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ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page
Figure 8.77 ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page
To display the
button on the
range 0.1 to 3.0 kb through a cushion of 5.7 M CsCl at 25°C in a swinging bucket rotor, separating the
RNA from chromosomal DNA contaminant. In the simulation, the sample is suspended in 2.91 M
CsCl containing 4M GuSCN layered over the CsCl cushion. See
information.
In addition to the Header Bar and the Footer Bar, the ESP RNA Pelleting in CsCl with GuSCN,
Optimized for Purity page contains the following elements:
• The
Rotor
rotor and labware to use for the simulation and select
• The
Labware
• The Supercoiled DNA button, with the --kb designation, determines molecular length. Select
the button to enter the molecular length and select
• When you have entered the fields, select the
simulation is graphically displayed.
— The blue curve represents the relative concentration of the RNA.
— The green curve represents the relative concentration of the DNA contaminant.
— The red curve represents the density of the CsCl gradient.
— The dotted line at the top of the graph represents the concentration at which CsCl
precipitates.
The maximum time required for the separation is shown above the slide bar. Use the slide bar
to display separations at shorter run times. The y-axis represents both the relative
PN B08115AF
ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity
Simulations
Page. Use this page to simulate the pelleting of RNA molecules in the
button displays the
Select Rotor and Labware Page
button also displays the
ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page
Using Simulations
(Catalog/Library). Select the
to return to this page.
OK
Select Rotor and Labware Page
to return to this page.
OK
button to display the simulated run. The
Simulate
Functional Pages
page, select the
Best Run
for more
(Catalog/Library).
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