Oil Immersion - Nikon Eclipse Ci-E Instruction Manual

14

Oil Immersion

CAUTION
 Petroleum benzine and absolute alcohol are highly flammable. Be careful when handling these materials, especially
around open flames and when turning the power switch on or off.
 Follow the instructions provided by the manufacturer when using absolute alcohol.
An objective with an "Oil" marking is an oil-immersion objective.
An objective of this type is to be used with designated
non-fluorescent immersion oil applied between the sample and
the tip of the objective.
For maximum performance, an oil-immersion objective with a
numerical aperture of 1.0 or larger should be combined with an
oil-immersion achromat aplanatic condenser. The oil-immersion
condenser is used by applying a designated non-fluorescent
immersion oil between the sample and the condenser.
Phase turret condenser
The phase turret condenser is intended to be used dry. Do not apply immersion oil between the condenser and the
specimen.
Use as little oil as possible (just enough to fill the space between the tip of the objective and the sample, or between the tip of
the condenser and the sample). Too much oil will result in excess oil flowing onto the stage and around the condenser.
Air bubbles in the oil
Any bubbles in the immersion oil will degrade image quality. Be careful to prevent bubbles from forming. To check for air
bubbles, fully open the field diaphragm and aperture diaphragm, remove the eyepiece, and examine the exit pupil
(bright round section) of the objective inside the eyepiece tube. If it is difficult to ascertain the presence of bubbles,
attach a centering telescope, then look for air bubbles while turning the eyepiece section of the centering telescope to
adjust the focus.
If you detect bubbles, remove them by one of the following methods:
● Turn the nosepiece slightly to move the oil-immersed objective back and forth once or twice.
In the case of the condenser, gently turn the condenser focus knob to move the condenser up and down slightly.
● Add more oil.
● Remove the oil and apply new oil.
Cleaning the oil
Oil remaining on the oil-immersion objective or adhered to the dry-type objective will significantly degrade image
quality. After use, thoroughly wipe off all oil, and make sure that no oil remains on the tips of other objectives.
Additionally, carefully wipe off the oil from the condenser.
Use petroleum benzine to wipe off the immersion oil. For optimum results, Nikon recommends cleaning with absolute
alcohol (ethyl or methyl alcohol) after cleaning with petroleum benzine.
If petroleum benzine is unavailable, use methyl alcohol alone. When using just methyl alcohol, note that surfaces will
need to be wiped repeatedly to ensure complete removal of the immersion oil. (Usually, three or four times should be
sufficient to clean the lens.)
Chapter 2
Individual Operations
57
0.1
0.2
0.5
0.6
0.7
0.9 0.8
Oil immersion
Chap. 2