Esp Optimized Plasmid Dna Separation Page; Select Rotor And Labware Page - Beckman Coulter Optima XPN User Manual

Functional Pages

ESP Optimized Plasmid DNA Separation Page

ESP Optimized Plasmid DNA Separation Page

Figure 8.81 ESP Optimized Plasmid DNA Separation Page
To display the
the Simulations
homogeneous 1.55 g/mL CsCl-EtBr at 25°C. The simulation predicts the time at which the required
separation will occur. The simulation controls the (simulated) rotor speed to ensure that the CsCl
does not reach a density at the bottom of the tube that could cause precipitation. See
Run for more information.
In addition to the Header Bar and the Footer Bar, the
contains the following elements:
• The
Rotor
rotor and labware to use for the simulation and select
• The
Labware
• The Supercoiled DNA button, with the --kbp designation, determines molecular length. Select
the button to enter the molecular length and select
• When you have entered the fields, select the
the graph above. The x-axis is the relative position (volume) of gradient in the tube. The y-axis
is the relative concentration (density) of the materials. You can move the slider below the
graph to see the effect of a shorter run time.
• The
Reset
• The
Back
• The
Transfer
a live run.
8-78
ESP Optimized Plasmid DNA Separation
Page. Use this page to simulate an optimized plasmid DNA separation in
button displays the

Select Rotor and Labware Page

button also displays the
button clears all the fields.
button returns to the Simulations
button transfers the current simulated run settings to the instrument settings for
pages, select the
ESP Optimized Plasmid DNA Separation
(Catalog/Library). Select the
to return to this page.
OK
Select Rotor and Labware Page
to return to this page.
OK
button to display the simulated run in
Simulate
Page.
button on
ESP Plasmid Run
ESP Plasmid
page
(Catalog/Library).
PN B08115AF