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16
Adjust the orientation of the analyzer and the polarizer.
(1)
Fully open the aperture diaphragm.
(2)
Pull out one eyepiece from the tube.
(3)
Rotate the upper portion of the polarizer unit to remove the lambda plate from the optical path. When doing
this, support the polarizer unit for first-order red compensation with hands so that it does not move.
(4)
Look into the eyepiece sleeve and rotate the whole polarizer unit until you can identify a dark cross.
(You will see black stripes that change shape as you rotate the polarizer unit.)
(5)
Tighten the fixing screw of the polarizer unit to fix the polarizer.
(6)
Put the eyepiece back to the tube and set the lambda plate back into the optical path.
(7)
Rotate the lambda plate rotation lever to the left/right
limit to make sure that the field of view is colored
magenta on both sides.
17
Bring a desired objective into the optical path.
Press the nosepiece forward/reverse rotation buttons on the main body, or the nosepiece address button on the
Chap. 1-4
remote control pad to move the desired objective into the optical path.
18
Focus on the specimen.
(1)
Look into the eyepiece, and adjust the brightness of the field of view by turning the dia-illumination brightness
control knob.
(2)
Rotate the stage knob to move the stage and bring the target into the optical path.
(3)
If the specimen is not in focus, turn the focus knob to focus on it.
19
Adjust the field diaphragm.
Turn the field diaphragm dial to adjust the field diaphragm
so that it almost circumscribes the field of view.
Chapter 1
Dark cross (crossed Nicols)
You will see a dark cross when the orientation of
the analyzer orthogonally crosses that of the
polarizer.
Size of the field diaphragm
Normally, adjust the field diaphragm so that it
almost circumscribes the field of view. Opening
the field diaphragm too much results in an external
stray light entering the field of view, generating
flare and reducing image contrast. In addition, the
sample will become decolorized over a wider area.
Adjustment timing for the field
diaphragm
Be sure to adjust the field diaphragm each time
you change the objective.
Microscopy Procedures
Field
diaphragm
dial
Field diaphragm
Circumscribe around the field of view
Adjusting the field diaphragm
30
Dark cross
Field of view
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