Beckman Coulter Optima XPN User Manual page 96

Calculations
Using Calculations
Figure 6.5 Determine Sedimentation Coefficient From Run Data Page
2
Select
Select Rotor and Labware
3
Select
Material Location
meniscus, then select
4
Select
Gradient
Density gradient centrifugation is a technique used to separate proteins of different sizes from
a sample. These are the options:
• Water
• Sucrose 5% - 20%
• Sucrose 10% - 40%
• Sucrose (Custom)
Use the Sucrose (Custom) option to manually select the gradient of your choice. Once the
(Custom) option is selected, use the up or down arrow to set the limits, then select
5
Select
Particle Density
6-6
to select a rotor and labware combination, then select
to enter the material location as the percentage down the tube from the
.
OK
to select the gradient, then select
to enter the value for the particle density in g/mL, then select
.
OK
.
OK
.
OK
.
OK
PN B08115AF