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3
Select
Gradient
For more information on gradients, refer to the Calculations chapter.
4
Select
Density
5
Select
°C
6
Select the buttons with the colored indicators to enter the values for Sedimentation Coefficient
of the particles, then select
Users can enter up to three sedimentation coefficients for three particles.
NOTE
A sedimentation coefficient value is usually reported as s20,w, which means at 20°C in water. The
ESP is designed to simulate the separation of particles in Sucrose with density gradients at a set
temperature. Therefore, for the simulation, enter sedimentation coefficients that correspond to the
those in Sucrose at a set temperature.
7
Select
Simulate
The green, blue, and red curves represent the sedimentation coefficients of the three particles.
Move the slider to the left to display conditions at shorter run times.
8
To simulate another run, select
9
Select
Transfer
live run. Select
can be run at a later time.
ESP Plasmid Run
This function simulates an optimized plasmid DNA separation in homogeneous 1.55 g/mL CsCl-EtBr
at 25°C, and controls rotor speed to prevent CsCl precipitation. The simulation predicts the length
of time required to achieve a stable separation of the supercoiled (intact) and linear (nicked)
plasmid DNA.
To create an ESP plasmid run simulation:
1
On the
The system displays the ESP Optimized Plasmid DNA Separation page.
PN B08115AF
to select the gradient of the Sucrose solution, then select
to enter the density in g/mL of the particles to be separated, then select
to enter the run temperature, then select
.
OK
. The simulation is displayed on the graph.
Reset
to transfer the current simulated run settings to the instrument settings for a
to save the current simulated run settings as a named run program, which
Save
page, select
Simulations
ESP Plasmid Run
.
OK
to clear all the parameters that were entered.
.
7
Simulations
Using Simulations
.
OK
.
OK
7-9
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