Epi-Fluorescence Microscopy - Nikon Eclipse Ci-E Instruction Manual

Epi-fluorescence Microscopy

1.2
Problem
Lack of visibility around
periphery of field of view.
Illumination is uneven
The filter cube is misaligned.
across the field of view.
The field of view is not
visible.
The shutter is closed.
A fluorescent image is
not visible (when the
lamp is ON).
The selection of the filter cube is incorrect.
The ND filters of the epi-fluorescence
attachment are in the optical path.
ND filters in the HG precentered fiber
illuminator are used to suppress the
brightness too much.
A halogen light source is used for a dark
The fluorescent image is
specimen.
very dark (when the
lamp is ON).
A mercury lamp in the HG precentered
fiber illuminator has reached the end of its
product life.
A designated objective is not used at UV or
V excitation.
The room is bright.
The optical path switching lever is not set
to 100% of light distribution for the
binocular section.
The dia-illumination LED is on.
The fluorescent image
The filter cube being used is not suitable
quality is poor.
for the specimen.
The objective or cover glass is dirty.
The immersion oil is fluorescent.
The contrast of the
fluorescent image is
poor.
The slide glass is fluorescent.
Stray light is entering from the condenser.
Chapter 4
Troubleshooting
Cause
83
Measure
Push the cube in to the limit.
(→Chapter 3, "6 Assembly for Epi-fluorescence
Microscopy - ■ Attaching a filter cube".)
Open the shutter.
(→Chapter 2, "17 Tips for Epi-fluorescence
Microscopy - Protecting the specimen and
preventing it from decoloration (shutter for the
epi-fluorescence attachment)".)
Use a correct filter cube.
(→Chapter 2, "17.2 Selecting Filters".)
Remove the ND filters from the optical path as
necessary.
(→Chapter 2, "17 Tips for Epi-fluorescence
Microscopy - Adjusting the brightness of the
fluorescent image (adjusting the ND filters) - ■ND
filters in the epi-fluorescence attachment".)
Adjust the brightness.
(→Check your illuminator's manual.)
Change the light source to a mercury lamp.
Replace the lamp.
(→Check your illuminator's manual.)
Use a designated objective.
Make it darker.
Switch the lever position to 100% of light
distribution for the binocular section.
(→Chapter 2, "9 Switching the Optical Path of the
Tube")
Turn off the dia-illumination LED.
Use a filter cube suitable for the specimen.
(→Chapter 2, "17.2 Selecting Filters".)
Clean it as appropriate.
(→Chapter 5 "1.1 Cleaning Lenses")
Use the non-fluorescent immersion oil
designated by Nikon.
(→Chapter 2, "17 Tips for Epi-fluorescence
Microscopy".)
Use a non-fluorescent slide glass.
(→Chapter 2, "17 Tips for Epi-fluorescence
Microscopy".)
Lower the condenser, or remove the
condenser and attach a shielding tube.
Chap. 4