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Problem
One side of the field of
view (up, down, right, or
left) is not focused.
The image flows (i.e.
becomes asymmetrically
defocused when moving
the focal point)
Images in left and right
eyepieces are not
coincident.
Eyes become fatigued.
The specimen does not
move smoothly.
Chap. 4
Chapter 4
Cause
The specimen is tilted relative to the stage
surface.
The stage is tilted.
Interpupillary adjustment has not been
performed.
Diopter adjustment has not been
performed.
Diopter adjustment has not been
performed.
Brightness is inadequate.
The specimen holder is not securely-fixed
to the stage.
Rotating torque of the stage knob is set too
heavy.
Troubleshooting
Position the specimen in place on the stage.
(→Chapter 1 "1.2 Bright-field Microscopy Procedure
- 8 Place a specimen on the stage, and move the
stage to bring the target into view")
Attach the stage correctly.
(→Chapter 3, "2 Assembly for Bright-field
Microscopy - 2 Attach the stage".)
Perform interpupillary adjustment.
(→Chapter 1, "1.2 Bright-field Microscopy
Procedure - 11 Adjust the interpupillary distance".)
Perform diopter adjustment.
(→Chapter 2 "4 Adjusting the Diopter")
Perform diopter adjustment.
(→Chapter 2 "4 Adjusting the Diopter")
Adjust the brightness using the brightness control
knob to attain a suitable brightness.
(→Chapter 2, "1 Adjusting the Brightness of a
Diascopic Image")
Fix the holder securely.
(→Chapter 1 "1.2 Bright-field Microscopy Procedure
- 8 Place a specimen on the stage, and move the
stage to bring the target into view")
Adjust to the appropriate torque weight.
(→Chapter 2, "3.3 Adjusting the Knob Rotation
Torque".)
82
Measure
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