Phase Contrast Microscopy - Nikon Eclipse Ci-E Instruction Manual

Phase Contrast Microscopy

1.3
Problem
Poor contrast.
Chap. 4
Chapter 4
Cause
The Ph annular diaphragm of the
condenser does not match the phase plate
image of the objective.
The Ph annular diaphragm of the
condenser and the objective selected do
not match.
The phase contrast of the specimen is too
large.
The type of the Ph objective is not suitable
for phase contrast of the specimen.
Troubleshooting
Adjust so that they match.
(→Chapter 1, "2.2 Phase Contrast Microscopy
Procedure - 15 Center the Ph annular diaphragm".)
Put the Ph annular diaphragm with the same
Ph code as the objective into the optical path.
(→Chapter 1, "2.2 Phase Contrast Microscopy
Procedure - 17 Adjust the Ph annular diaphragm in
the condenser with the Ph objective to be used".)
Change the mounting agent or thickness of
the sample when preparing the specimen.
(→Chapter 2, "16 Tips for Phase Contrast
Microscopy".)
Use a Ph objective suitable for the specimen.
(→Chapter 2, "16 Tips for Phase Contrast
Microscopy".)
84
Measure