Nikon Eclipse Ci-E Instruction Manual page 28

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Bring an arbitrary Ph objective into the optical path.
Press the nosepiece forward/reverse rotation buttons on the main body, or the nosepiece address button on the
remote control pad to move the desired Ph objective into the optical path.
When using an oil immersion type objective, apply immersion oil between the specimen and the objective.
(→Chapter 2 "14 Oil Immersion" for details)
Phase turret condenser
The phase turret condenser is intended to be used dry. DO NOT apply immersion oil between the condenser
tip's ball and the specimen.
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Adjust the Ph annular diaphragm in the condenser with the Ph objective to be used.
Turn the condenser turret to bring an annular diaphragm
Chap. 1-2
with the same Ph code as the objective into the optical
path.
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Focus on the specimen.
(1) Look into the eyepiece, and adjust the brightness of the field of view by turning the dia-illumination brightness
control knob.
(2) Rotate the stage knob to move the stage and bring the target into the optical path.
(3) If the specimen is not in focus, turn the focus knob to focus on it.
Chapter 1
Ph code
One of the Ph codes, [Ph1], [Ph2], or [Ph3] is indicated on the Ph objective depending on the size of the phase
plate. (Ph codes have nothing to do with the magnification of the objective.) Always use a Ph objective and Ph
annular diaphragm with the same Ph code. You cannot expect the phase effect if a different combination of the
codes is used.
Centering of the annular diaphragm and the phase plate
The position of each annular diaphragm in the condenser turret has already been adjusted based on the Ph1
annular diaphragm, but the phase image will differ slightly depending on how the annular diaphragm overlaps
the phase plate. For a stricter observation or capturing of still images, check whether the annular diaphragm
and the phase plate are concentric at each magnification.
Microscopy Procedures
Same Ph code
Matching the Ph codes of Ph annular
diaphragm and the Ph objective
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