Switching Excitation Methods - Nikon Eclipse 80i Instructions Manual

(6) Stop down the aperture diaphragm. (Pull the
aperture diaphragm lever.)
(7) While observing the centering tool screen,
move the center of the aperture diaphragm
image to the center of the screen. (Using the
hexagonal screwdriver provided with the
microscope, turn the field diaphragm
centering screws.) Be sure to view the
centering tool screen squarely, from the front.
(8) After centering is completed, close the
shutter, remove the centering tool, and
reinstall the objective.

Switching Excitation Methods

17.6
Up to six filter cubes each can be mounted to the
Epi-illumination attachment and to the DIH-M.
Rotate the excitation method switchover turret on
the front of the equipment to move the desired
cube into the optical path.
The number on the front section of the turret
indicates the filter cube set into the optical path.
Individual Operations
17 Fluorescence Observation
29
Excitation method
Number of the
switchover turret
fluorescent cube set
into the optical path
Aperture diaphragm
open/close lever
Aperture diaphragm
centering screw holes