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6
Assembly for Epi-fluorescence Microscopy
Follow the procedure described in Section 2 "Assembly for Bright-field Microscopy" to perform assembly.
Note the following:
(For details on attaching a D-FL Epi-fluorescence attachment, refer to the instruction manual provided with the D-FL
Epi-fluorescence attachment.)
■
Attaching the epi-fluorescence attachment
Attach the CI-FL Epi-fluorescence attachment to the microscope arm prior to the step 4, "Attach the tube."
(1)
Place the epi-fluorescence attachment on the
microscope arm.
(2)
Secure it by tightening the clamp screw on the arm and
two fixing bolts at the rear of the epi-fluorescence
attachment. Use a hex wrench provided with the
epi-fluorescence attachment to tighten the fixing bolts.
(3)
Attach the HG adapter of the mercury lamp illuminator
to be used to the bayonet mount on the back. (For
detailed information, refer to the instruction manual
provided with the mercury lamp illuminator.)
(4)
Place the tube on the epi-fluorescence attachment and
tighten the tube clamp screws of the epi-fluorescence
attachment using the tool provided with the microscope
to secure the tube.
■
Attaching a filter cube
WARNING
Always turn off the mercury lamp before inserting or removing a filter cube.
(1)
Remove the filter cube replacement cover on the left of
the epi-fluorescence attachment.
(2)
Insert a filter cube.
The filter cubes listed below cannot be inserted directly
into the filter bay of the epi-fluorescence attachment.
Remove an internal spacer or reverse the spacer
before insertion.
<Filter cubes requiring spacer removal>
UV-2A
UV-2B
<Filter cubes requiring spacer reversal>
DAPI
FITC
GFP-L
GFP-B
TRITC
Tx-Red
Chapter 3
Assembly
Tube clamp screw
(use a tool)
Epi-fluores-
cence
attachment
Attaching the epi-fluorescence attachment
Filter cube
replacement cover
75
Epi-fl attachment clamp bolts
(use a tool)
CUBE
1
1
2
3
4
Filter cube
UV -2A
EX 330-3
DM 400
80
BA 420
Attaching a filter cube
HG adapter
Epi-fluorescence
attachment
clamp screw
Chap. 3
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