Table of Contents
Advertisement
20
View the specimen.
(1)
Swing out the lambda plate from the optical path.(The field of view gets darker.)
(2)
Rotate the stage knob to move the target. If the target is not in focus, use the focus knob to adjust the focus.
(The specimen looks brighter in the dark field of view.)
(3)
Bring the lambda plate back into the optical path.(The background of the field of view will be colored magenta.)
(4)
Of the needle-like crystals seen in the field of view, check the color of the longitudinal ones.
(5)
Turn the lambda plate rotation lever from right to left (clockwise) to check the change of color of the crystal
being observed.
Identify the crystal by its change of color.(See the table below.)
Crystal
Urate crystal
Calcium
pyrophosphoric
acid crystal
Keep the lambda plate clean
Note that dirt such as dust and fingerprint on the lambda plate can significantly degrade the polarization
performance. Keep it clean.
To switch to bright-field microscopy
Pull out the analyzer IN/OUT knob to remove the analyzer from the optical path. Remove the polarizer unit
from the field lens.
21
Turn off the power.
Turn off the power switch (press to the "O" position) for the microscope. (The power LED on the front of the main
body will turn off.)
Chapter 1
Microscopy Procedures
Position of the lambda plate rotation lever
Leftmost
Vibration
Crystal
direction of
(yellow)
the polarizer
Vibration
Z'
direction of
Direction of the
the analyzer
lambda plate
Vibration
Crystal
direction of
(blue)
the polarizer
Vibration
Z'
direction of
the analyzer
Direction of the
lambda plate
31
Rightmost
Vibration
Crystal
direction of
(blue)
the polarizer
Vibration
direction of
Z'
the analyzer
Direction of the
lambda plate
Vibration
Crystal
direction of
(yellow)
the polarizer
Vibration
direction of
Z'
the analyzer
Direction of the
lambda plate
Chap. 1-4
Advertisement
Table of Contents
