.1
0.8
0.7
0.2
0.6
0.4
0.3
Field diaphragm
image
Eyepiece field ring
Field diaphragm
image
Eyepiece field ring
Focusing and centering the condenser
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