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19
Adjust the field diaphragm.
Turn the field diaphragm dial to adjust the field diaphragm
so that it almost circumscribes the field of view.
Size of the field diaphragm
Normally, adjust the field diaphragm so that it
almost circumscribes the field of view. Opening
the field diaphragm too much results in an external
stray light entering the field of view, generating
flare and reducing image contrast. In addition, the
specimen will become decolorized over a wider
area.
Adjustment timing for the field
diaphragm
Be sure to adjust the field diaphragm each time
you change the objective.
20
View the specimen.
Rotate the stage knob to move the target. If the target is not in focus, use the focus knob to adjust the focus.
To switch to bright-field microscopy
● Turn the condenser turret until the [A: empty] symbol comes to the front.
● Microscopy is possible with an objective of 4x or greater, but UW microscopy with the 4x objective will
cause vignetting.
● When the condenser is set to [A: empty], its performance is equivalent to that of the Abbe condenser.
21
Turn off the power.
Turn off the power switch (press to the "O" position) for the microscope. (The power LED on the front of the main
body will turn off.)
Chapter 1
Microscopy Procedures
17
Field
diaphragm
dial
Field diaphragm
Circumscribe around the field of view
Adjusting the field diaphragm
Field of view
Chap. 1-2
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