Related Manuals for Beckman Coulter Optima XE Series
Summary of Contents for Beckman Coulter Optima XE Series
- Page 1 User Guide Optima XE Series Preparative Ultracentrifuges For non-In Vitro Diagnostic Use PN B07711AA May 2011 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
- Page 2 Beckman Coulter Ireland, Inc. Mervue Business Park, Mervue Galway, Ireland 353 91 774068 Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda Estr dos Romeiros, 220 - Galpao G3 - Km 38.5 06501-001 - Sao Paulo - SP - Brasil CNPJ: 42.160.812/0001-44...
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Page 3: Table Of Contents
Contents CHAPTER 1: Safety and Notices, 1-1 Alerts for Danger, Warning, Caution, Important, and Note, 1-1 Safety Notices, 1-2 Information, 1-2 Installation, 1-2 Replacement Parts, 1-3 Service, 1-3 Power Supply, 1-3 Fuse Replacement, 1-3 Mechanical Safety, 1-4 Chemical and Biological Safety, 1-4 Precautions with Liquids, 1-5... - Page 4 Contents CHAPTER 2: Description, 2-1 For non-In Vitro Diagnostic Use, 2-1 Touch Screen, 2-1 Name Rating Plate, 2-1 Rotor Chamber, 2-2 Vacuum System, 2-2 Temperature Sensing and Control, 2-2 Drive, 2-2 Safety Features, 2-3 Door, 2-3 Barrier Ring, 2-3 Imbalance Detector, 2-3 Overspeed System, 2-3...
- Page 5 Contents Step 2: Set the Speed and Rotor, 4-3 Step 3: Set the Time, 4-5 Step 4: Set the Temperature, 4-6 Step 5: Start the Run, 4-7 Zonal and Continuous Flow Operation, 4-7 Preparing for the Run, 4-8 Starting the Run, 4-9 Loading the Sample, 4-9...
- Page 6 Contents Select Language Page, 5-24 Diagnostic History Page, 5-25 Compatible Tubes for Rotor Page, 5-26 Zonal Operation Page, 5-27 Continuous Flow Operation Page, 5-28 Running Home Page, 5-29 CHAPTER 6: Maintenance and Troubleshooting, 6-1 Field Service, 6-1 Rotors and Labware, 6-1 Cleaning, 6-1 Instrument Surfaces, 6-2...
- Page 7 Contents APPENDIX C: Diagnostics, C-1 Overview, C-1 Diagnostics/User Messages Chart, C-1...
- Page 8 Contents viii...
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Page 9: Safety And Notices
CHAPTER 1 Safety and Notices This chapter presents the important notices that apply to the instrument and describes the conventions used in the document. Alerts for Danger, Warning, Caution, Important, and Note DANGER DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury. -
Page 10: Safety Notices
Beckman Coulter Representative. Installation CAUTION This instrument is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. Also, should the instrument need to be moved, a Beckman Coulter Field Service representative must reinstall and re-level the instrument in its new location. -
Page 11: Replacement Parts
Fuse Replacement WARNING Fuses protect certain electrical circuits within this instrument against overcurrent conditions. The fuse is not customer replaceable. For continued protections, please contact Beckman Coulter Field Service. PN B07711AA... -
Page 12: Mechanical Safety
Mechanical Safety DANGER For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor in use. -
Page 13: Precautions With Liquids
Certification To ensure full system quality, Beckman Coulter Optima XEs have been manufactured in a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies. -
Page 14: Cfc-Free Centrifugation
Safety and Notices CFC-Free Centrifugation CFC-Free Centrifugation To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of the Optima XE ultracentrifuge. Radio Interference This instrument is certified with regard to radio frequency interference for both the United States and Canada. -
Page 15: Software Copyright
Safety and Notices Software Copyright Software Copyright The software and other information incorporated into the Optima XE is protected by international copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws that may result in civil or criminal penalties. This computer program is also subject to additional restrictions contained in the following Microsoft OEM Customer License Agreement for Embedded Systems: “If you use the Device to access or utilize the services or functionality of Microsoft Windows XP... -
Page 16: Universal Serial Bus (Usb)
For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device. -
Page 17: Multiple Compliance
For details on proper grounding, see IEEE standard P1100. DANGER High Voltage Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative. PN B07711AA... -
Page 18: Protective Ground
Safety and Notices Summary of Instrument Labels Protective Ground This symbol is used to indicate a protective ground. This instrument must be properly grounded. Do not under any circumstances operate the instrument unless it is properly grounded. Rotor Rotation This indicates the direction of instrument rotor rotation. 1-10 PN B07711AA... -
Page 19: Symbols
Safety and Notices Symbols Symbols PN B07711AA 1-11... - Page 20 Safety and Notices Symbols 1-12 PN B07711AA...
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Page 21: Chapter 2: Description
Description The Optima XE generates centrifugal forces for the separation of particles. Classified S, it can be used with all currently manufactured Beckman Coulter preparative rotors. This chapter describes the major components of the instrument. For non-In Vitro Diagnostic Use This Optima XE centrifuge is intended for use as a general purpose laboratory instrument for the separation of components through the use of relative centrifugal force. -
Page 22: Rotor Chamber
Description Rotor Chamber Rotor Chamber The rotor chamber is made of aluminum and coated with a chemical-resistant epoxy finish. The central feature is the rotor drive spindle, but several sensors and control systems are also contained in the rotor chamber. Vacuum System The Optima XE uses a diffusion pump in series with a mechanical vacuum pump to reduce chamber pressure to a very low level. -
Page 23: Safety Features
Description Safety Features Safety Features The Optima XE ultracentrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft.). Door The high-strength structural steel chamber door has a solenoid interlock to prevent operator contact with a spinning rotor. -
Page 24: Specifications
Description Specifications Specifications Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. Control Features Specifications Description Speed • Set speed: — 1,000 to rated speed in 100-rpm increments • Speed display: — Actual measured rotor speed in 10-rpm increments below 1000 rpm and 100-rpm increments ≥1000 rpm •... -
Page 25: Physical Data
Description Specifications Specifications Description Temperature • Set temperature: — 0 to 40°C in 1°C increments • Rotor Temperature (after equilibration): — ± 0.5°C over entire set temperature range for all rotors except SW-32 — ± 0.8°C over entire set temperature range for SW-32 •... -
Page 26: Audible Sounds
Description Specifications Specification Description Finishes • Rotor pad: — Coated polycarbonate • Top and Front surfaces: — Polyurethane • Other surfaces: — Acrylic baking enamel Electrical • Power requirement: — 200–240 VAC, 30A branch circuit, 50/60 Hz — Automatic tap selection on every run •... -
Page 27: Available Rotors
• Door open during Zonal or Continuous Flow mode Available Rotors All currently manufactured Beckman Coulter floor model ultracentrifuge rotors can be used in the Optima XE ultracentrifuge. The rotors are described in individual manuals that accompany each rotor. Information on rotors and accessories is available in the Rotor Catalog accessible from the touch screen. - Page 28 Description Available Rotors PN B07711AA...
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Page 29: Chapter 3: The Touch Screen Interface
CHAPTER 3 The Touch Screen Interface This chapter describes the touch screen interface for the Optima XE. Except for the power switch, all the controls for the instrument are programmed controls that appear on the touch screen. Figure 3.1 Home Page Areas on the Screen The items on the screen may change, depending on the state of the instrument. -
Page 30: Header Bar
The Touch Screen Interface Areas on the Screen Header Bar Figure 3.2 Header Bar The area across the top of the screen is called the Header Bar, containing four important items: Page Button • Home Button • Menu Display • Status •... - Page 31 The Touch Screen Interface Areas on the Screen Status Display Figure 3.5 Ready Status The Status Display is in the middle of the Header Bar and shows you the current instrument status. The background color of the header bar changes with the type of status: •...
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Page 32: Footer Bar
The Touch Screen Interface Areas on the Screen Help Button Figure 3.11 Help Button Help button gives you access to the built-in online help messages. Help messages are described at the end of this chapter. Footer Bar The bottom of the touch screen always shows the footer bar. The footer bar appears in two different ways, but it always has the Button at the left and the Button at the right. -
Page 33: Footer Bar On The Home Page
The Touch Screen Interface Areas on the Screen Footer Bar on the Home Page On the Page, the middle of the Footer Bar shows three Items: Home • The Display/Button Vacuum • The System Name • The Display/ Display/Button Accel Decel Figure 3.14 Footer Bar - Home Page Each of these is explained below. -
Page 34: Accel And Decel Display/Buttons
The Touch Screen Interface Help Messages Accel and Decel Display/Buttons These are two displays that, together, act as a single button. As a display, each shows the selected acceleration or deceleration profile currently selected. If you select either one of them, it takes you to the Set Acceleration/Deceleration Profiles Page, described in the next chapter. -
Page 35: Global Help
The Touch Screen Interface Help Messages Global Help Button appears next to the Button. If you select the Button, the Global Help Help Global Help Page Display Area shows you a description of the current page and all the elements it contains. Figure 3.17 Global Help Button attached to Help Button While using Global Help, you also have some additional options: •... - Page 36 The Touch Screen Interface Help Messages Figure 3.20 Home Page Help with opaque screen Select the Button again to return to the ghost screen image. Transparency • If you need information about some other aspect of the instrument, you can select the Table of Contents link at the bottom of the page to see the outline of all the available help pages.
- Page 37 The Touch Screen Interface Help Messages • If the global help page is too large to fit in the page display area, a Scroll Bar appears on the right side, allowing you to scroll up and down through the page. Figure 3.22 Zonal/Continuous Flow Operation Help with scroll bar PN B07711AA...
- Page 38 The Touch Screen Interface Help Messages 3-10 PN B07711AA...
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Page 39: Chapter 4: Operations
CHAPTER 4 Operations The touch screen interface for the Optima XE makes operations about as simple as possible. Although there are many additional things you can do, this chapter outlines basic operations: • Manual Operation • Operations with Preconditioning • Continuous Flow Operation •... -
Page 40: Step 1: Start On The Home Page
Operations Manual Operation Start the run. Step 1: Start on the Home Page Start at the Page. If your screen shows any other page, press the Button in the Home Home Page upper left corner of the screen. Figure 4.1 Home Page NOTE If your system is in Zonal Mode or Continuous Flow Mode, you have to press the Button to go... -
Page 41: Step 2: Set The Speed And Rotor
Operations Manual Operation Figure 4.2 Zonal Operation Page with Button Cancel Step 2: Set the Speed and Rotor Press the Display/Button on the Page to go to the Page. Set Speed Home Set Speed Figure 4.3 Set Speed Page PN B07711AA... - Page 42 Operations Manual Operation When the Page appears, follow these steps: Set Speed Press the Button to go to the Page. Select Rotor Select Rotor and Labware Figure 4.4 Select Rotor and Labware Page Select the rotor and labware for the run from the list. Select the Button to return to the Page.
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Page 43: Step 3: Set The Time
Operations Manual Operation Step 3: Set the Time Press the Display/Button to go to the Page. Set Time Set Time Figure 4.5 Set Time Page On the Page, follow two steps: Set Time Use the keypad to set the desired time in hours and minutes. Use the keys Backspace Clear... -
Page 44: Step 4: Set The Temperature
Operations Manual Operation Step 4: Set the Temperature Press the Display/Button to go to the Page. Set Temp Set Temperature Figure 4.6 Set Temperature Page On the Page, follow two steps: Set Temperature Use the keypad to set the desired temperature in degrees Celsius. Use the Backspace Clear keys to make corrections. -
Page 45: Step 5: Start The Run
Operations Zonal and Continuous Flow Operation Step 5: Start the Run Once the run values are set, start the run: Prepare your samples and place them in the rotor following all proper procedures, including balanced weight distribution. Preheat or precool the rotor and samples, if necessary. Mount the rotor in the instrument following all the procedures in the rotor manual and observing all safety procedures and cautions. -
Page 46: Preparing For The Run
Operations Zonal and Continuous Flow Operation or continuous flow operations have been authorized, the Page or the Zonal Operation Continuous Page replaces the Page. The mode does not end until the run is complete or Flow Operation Home the user selects the Button to end the mode. -
Page 47: Starting The Run
Operations Zonal and Continuous Flow Operation For Continuous Flow operation, you may have two different load speed settings. The initial setting is for alignment and adjustment checks, which you set in this phase. When everything else is ready, mount the rotor and select the Display/Button to begin Vacuum preconditioning the chamber. -
Page 48: Running The Sample
Operations Zonal and Continuous Flow Operation Running the Sample The instrument highlights Running on the Status Display, accelerates to the set run speed, and begins counting down the set run time. The run phase ends in one of three ways: •... -
Page 49: Functional Pages
CHAPTER 5 Functional Pages This chapter describes the functional pages that you use to control the Optima XE Ultracentrifuge. They include all of the following: Home Page • Menu Page • Set Speed Page • Set Time Page • Set Speed Time Page (w²t Mode) •... -
Page 50: Home Page
Functional Pages Home Page Home Page Page is the first operational page to appear after the instrument startup. You return to it Home when you select the Page Button on the Header Bar. It is the basic page from which you begin Home most operations. -
Page 51: Set Speed Display/Button
Functional Pages Home Page Set Speed Display/Button Figure 5.3 Set Speed Display/Button Display/Button shows the current rotor speed as a large number and also shows the Set Speed current run speed setting in small characters across the bottom. Display/Button takes you to the Set Speed Page (or to the Set Speed Time Page (w²t... -
Page 52: Menu Page
Functional Pages Menu Page Menu Page Figure 5.6 Menu Page Page appears when you select the Button in the Header Bar, allowing you access to Menu Menu the menu choices. Choosing an option dismisses the page and goes to the selected page. Menu •... -
Page 53: Set Speed Page
Functional Pages Set Speed Page Set Speed Page Figure 5.7 Set Speed Page (No Rotor Selected) Figure 5.8 Set Speed Page (Rotor Selected) Page appears when you select the Display/Button on the Page or the Set Speed Set Speed Home Footer Bar, allowing you to set the speed for the next run. -
Page 54: Set Time Page
Functional Pages Set Time Page • The Button selects the type of speed units. This button is disabled until a rotor is RPM/RCF selected. • The Button takes you to the Select Rotor and Labware Page. Select Rotor • The Button discards your changes and dismisses the page. -
Page 55: Set Speed Time Page (W²T Mode)
Functional Pages Set Speed Time Page (w²t Mode) Set Speed Time Page ( ²t Mode) ω Figure 5.10 Set Speed Time Page (²t Mode) ω Page appears when the ²t mode is active and you select either the Set Speed Time Set Speed ω... -
Page 56: Set Temperature Page
Functional Pages Set Temperature Page Set Temperature Page Figure 5.11 Set Temperature Page Page appears when you select the Button on the Page Set Temperature Set Temperature Home or the Footer Bar, allowing you to set the temperature for the next run. In addition to the Header Bar and the Footer Bar, the Page contains the following Set Temperature... -
Page 57: Set Acceleration/Deceleration Profiles Page
Functional Pages Set Acceleration/Deceleration Profiles Page Set Acceleration/Deceleration Profiles Page Figure 5.12 Set Acceleration/Deceleration Profiles Page Page appears when you select the Button/ Set Acceleration/Deceleration Profiles Accel/Decel Display in the Footer Bar, allowing you to choose profiles for acceleration and deceleration. In addition to the Header Bar and the Footer Bar, the Page Set Acceleration/Deceleration Profiles... -
Page 58: Example Acceleration And Deceleration Profiles
Functional Pages Set Acceleration/Deceleration Profiles Page Example Acceleration and Deceleration Profiles The values associated with each acceleration and deceleration profile are given in the tables below. NOTE The values shown should be considered as approximations for time and rpm. Acceleration Deceleration Profile # Time... -
Page 59: Select Rotor And Labware
Functional Pages Select Rotor and Labware Select Rotor and Labware Figure 5.13 Select Rotor and Labware Page Page appears when you select the Button on either the Page Select Rotor and Labware Set Speed or the Page, allowing you to select a rotor for the next run. Set Speed Time This is different from the Page, which shows rotor specifications, but does not select... -
Page 60: References Page
Functional Pages References Page References Page Figure 5.14 References Page Page appears when you select the Button in the Page, allowing you References References Menu to choose from the available reference materials to view. In addition to the Header Bar and the Footer Bar, the Page contains the following References elements:... -
Page 61: System Options Page
Functional Pages System Options Page System Options Page Figure 5.15 System Options Page Page appears when you select the Button in the Page, allowing System Options Options Menu you to choose any of the available system option setting pages. In addition to the Header Bar and the Footer Bar, the Page contains the following System Options elements:... -
Page 62: Zonal Authorization Page
Functional Pages Zonal Authorization Page Zonal Authorization Page Figure 5.16 Zonal Authorization Page Page appears when you select the Button on the Page, Zonal Authorization Zonal Operation Menu allowing you to use Zonal mode for the next run. Page contains the In addition to the Header Bar and the Footer Bar, the Zonal Authorization following elements:... -
Page 63: Continuous Flow Authorization Page
Functional Pages Continuous Flow Authorization Page Continuous Flow Authorization Page Figure 5.17 Continuous Flow Authorization Page Page appears when you select the Continuous Flow Authorization Continuous Flow Operation Button on the Page, allowing you to use Continuous Flow mode for the next run. Menu In addition to the Header Bar and the Footer Bar, the Page contains... -
Page 64: Service Login Page
Functional Pages Service Login Page Service Login Page Figure 5.18 Service Login Page Page appears when you select the Button on the Page. Service Login Service Mode Menu This page is for service personnel only. If you accidently find yourself on the Page, Service Login select the... -
Page 65: About Page
Functional Pages About Page About Page Figure 5.19 About Page Page appears when you select the Button on the Page. This page presents About About Menu system information about your instrument. In addition to the Header Bar and the Footer Bar, the Page contains the following operating About elements:... -
Page 66: Rotor Catalog Page
Functional Pages Rotor Catalog Page Rotor Catalog Page Figure 5.20 Rotor Catalog Page Page appears when you select the Button on the Page, Rotor Catalog Rotor Catalog References allowing you to examine the detailed specifications for all the rotors available for your instrument. This is different from the Page where you choose a rotor from the Select Rotor and Labware... -
Page 67: Labware Catalog Page
Functional Pages Labware Catalog Page Labware Catalog Page Figure 5.21 Labware Catalog Page Page appears when you select the Button on the Labware Catalog Labware Catalog References Page, allowing you to examine the detailed specifications for the labware available for the rotors your instrument can use. -
Page 68: Export User Manual Page
Functional Pages Export User Manual Page Export User Manual Page Figure 5.22 Export User Manual Page Selection of the Button allows you to copy the User Manual to a USB drive. Export User Manual NOTE Do not remove the USB drive while a data transfer is in progress. In addition to the Header Bar and the Footer Bar, the Page contains the following Export User Manual... -
Page 69: Set System Name Page
Functional Pages Set System Name Page Set System Name Page Figure 5.23 Set System Name Page Page appears when you select the Button on the System Set System Name Set System Name Options Page, allowing you to set the System Name that appears in the Footer Bar on the Page. -
Page 70: Set Date And Time Page
Functional Pages Set Date and Time Page Set Date and Time Page Figure 5.24 Set Date and Time Page Page appears when you select the Button on the System Set Date and Time Set Date and Time Options Page, allowing you to set the instrument's internal time and date. In addition to the Header Bar and the Footer Bar, the Page contains the following Set Date and Time... -
Page 71: Set Sound Page
Functional Pages Set Sound Page Set Sound Page Figure 5.25 Set Sound Page Page appears when you select the Button on the System Options Page, Set Sound Set Sound allowing you to set the sound volume and enable or disable the key click option. In addition to the Header Bar and the Footer Bar, the Page contains the following Set Sound... -
Page 72: Select Language Page
Functional Pages Select Language Page Select Language Page Figure 5.26 Select Language Page Page appears when you select the Button on the System Select Language Select Language Options Page, allowing you to choose the language the instrument uses in operations. In addition to the Header Bar and the Footer Bar, the Page contains the following Select Language... -
Page 73: Diagnostic History Page
Functional Pages Diagnostic History Page Diagnostic History Page Figure 5.27 Diagnostic History Page Page appears when you select the Button on the System Diagnostic History Diagnostic History Options Page, allowing you to examine and export the details of incidents that caused a diagnostic message on the instrument (warnings and error messages). -
Page 74: Compatible Tubes For Rotor Page
Functional Pages Compatible Tubes for Rotor Page Compatible Tubes for Rotor Page Figure 5.28 Compatible Tubes for Rotor Page Page appears when you select the Button on the Compatible Tubes for Rotor Labware Rotor Page, allowing you to examine the detailed specifications for the labware for the selected Catalog rotor. -
Page 75: Zonal Operation Page
Functional Pages Zonal Operation Page Zonal Operation Page Figure 5.29 Zonal Operation Page Page appears when you enter the correct password and select the Zonal Operation Authorize Button from the Page, putting the instrument in Zonal mode. Zonal Authorization When you use Zonal mode, the Page replaces the Page until Zonal mode ends. -
Page 76: Continuous Flow Operation Page
Functional Pages Continuous Flow Operation Page Continuous Flow Operation Page Figure 5.30 Continuous Flow Operation Page Page appears when you enter the correct password and select the Continuous Flow Operation Button from the Page, putting the instrument in Authorize Continuous Flow Authorization Continuous Flow mode. -
Page 77: Running Home Page
Functional Pages Running Home Page Running Home Page Figure 5.31 Running Home Page Page appears when there is an actual run in progress. Running Home In addition to the Header Bar and the Footer Bar, the Page contains the following Running Home elements: •... - Page 78 Functional Pages Running Home Page 5-30 PN B07711AA...
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Page 79: Chapter 6: Maintenance And Troubleshooting
Reference documents for detailed instructions on their care. Cleaning The methods and materials used in the following procedures have been tested by Beckman Coulter and will not damage the instrument if used as instructed. CAUTION Before using any other materials or methods, check with Beckman Coulter to verify that they will not damage the instrument. -
Page 80: Instrument Surfaces
If the instrument and/or accessories are contaminated with radioactive or pathogenic solutions, follow appropriate decontamination procedures as determined by your laboratory safety officer. Refer to Chemical Resistances (publication IN-175), or contact Beckman Coulter Field Service to ensure that the decontamination method does not damage any part of the instrument (or accessories). -
Page 81: Sterilization And Disinfection
Sterilization and Disinfection WARNING While Beckman Coulter has tested these methods and found that they do not damage the instrument, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods. -
Page 82: Rotor Stopped At Restoration
Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Rotor Stopped at Restoration If the rotor has stopped spinning when power is restored, the instrument cancels the run and sends a diagnostic message to alert you to that the run was cancelled due to a power failure. Getting Access to the Sample If a power failure lasts for several hours, you may have to remove the sample from the rotor while there is no power to the instrument. - Page 83 Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Figure 6.1 Panel screws and Tabs 1. Panel Tabs 3. Front Panel 2. Top Panel 4. Latch Screws When all three latch screws are loose, use your hand to lift the top panel until it pops loose. Lift the front panel until it is free and tilt the top edge towards you.
- Page 84 Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure WARNING When working on the inside of the instrument, be careful not to touch any wires or circuitry. Figure 6.2 Internal Parts 1. Fan Housing 2. Vacuum Vent Port Cap 3.
- Page 85 Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Vent the Chamber Although you checked for noise and vibration, there is still a possibility that the rotor is still spinning. If it is still spinning, you can hear a whining noise when you begin to vent the chamber. If you hear the whining noise when you begin to vent the chamber, you must close the port immediately and wait for the rotor to come to a stop.
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Page 86: Restoring The Instrument To Operating Condition
The instrument is again ready for use when power is restored. Storage and Transportation To ensure that the instrument does not get damaged, contact Beckman Coulter Field Service for specific instructions and/or assistance in preparing the equipment for transport or long-term storage. -
Page 87: Supply List
Supply List Supply List Contact Beckman Coulter Sales for assistance ordering parts and supplies. Customers in the United States call 1-800-742-2345. For international contacts, see the website at www.beckmancoulter.com or use the contact numbers on the inside front cover of this book. A partial list of supplies is given below for your convenience. - Page 88 Maintenance and Troubleshooting Supply List 6-10 PN B07711AA...
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Page 89: Appendix A: Preinstallation Requirements
The following information is provided in case the instrument must be relocated. Contact Beckman Coulter Field Service to adjust and level the instrument if it must be moved. The pads on each leveling leg are designed to prevent possible rotation of the instrument in the case of a rotor mishap. -
Page 90: Ventilation
Preinstallation Requirements Space Requirements WARNING Maintain a 30-cm (1-ft.) clearance envelope around the ultracentrifuge while it is running. No persons or any hazardous materials should be within this clearance boundary while the ultracentrifuge is operating except to change operating controls, if required. Figure A.1 Safety and ventilation space Ventilation If you choose to install the instrument with less than the 12 in./30.5 cm. -
Page 91: Electrical Requirements
A.2) to connect the equipment to earth ground. In regions where the instrument is supplied with an unterminated cord, a plug that meets local electrical and safety requirements must be supplied. (Contact your local Beckman Coulter office for specific information regarding these requirements.) See Table A.1... - Page 92 Preinstallation Requirements Electrical Requirements PN B07711AA...
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Page 93: Appendix B: Special Warranty For The Optima™ Xe
Any product claimed to be defective must, if requested by Beckman Coulter be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges. - Page 94 〉 ----------------------------------- - labor and travel. NOTE For details of drive coverage with a service contract, contact your local Beckman Coulter service representative Conditions 1. The drive has been operated only within its rated speed and temperature ranges. 2. The drive unit has not been subjected to unequal loading, improper rotor installation, corrosion from material spilled onto the hub or accumulated in the chamber of the instrument.
- Page 95 CHAPTER 6, Maintenance and Troubleshooting. For any maintenance not covered in this manual, contact Beckman Coulter Field Service for assistance. USA customers can call 1-800-742-2345. For international contacts, see the website at www.beckmancoulter.com or use the contact numbers on the inside front cover of this book.
- Page 96 D113 - Touch Screen Touch Screen USB cable Be sure USB touch screen cable is connected. Not Detected not connected If problem persists, call Beckman Coulter Field Service. D114 - Code error Bad checksum on code Call Beckman Coulter Field Service.
- Page 97 Diagnostics/User Messages Chart Table C.1 Diagnostics/User Messages Chart (Continued) Message Definition/Result Recommended Action A122 - Alert: Firmware Firmware error caused Call Beckman Coulter Field Service. error reset D123 - UI Communications error Call Beckman Coulter Field Service. Communications A124 - Alert: Hard Hard drive is 90% full Backup files soon.
- Page 98 1. Make sure the rotor is installed properly. 2. Check the condition of the rotor overspeed disk. 3. Verify the rotor load is within limits specified in the rotor manual. 4. If the problem persists, call Beckman Coulter Field Service. PN B07711AA...
- Page 99 4 minutes and properly lubricated. 2. Check for sample leakage. Clean and dry the rotor chamber if needed. 3. If the problem persists, call Beckman Coulter Field Service. D402 - Slow Vacuum Vacuum not <20 microns 1. Make sure door o-ring is clean, undamaged, after 20 minutes and properly lubricated.
- Page 100 Diagnostics/User Messages Chart Table C.1 Diagnostics/User Messages Chart (Continued) Message Definition/Result Recommended Action D503 - Temperature Can thermistor not Call Beckman Coulter Field Service. Control connected D504 - Temperature Can thermistor shorted Call Beckman Coulter Field Service. Control D505 - Temperature Can temperature out of Call Beckman Coulter Field Service.
- Page 101 2. Check the condition of the rotor overspeed disk. 3. Verify the rotor load is within limits specified in the rotor manual. 4. If the problem persists, call Beckman Coulter Field Service. D607 - Drive Drive frequency error Call Beckman Coulter Field Service.
- Page 102 Diagnostics Diagnostics/User Messages Chart PN B07711AA...
- Page 104 © 2010-2011 Beckman Coulter, Inc. All Rights Reserved...
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