Esp Rna (Best); Esp Rna (Best) - Beckman Coulter Optima L-XP Instructions For Use Manual

Simulations

ESP RNA (BEST)

5
(
Press
specific filename, the program is automatically saved to a temporary file called LastPlasmid.pla.
6
(
Press

ESP RNA (BEST)

Two simulations are provided for the pelleting of RNA molecules in the range 0.1 to 3.0 kbp through
a cushion of 5.7 M CsCl at 25°C in a swinging bucket rotor. The RNA (Best) simulation, of a sample
suspended in 2.91 M CsCl containing 4 M GuSCN layered over a CsCl cushion occupying one-third of
the tube volume, is optimized for purity.
To set up an ESP RNA (Best) run:
1
Select
ESP RNA Run (Best)
2
Select the particle length (see
Figure 3.5 ESP RNA (Best) Screen
3
Select the rotor (swinging bucket only) and tube in use.
3-6
)
to transfer the run parameters to the Settings window. If not saved to a
Download
) ( )
, then to run the program.
Enter Start
from the Simulate menu.
Figure 3.5).
PN LXP-IM-7AB