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Problem
The image quality is
poor.
The contrast or the
resolution is poor.
The field of view is too
bright.
The field of view is too
dark.
Chap. 4
Color of the image
visible to the naked eye
is different from that of
the image displayed on
the monitor.
The entire field of view is
bluish or yellowish.
Chapter 4
Cause
The lens and specimen are dirty or dusty.
An aperture diaphragm is stopped down
too far. Otherwise, it is open too much.
The field diaphragm image is not focused
on the specimen surface.
(A condenser adjustment is incorrect.)
No immersion oil is applied to the tip of an
oil-immersion objective.
The designated immersion oil is not used.
The immersion oil contains air bubbles.
The immersion oil adheres to the tip of the
dry-type objective.
The brightness of the dia-illumination LED
is not adjusted appropriately.
The initial brightness setting of the
dia-illumination LED is not appropriate.
The brightness of the dia-illumination LED
is not adjusted appropriately.
The aperture diaphragm is stopped down
too far.
A field diaphragm image is not focused on
the specimen surface.
The optical path is not switched to
binocular 100%.
The initial brightness setting of the
dia-illumination LED is not appropriate.
White balance of the camera is not set
correctly.
Filter cube is in the optical path when non
epi-fluorescence observation.
Troubleshooting
Check and clean them in accordance with "Dirt
or dust does not rotate when the eyepiece is
turned" of "A dirty or dusty field of view when
looking into the eyepiece."
Open it to the proper size.
(→Chapter 2 "6 Adjusting the Aperture Diaphragm")
Make sure the condenser is focused and
centered.
(→Chapter 2, "5 Focusing and Centering the
Condenser".)
Apply our designated non-fluorescent
immersion oil.
(→Chapter 2 "14 Oil Immersion")
Remove the air bubbles.
(→Chapter 2 "14 Oil Immersion")
Clean it as appropriate.
(→Chapter 2 "14 Oil Immersion")
Adjust the brightness using the brightness control
knob to attain a suitable brightness.
(→Chapter 2, "1 Adjusting the Brightness of a
Diascopic Image")
Change the initial brightness.
(→Chapter 2, "13.2 Changing the Initial Brightness
of Dia-illumination LED".)
Adjust the brightness using the brightness control
knob to attain a suitable brightness.
(→Chapter 2, "1 Adjusting the Brightness of a
Diascopic Image")
This should normally be adjusted to 70 to 80%
of numerical aperture of the objective.
(→Chapter 2 "6 Adjusting the Aperture Diaphragm")
Make sure the condenser is focused and
centered.
(→Chapter 2, "5 Focusing and Centering the
Condenser".)
Set to binocular 100%.
(→Chapter 2, "9 Switching the Optical Path of the
Tube")
Change the initial brightness.
(→Chapter 2, "13.2 Changing the Initial Brightness
of Dia-illumination LED".)
Set the white balance in accordance with the
camera's instruction manual.
Remove the filter cube from the optical path.
80
Measure
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