Page 1 User Guide Optima XPN Series Preparative Ultracentrifuges PN B08115AF February 2018 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
Page 2 © 2018 Beckman Coulter, Inc. All rights reserved Trademarks Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.
Page 3: Revision History
This document applies to the latest software listed and higher versions. When a subsequent software version affects the information in this document, a new issue will be released to the Beckman Coulter website. For labeling updates, go to www.beckmancoulter.com and download the latest version of the manual or system help for your instrument.
Page 4 Revision History PN B08115AF...
Page 5: Safety And Notices
Safety and Notices This chapter presents the important notices that apply to the instrument and describes the conventions used in the document. Alerts for Danger, Warning, Caution, Important, and Note DANGER DANGER indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury.
Page 6: Safety Notices
Beckman Coulter Representative. Installation CAUTION This instrument is designed to be installed by a Beckman Coulter Field Service representative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. Also, should the instrument need to be moved, a Beckman Coulter Field Service representative must reinstall and re-level the instrument in its new location.
Page 7: Replacement Parts
Refer such servicing to qualified personnel. NOTE It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service. Power Supply DANGER To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and plug to connect this equipment to earth-ground.
Page 8: Mechanical Safety
Mechanical Safety DANGER For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor in use.
Page 9: Precautions With Liquids
Scope of Manual This manual is designed to familiarize you with the Optima XPN, its functions, specifications, operation, and routine operator care and maintenance. Read this entire manual, especially the safety notices and all safety-related information, before operating the instrument or performing maintenance.
Page 10: Cfc-Free Centrifugation
Optima XPN ultracentrifuge. Software Copyright The software and other information incorporated into the Optima XPN is protected by international copyright laws. Unauthorized copying, use, distribution, transfer, or sale is a violation of those laws that may result in civil or criminal penalties. This computer program is also subject to additional...
Page 11: Rohs Notice
Safety and Notices RoHS Notice RoHS Notice These labels and materials declaration table (the Table of Hazardous Substance's Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements. China RoHS Caution Label This label indicates that the electronic information product contains certain toxic or hazardous substances.
Page 12: Summary Of Instrument Labels
Safety and Notices Summary of Instrument Labels Summary of Instrument Labels This section provides information for some labels and symbols appearing on the Optima XE instrument housing. These labels and symbols may be associated with user-serviceable procedures. Individual hazards associated with a specific procedure in this manual may use these labels and symbols, and are included in within the procedures for that task.
Page 13: Recycling Label
For Beckman Coulter products bearing this label, please contact your dealer or local Beckman Coulter office for details on the take-back program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal of the device.
Page 14: Caution Static Sensitive Area
Operation, replacement or servicing of any components where contact with bare, live hazardous parts could occur, possibly resulting in electric shock, should only be performed by your Beckman Coulter representative. Protective Ground This symbol is used to indicate a protective ground. This instrument must be properly grounded.
Page 15: Output Plate Label
Safety and Notices Summary of Instrument Labels Output Plate Label This is the label that indicates what the output rating are on the instrument. Rotor Rotation This indicates the direction of instrument rotor rotation. PN B08115AF...
Page 16: Symbols
Safety and Notices Symbols Symbols PN B08115AF...
Page 17: Table Of Contents
Contents Revision History, iii Safety and Notices, v Alerts for Danger, Warning, Caution, Important, and Note, v Safety Notices, vi Information, vi Installation, vi Replacement Parts, vii Service, vii Power Supply, vii Fuse Replacement, vii Mechanical Safety, viii Chemical and Biological Safety, viii Precautions with Liquids, ix...
Page 18 Contents Name Rating Plate, 1-1 Rotor Chamber, 1-1 Vacuum System, 1-1 Temperature Sensing and Control, 1-2 Drive, 1-2 Safety Features, 1-2 Door, 1-2 Barrier Ring, 1-3 Imbalance Detector, 1-3 Overspeed System, 1-3 Dynamic Rotor Inertia Check (DRIC), 1-3 Specifications, 1-3 Control Features, 1-4 Physical...
Page 19 Contents Step 4: Set the Time, 3-6 Step 5: Set the Temperature, 3-7 Step 6: Start the Run, 3-9 Zonal and Continuous Flow Operation, 3-10 Preparing for the Run, 3-11 Starting the Run, 3-11 Loading the Sample, 3-12 Running the Sample, 3-12 Unloading the Sample, 3-12...
Page 20 Contents CHAPTER 6: Calculations , 6-1 Using Calculations, 6-1 Calculations Page, 6-1 Reduce Rotor Speed For Dense Solutions, 6-3 Reduce Rotor Speed For Precipitating Solutions, 6-4 Sedimentation Coefficient From Run Data, 6-5 Sedimentation Coefficient From Molecular Mass , 6-7 Pelleting Time, 6-8 Refractive Index , 6-9...
Page 21 Contents Import Page, 8-23 Real-Time Run Data/Historical Run Data Page, 8-24 Run Graph Options Page, 8-25 Run History Page, 8-26 Run History Filter Page, 8-28 Before Run/After Run Comment Page, 8-29 Menu Page, 8-30 System Options Page, 8-31 The Basic Tab, 8-31 The System Tab, 8-32...
Page 22 Contents Chemical Resistances Page, 8-59 Calculations Page , 8-60 Reduce Rotor Speed for Dense Solutions Page, 8-61 Reduce Rotor Speed for Precipitating Solutions Page, 8-62 Determine Sedimentation Coefficient from Run Data Page, 8-63 Determine Sedimentation Coefficient from Molecular Mass Page, 8-64 Calculate Pelleting Time Page, 8-65 Calculate Concentration Measures...
Page 23 Contents Storage and Transportation, 9-10 Supply List, 9-11 Replacement Parts, 9-11 Supplies, 9-11 APPENDIX A: Preinstallation Requirements, A-1 Overview, A-1 Space Requirements, A-1 Safety, A-1 Ventilation, A-2 Temperature, A-2 Electrical Requirements, A-2 APPENDIX B: Special Warranty for the Optima XPN, B-1 Special Warranty, B-1 APPENDIX C:...
Page 24 Illustrations Illustrations Home Page, 2-1 Header Bar, 2-1 Home Page Button, 2-2 Menu Button, 2-2 Ready Status, 2-2 Header Bar - Ready Status, 2-3 Header Bar - Running Status, 2-3 Header Bar - Stopping Status, 2-3 Header Bar - Warning Message, 2-3 2.10 Header Bar - Error...
Page 25 Illustrations 3.11 Continuous Flow Page, 3-12 Run History Button, 4-10 Run History Filter, 4-11 Set From Date and Time, 4-11 History, 4-12 Run Graph Button, 4-12 Run History Button, 4-13 Run Graph Options, 4-13 Run Graph Button, 4-14 Run History Button, 4-14 4.10 Run History...
Page 26 Illustrations Simulations Page, 7-3 ESP RNA Pelleting in CsCI with GuSCN, Optimized for Purity Page, 7-4 ESP RNA Pelleting in CsCI with GuSCN, Optimized for Speed Page, 7-5 ESP Pelleting Separation Page, 7-7 ESP Rate Zonal Separation Page, 7-8 ESP Optimized Plasmid DNA Separation Page, 7-10 Substitute Rotor Run Page, 7-11...
Page 27 Illustrations 8.30 Run History Filter Page, 8-28 8.31 Before Run Comment Page, 8-29 8.32 Menu Page, 8-30 8.33 System Options Page, Basic Tab, 8-31 8.34 System Options Page, System Tab, 8-32 8.35 System Options Page, Network Tab, 8-33 8.36 System Options Page, Users Tab, 8-34 8.37 System Options Page, Reports...
Page 28 Illustrations Page, 8-64 8.68 Calculate Pelleting Time Page, 8-65 8.69 Calculate Concentration Measures Page, 8-66 8.70 Calculate Refractive Index Page, 8-67 8.71 About Page, 8-68 8.72 Zonal Authorization Page, 8-69 8.73 Zonal Mode Button, 8-69 8.74 Zonal Operation Page, 8-70 8.75 Continuous Flow Operation Page, 8-70...
Page 29: Tab
Tables Tables User Levels, 4-5 Required Wire Connections, A-3 Diagnostics/User Messages Chart, C-1 xxix...
Page 30 Tables...
Page 31: Description
CHAPTER 1 Description The Optima XPN generates centrifugal forces for the separation of particles. Classified S, it can be used with all currently manufactured Beckman Coulter rotors for floor-model preparative ultracentrifuges. This chapter describes the major components of the instrument.
Page 32: Temperature Sensing And Control
Externally cooled by forced air and internally cooled by oil, the drive delivers ultra-smooth, quiet performance, with high rotor-imbalance tolerance. Safety Features The Optima XPN ultracentrifuge has been designed and tested to operate safely indoors at altitudes up to 2000 m (6562 ft.). Door The high-strength structural steel chamber door has a solenoid interlock to prevent operator contact with a spinning rotor.
Page 33: Barrier Ring
Description Specifications Barrier Ring A 41-mm (1.63-in.) steel alloy armor ring acts as the primary barrier, surrounded by a 13-mm (0.5-in.) vacuum chamber to provide full protection for the operator. Imbalance Detector An imbalance detector monitors the rotor during the run, causing automatic shutdown if rotor loads are severely out of balance.
Page 34: Control Features
Description Specifications Control Features Specifications Description Speed • Set speed: — 1,000 to rated speed in 100-rpm increments • Maximum speed: — XPN-80: 80,000 rpm — XPN-90: 90,000 rpm — XPN-100: 100,000 rpm • Speed display: — Actual measured rotor speed in 10-rpm increments below 1000 rpm and 100-rpm increments ≥1000 rpm •...
Page 35 Description Specifications Specifications Description Temperature • Set temperature: — 0 to 40°C in 1°C increments • Rotor Temperature (after equilibration): — ± 0.5°C over entire set temperature range for all rotors except SW-32 — ± 0.8°C over entire set temperature range for SW-32 •...
Page 36: Physical Data
Description Specifications Physical Data Specification Description Dimensions • Width : — 94.0 cm (37 in.) • Depth: — 68.1 cm (26.8 in.) • Height: — 125.7 cm (49.5 in.) • Weight — 485 kg (1068 lb) • Sides Ventilation Clearances —...
Page 37: Audible Sounds
Normally, only non-conductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation must be expected. Audible Sounds The Optima XPN instrument makes an audible sound for the following events: • Boot up • Start of Run • End of Run •...
Page 38: Available Rotors
Available Rotors Available Rotors All currently manufactured Beckman Coulter floor model ultracentrifuge rotors can be used in the Optima XPN ultracentrifuge. The rotors are described in individual manuals that accompany each rotor. Information on rotors and accessories is available in the Rotor Catalog accessible from the touch screen.
Page 39: Chapter 2: The Touch Screen Interface
CHAPTER 2 The Touch Screen Interface This chapter describes the touch screen interface for the Optima XPN. Except for the power switch, all the controls for the instrument are programmed controls that appear on the touch screen. Figure 2.1 Home Page Areas on the Screen The items on the screen may change, depending on the state of the instrument.
Page 40: Menu Button
The Touch Screen Interface Areas on the Screen Display • Status Button • Help The following sections explain these items. Home Page Button Figure 2.3 Home Page Button Button displays the Home Page from any other page. When you use Zonal Mode or Home Page Continuous Flow Mode, the Page replaces the...
Page 41: Help Button
The Touch Screen Interface Areas on the Screen Figure 2.6 Header Bar - Ready Status • Green background: in operation (run in progress). Figure 2.7 Header Bar - Running Status • Green background: in operation (stop in progress). Figure 2.8 Header Bar - Stopping Status •...
Page 42: Start Button
The Touch Screen Interface Areas on the Screen Start Button Figure 2.12 Start Button Button begins a run with the current settings. Use it only after you have set the Start parameters for the run. Stop Button Figure 2.13 Stop Button button stops the current run immediately.
Page 43: Footer Bar On The Home Page
The Touch Screen Interface Areas on the Screen Footer Bar on the Home Page On the Page, the middle of the Footer Bar shows three Items: Home • The Display/Button Vacuum • The System Name • The Display/Button Accel Decel Figure 2.14 Footer Bar - Home Page Each of these is explained below.
Page 44: Accel And Decel Display/Button
The Touch Screen Interface Help Messages Accel and Decel Display/Button These are two displays that, together, act as a single button. As a display, each shows the selected acceleration or deceleration profile currently selected. Select the button to display the Set Acceleration/Deceleration Profiles Page, described in CHAPTER...
Page 45: Global Help
The Touch Screen Interface Help Messages Global Help Button appears next to the Button. If you select the Button, the Global Help Help Global Help Page Display Area shows you a description of the current page and all the elements it contains. Figure 2.17 Global Help Button attached to Help Button While using Global Help, you also have some additional options: •...
Page 46: System References
The Touch Screen Interface System References Figure 2.20 Home Page Help with opaque screen Select the Button again to return to the ghost screen image. Transparency • If you need information about some other aspect of the instrument, you can select the Table of Contents link at the bottom of the page to see the outline of all the available help pages.
Page 47: About Your Instrument
References About Your Instrument The system About page contains information about your instrument, including the model number, serial number, and software version, that you need when you call Beckman Coulter Field Service. Follow this procedure to display the About Page.
Page 48: About Page
The Touch Screen Interface About Your Instrument Figure 2.23 About Page Select the button on the header bar to display the page. Menu Menu Select the button to display the t page. About Abou 2-10 PN B08115AF...
Page 49: Chapter 3: Operations
CHAPTER 3 Operations The touch screen interface for the Optima XPN simplifies operations. Although there are many additional things you can do, this chapter outlines basic operations: • Manual Operation • Operations with Preconditioning • Continuous Flow Operation • Zonal Operation...
Page 50: Step 1: Start On The Home Page
Operations Manual Operation Start the run. Step 1: Start on the Home Page Start at the Page. If your screen shows any other page, select the Button in the Home Home Page upper left corner of the screen. Figure 3.1 Home Page NOTE If your system is in Zonal Mode or Continuous Flow Mode, you must select the Cancel Button to go to the Home Page.
Page 51: Step 2: Set The Speed And Rotor
Operations Manual Operation Figure 3.2 Zonal Operation Page with Button Cancel Step 2: Set the Speed and Rotor Select the Display/Button on the Page to go to the Page. Set Speed Home Set Speed Figure 3.3 Set Speed Page PN B08115AF...
Page 52 Operations Manual Operation When the Page appears, follow these steps: Set Speed If you need to record rotor selection, select the Button to go to the Select Rotor Select Rotor and Page. Labware Figure 3.4 Select Rotor and Labware Page Select the rotor and labware for the run from the library.
Page 53: Step 3: Set The Acceleration And Deceleration Profiles
Operations Manual Operation Step 3: Set the Acceleration and Deceleration Profiles Select the Display/Button to go to the Page. Accel and Decel Set Acceleration/Deceleration Profiles Figure 3.5 Set Acceleration/Deceleration Profiles Page On the Page, follow these steps: Set Acceleration/Deceleration Profiles Select the desired Acceleration and Deceleration profiles.
Page 54: Step 4: Set The Time
Operations Manual Operation Step 4: Set the Time Select the Display/Button to go to the Page. Set Time Set Time Figure 3.6 Set Time Page On the Page, follow these steps: Set Time Use the keypad to set the desired time in hours and minutes. Use the keys to Back Clear...
Page 55: Step 5: Set The Temperature
Operations Manual Operation Figure 3.7 Delay Start Page • Select to set a starting or stopping time. Then you can set the date and Start At Stop At time in the fields above. Select OK to return to the Page. Set Time Select the key to accept your entry and dismiss the page.
Page 56: Set Temperature Page
Operations Manual Operation Figure 3.8 Set Temperature Page On the Page, follow two steps: Set Temperature Use the keypad to set the desired temperature in degrees Celsius. Use the keys Back Clear to make corrections. Select the key to accept your entry and dismiss the page. PN B08115AF...
Page 57: Step 6: Start The Run
Operations Manual Operation Step 6: Start the Run Once the run values are set, start the run: NOTE If you have entered a delayed start, you must select the Start button to begin the countdown to the delayed start. Prepare your samples and place them in the rotor following all proper procedures, including balanced weight distribution.
Page 58: Zonal And Continuous Flow Operation
Operations Zonal and Continuous Flow Operation Zonal and Continuous Flow Operation WARNING In zonal and continuous flow operation, the operator is unavoidably exposed to rotating machinery. For safety, the operator must be properly instructed and qualified. Guard against accidentally dropping objects, such as pens, pencils, or hemostats into the chamber.
Page 59: Preparing For The Run
Operations Zonal and Continuous Flow Operation • Stopping • Finishing Each phase is outlined in a section below. Preparing for the Run The rotor manual details the preparations for a run, which may include cleaning, assembling, and lubricating the rotor, setting up pumps and tubing, and usually includes overnight cooling to precondition the rotor, sample and solutions used in the run.
Page 60: Loading The Sample
Operations Zonal and Continuous Flow Operation Figure 3.11 Continuous Flow Page When the rotor reaches the Loading speed, the status display highlights Loading, to show that you have moved into the next phase. Loading the Sample In the Loading phase, you inject the sample into the medium in the rotor. Again, the details depend on the rotor and the type of operation as detailed in the rotor manual.
Page 61: Stopping The Run
Operations Zonal and Continuous Flow Operation Stopping the Run When you have completed all the defined unloading steps, select the Button to bring the rotor Stop to a halt. Finishing the Run When the rotor comes to a halt, unmount it and perform all the cleanup and follow-up steps given in the rotor manual.
Page 62 Operations Zonal and Continuous Flow Operation 3-14 PN B08115AF...
Page 63: Configuration
• Managing Reports Managing Your Network You can add the Optima XPN to your network, enabling email, file transfer, and printing to a network printer. You can also enable VNC (Virtual Network Connection) or API (Application Programming Interface) to allow the system to receive instructions from a remote application.
Page 64: Selecting A Printer
Use this option to select a USB or network printer for the instrument. The system automatically sends all print requests to the selected printer. NOTE Printer drivers must be installed by a Beckman Coulter Field Service representative. Select the Button on the Header Bar to display the page.
Page 65: Setting Up Email
Configuration Managing Your Network Setting Up Email Use this option to configure email that can be sent from the instrument. The instrument will send diagnostic notifications to the email account of all users with an email address entered in their user profile.
Page 66: Setting Up Vnc
Configuration Managing Your Network Setting Up VNC Use VNC (Virtual Network Connection) to connect to the instrument from a laptop or other remote device. Select the Button on the Header Bar to display the page. Menu Menu Select to display the page.
Page 67: Managing Users
Managing Users Managing Users You can require users to log in prior to using the Optima XPN. You can use the login to build a run log for each user, and to control access to the system. The system includes three user levels that grant different ranges of system access.
Page 68: Requiring Login
Configuration Managing Users You can also use the Manage Users page to edit or delete users, to copy existing user information for a new user, or to edit program permissions for Operator-level users. Requiring Login Select the Button on the Header Bar to display the Page.
Page 69: Pin Expiration And Logout Timer
Configuration Managing Users PIN Expiration and Logout Timer The system defaults to expire PINs every 60 days, and to log out users after two minutes of inactivity. Follow these steps to change the defaults. Select the Button on the Header Bar to display the Page.
Page 70: User Options
Configuration Managing Rotors User Options When Login is required, the User Options page becomes active. From this page, users can change their PIN, add or change their email and phone number, and select an avatar and background to appear on their Home page. Select the button on the Header Bar to display the page.
Page 71: Adding Rotors
Configuration Managing Rotors When you enable , all users must select a rotor from the rotor library before Require Rotor Selection a run. To make rotors available for selection, you must add rotors to the rotor library Adding Rotors To add rotors to the rotor library, select the button on the Header Bar to display the Menu Menu...
Page 72: Managing Reports
Configuration Managing Reports Managing Reports Optima XPN automatically saves run history data associated with each run. The run history generated includes: • User information • Start date and time of the run • End date and time of the run •...
Page 73: Run History Filter
Configuration Managing Reports Figure 4.2 Run History Filter Select the button, then select the button. Filter by Date From Figure 4.3 Set From Date and Time On the page, use the up and down arrow buttons to set the parameters Set From Date and Time for the Month, Day, Year, Hour, Minute, and AM/PM.
Page 74: Graph Data
Configuration Managing Reports On the page, select OK to return to the page. Run History Filter Run History You can now Print, Graph or Export the filtered run history. Figure 4.4 Run History Graph Data To graph data for a run in progress: Select the button on the side menu of the page.
Page 75: Print Data
Configuration Managing Reports Figure 4.6 Run History Button On the page, select the Date of the run from the left column. Run History Select the tab at the bottom of the page. Graph On the page, select Historical Run Data Options On the page, choose one of three options:...
Page 76: Export Data
Configuration Managing Reports Figure 4.8 Run Graph Button On the page, select the tab. Historical Run Data Options On the page, choose one of three options: , or Run Graph Options Fit To Screen Auto Scroll Manual is the default.) Then select Scroll Fit To Screen Select the...
Page 77: Run History Button
When the system reads the USB drive, select it from the Available Drives list, and select the button. Export Optima XPN will then export the currently listed run histories to the USB drive. To manually export run history data to a mapped network: Select the button on the side menu of the page.
Page 78: Auto Print And Auto Export Run History Data
The small box in the button will turn green to indicate it is enabled. Auto Print When a run is completed, Optima XPN will automatically print the run history data to the printer previously configured to the program. To deactivate the function, follow the previous four steps.
Page 79: Run Comments
Configuration Managing Reports Select the button. Options Select the tab. Reports Select the button. Auto Export The small box in the button will turn green to indicate it is enabled. Auto Export When a run is completed, the instrument automatically exports the run history data to the network (if previously configured to the program) as a .csv file (a simple text spreadsheet) and an .xml file (a simple, structured text format).
Page 80: E-Signature
Configuration Managing Reports The system displays the page. Enter a comment and select to start the Before Run Comment run. You can leave the comment field blank, but you must select to start the run. If you select , the run will not start. Cancel Figure 4.13 Before Run Comment When the run stops or you select the...
Page 81: Signature,
Configuration Managing Reports The small box in the button will turn green to indicate it is enabled. E-Signature To disable the function, follow the previous four steps. E-Signature The small box in the button will turn grey to indicate it is disabled. E-Signature To use E-Signature: After a run is complete, select the...
Page 82 Configuration Managing Reports The note and E-Signature will be added to the Summary on the page. See the Run History Run History Page for more information. 4-20 PN B08115AF...
Page 83: Programs
CHAPTER 5 Programs The Optima XPN includes the functionality to create and store programs in the centrifuge memory. A program is a series of steps containing parameters for a run. Programs are retained in the centrifuge memory until they are deleted.
Page 84: Unnamed Program
Programs Creating Programs Figure 5.3 Select Program On the page, appears as the title. To name the new program now, New Program •Unnamed• select on the screen. •Unnamed• Figure 5.4 Unnamed program On the page, use the keyboard to name it, then select to save it.
Page 85: New Program Name
Programs Creating Programs Figure 5.5 New Program Name On the page, select the button to set the Acceleration and Deceleration New Program Accel Decel profiles. On the page, set the profiles, then select to save Acceleration/Deceleration Profiles them and return to the previous page. Figure 5.6 Set Acceleration/Deceleration Profiles To specify the rotor and labware, select the button.
Page 86: New Step
Programs Creating Programs Figure 5.7 Select Rotor and Labware On the page, select the button. New Program New Step Figure 5.8 New Step a. Select the button at the top of the page, then use the keypad to set the speed. The Set Speed speed must be at least 1,000 RPM up to the maximum speed for selected rotor and labware, or if no rotor is specified, the rated speed for the instrument.
Page 87: Running Programs
Programs Running Programs The new step appears in a numbered sequence for the run. From this point, more steps can be added to the run, edited, or deleted using the , and buttons. New Step Edit Step Delete Step Figure 5.9 New Step in a numbered sequence Running Programs Select from the side menu on the...
Page 88: Editing Programs
Programs Editing Programs Editing Programs You must have Administrator or Super User access to modify any part of a program, which includes the Steps, Acceleration/Deceleration rates, and Rotor and Labware. You can edit a program only when it is not running. To edit a program: Select from the side menu on the...
Page 89 Programs Deleting Programs On the page, select the program you want to delete, then select the Select Program Delete button. The system displays a confirmation message. Select to delete the program, or select cancel the deletion. NOTE A deleted program name cannot be reused. PN B08115AF...
Page 90 Programs Deleting Programs PN B08115AF...
Page 91: Chapter 6: Calculations
CHAPTER 6 Calculations Using Calculations Use Calculations to perform a variety of calculations commonly used in ultracentrifugation. These calculations help simplify run preparation. The Calculations function includes the following options: Reduce Rotor Speed For Dense Solutions • Reduce Rotor Speed For Precipitating Solutions •...
Page 92 Calculations Using Calculations Figure 6.1 Menu Page The system displays the Calculations page. PN B08115AF...
Page 93: Reduce Rotor Speed For Dense Solutions
Calculations Using Calculations Figure 6.2 Calculations Page Reduce Rotor Speed For Dense Solutions This function calculates the reduced run speed when centrifuging a solution with a density greater than the allowable density rating of the rotor (as listed in the applicable rotor manual) to protect the rotor from excessive stresses due to the added load.
Page 94: Reduce Rotor Speed For Precipitating Solutions
Calculations Using Calculations Figure 6.3 Reduce Rotor Speed for Dense Solutions Page Select to select a rotor and labware combination, then select Select Rotor and Labware Select to enter the average density of the tube contents in g/mL, then select Average Density is displayed.
Page 95: Sedimentation Coefficient From Run Data
Calculations Using Calculations Figure 6.4 Reduce Rotor Speed for Precipitating Solutions Page Select to select a rotor and labware combination, then select Select Rotor and Labware Select to enter the average density of the tube contents in g/mL, then select Average Density is displayed.
Page 96 Calculations Using Calculations Figure 6.5 Determine Sedimentation Coefficient From Run Data Page Select to select a rotor and labware combination, then select Select Rotor and Labware Select to enter the material location as the percentage down the tube from the Material Location meniscus, then select Select...
Page 97: Sedimentation Coefficient From Molecular Mass
Calculations Using Calculations Select and enter the values for the speed, time, and temperature, then Speed/Time/Temperature select Note the sedimentation coefficient, then select to return to the Calculations page. Done Sedimentation Coefficient From Molecular Mass This function calculates the sedimentation coefficient from the given molecular weight of the macromolecule.
Page 98: Pelleting Time
Calculations Using Calculations Select to enter the molecular mass or molecular length of Molecular Mass Molecular Length the molecule, then select NOTE The Molecular Mass button changes to Molecular Length for the DNA or RNA macromolecules. is displayed. Sedimentation Coefficient Note the calculated sedimentation coefficient, then select to return to the Calculations Done...
Page 99: Refractive Index
Calculations Using Calculations Select to select a rotor and labware combination, then select Select Rotor and Labware Select to enter the sedimentation coefficient of the particles being Sedimentation Coefficient separated, then select Select to enter the speed in RPM or RCF. Speed (RPM/RCF) NOTE This option is not enabled until a rotor is selected.
Page 100: Concentration Measures
Calculations Using Calculations Figure 6.8 Calculate Refractive Index Page Enter the value for one of the following options: • Refractive Index • Density • Molarity The remaining two parameters are calculated and displayed. Note the calculated parameters, then select to return to the Calculations page. Done Concentration Measures This function converts between concentration measures.
Page 101: Calculate Concentration Measures Page
Calculations Using Calculations Figure 6.9 Calculate Concentration Measures Page Select a gradient solute (CsCl or Sucrose). Enter the value for one of the following options: • Density • Molarity (weight-to-volume concentration) • %w/v (weight-to-weight concentration) • %w/w The remaining three parameters are calculated and displayed. Note the calculated parameters, then select to return to the Calculations page.
Page 102 Calculations Using Calculations 6-12 PN B08115AF...
Page 103: Chapter 7: Simulations
CHAPTER 7 Simulations Using Simulations The Simulations page creates simulations of a variety of run conditions commonly used in ultracentrifugation. These simulations help simplify run preparation. (Efficient Sedimentation Program) predicts optimum run conditions for the separation of particles over a wide variety of rotor and tube combinations. The program calculates the movement of particles in solution as a function of speed and the geometry of different rotor and tube combinations.
Page 104 Simulations Using Simulations Figure 7.1 Menu Page The system displays the Simulations page. PN B08115AF...
Page 105: Esp Rna Pelleting Best Run
Simulations Using Simulations Figure 7.2 Simulations Page ESP RNA Pelleting Best Run This function simulates separation of RNA molecules in the range 0.1 to 3.0 kb from the chromosomal DNA through a cushion of 5.7 M CsCl at 25°C in a swinging bucket rotor. In the simulation, the sample suspended in 2.91 M Cesium Chloride (CsCl) containing 4 M Guanidine Thiocyanate (GuSCN) layered over a CsCl cushion occupying one-third of the tube volume.
Page 106: Esp Rna Pelleting In Csci With Guscn, Optimized For Purity
Simulations Using Simulations Figure 7.3 ESP RNA Pelleting in CsCI with GuSCN, Optimized for Purity Page Select to select a rotor and labware combination, then select Rotor Labware NOTE For this simulation, only swinging bucket rotors are available. Select the button with the blue indicator to enter the value for the molecular weight of RNA to be separated, then select Select .
Page 107: Esp Rna Pelleting Fast Run
Simulations Using Simulations Select to transfer the current simulated run settings to the instrument settings for a Transfer live run. Select to save the current simulated run settings as a named run program, which Save can be run at a later time. ESP RNA Pelleting Fast Run This function simulates separation of RNA molecules in the range 0.1 to 3.0 kbp from the sheared chromosomal DNA molecules through a cushion of 5.7 M CsCl at 25°C in a swinging bucket rotor.
Page 108: Esp Pelleting Run
Simulations Using Simulations Select the button with the blue indicator to enter the value for the molecular weight of RNA to be separated, then select Select . The simulation is displayed on the graph. Simulate The following describes the graph: •...
Page 109: Esp Pelleting Separation Page
Simulations Using Simulations Figure 7.5 ESP Pelleting Separation Page Select to select a rotor and labware combination, then select Rotor Labware Select the button with the red indicator to enter the value for Sedimentation Coefficient for the particle to be pelleted, then select NOTE A sedimentation coefficient value is usually reported as s20,w, which means at 20°C in water.
Page 110: Esp Rate Zonal Run
Simulations Using Simulations Select to transfer the current simulated run settings to the instrument settings for a Transfer live run. Select to save the current simulated run settings as a named run program, which Save can be run at a later time. ESP Rate Zonal Run This function simulates particle separation achieved with rate zonal separation, which is a function of the sedimentation coefficient and density of the particles, and the viscosity of the gradient...
Page 111: Esp Plasmid Run
Simulations Using Simulations Select to select the gradient of the Sucrose solution, then select Gradient For more information on gradients, refer to the Calculations chapter. Select to enter the density in g/mL of the particles to be separated, then select Density Select to enter the run temperature, then select...
Page 112: Esp Optimized Plasmid Dna Separation Page
Simulations Using Simulations Figure 7.7 ESP Optimized Plasmid DNA Separation Page Select to select a rotor and labware combination, then select Rotor Labware Select the button with the blue indicator to enter the value for molecular weight of DNA, then select The same value of molecular weight entered for supercoiled DNA appears in the button with the green indicator, representing the linear (nicked) DNA of the same molecular weight.
Page 113: Substitute Rotor Run
This function converts a set of run settings from one type of rotor and labware to another in order to achieve approximately comparable results and allows conversion of runs from non-Beckman Coulter equipment (rotors from other manufacturers) to run on the Optima XPN. To convert a set of run settings:...
Page 114 • Rmin/Rmax Use this option to select non-Beckman Coulter or other unsupported rotors that are either obsolete or discontinued. Enter values that correspond to meniscus and inside-bottom of the labware positions at speed, respectively. These are the distances from the axis of rotation in millimeters.
Page 115: Menu Page
CHAPTER 8 Functional Pages This chapter describes the functional pages used to control the Optima XPN. They include all of the following: Home Page • Set Speed Page • Set Time Page • Delay Start Page — Set Speed ω²t Time Page •...
Page 116: Reduce Rotor Speed For Dense Solutions Page
Functional Pages The System Tab · Set Date and Time Page – System Log Page – Manage Rotors Page – Add to Rotor Library Page – Diagnostic History Page – Set Sound Page – Custom Sounds Page – Archive Data Page –...
Page 117: Home Page
Functional Pages Home Page Simulations Page — ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page · Select Rotor and Labware Page (Catalog/Library) – ESP RNA Pelleting in CsCl with GuSCN, Optimized for Speed Page · ESP Pelleting Separation Page ·...
Page 118 Functional Pages Home Page Display/Button shows the current rotor speed as a large number and also shows Set Speed the current run speed setting in small characters across the bottom. Select the Set Speed if the w²t mode Display/Button to display the Set Speed Page (or to the Set Speed ω²t Time Page...
Page 119: Home Page (Run In Progress)
Functional Pages Home Page (Run in Progress) Home Page (Run in Progress) Figure 8.2 Home Page (Run in Progress) When an actual run is in progress, the page includes the following information: Home • The Display shows the actual speed of the rotor in RPM or RCF. Set Speed •...
Page 120: Set Speed Page
Functional Pages Set Speed Page Set Speed Page Figure 8.3 Set Speed Page (No Rotor Selected) Figure 8.4 Set Speed Page (Rotor and Labware Selected) To set the speed for the next run or change the speed of the run in progress, select the Set Speed Display/Button on the Home Page...
Page 121: Select Rotor And Labware Page
Functional Pages Select Rotor and Labware Page (Library) • The button displays the Select Rotor and Labware Page (Library) page. Select Rotor • The button discards your changes and dismisses the page. Cancel • The button saves your entry and dismisses the page. Select Rotor and Labware Page (Library) Figure 8.5 Select Rotor and Labware Page (Library) To select a rotor for the next run, select the...
Page 122: Set Time Page
Functional Pages Set Time Page Set Time Page Figure 8.6 Set Time Page To set the duration for the next run or change the duration of the run in progress, select the e Display/Button on the Home Page or the Footer Bar to display the page.
Page 123: Delay Start Page
Functional Pages Delay Start Page Delay Start Page Figure 8.7 Delay Start Page To set a future start or end time for the next run, select the button on the Set Time Page Delay Start or the Set Speed ω²t Time Page to display the page.
Page 124: Set Speed Ω²T Time Page
Functional Pages Set Speed ω²t Time Page Set Speed ω²t Time Page Figure 8.8 Set Speed ω²t Time Page When the ω²t mode is active and you select either the button or the button on Set Speed Set Time Home Page or the Footer Bar, the system displays the page.
Page 125: Set Temperature Page
Functional Pages Set Temperature Page Set Temperature Page Figure 8.9 Set Temperature Page To set the temperature for the next run or change the temperature for the run in progress, select button on the Home Pageor the Footer Bar to display the page.
Page 126: Set Acceleration/Deceleration Profiles Page
Functional Pages Set Acceleration/Deceleration Profiles Page Set Acceleration/Deceleration Profiles Page Figure 8.10 Set Acceleration/Deceleration Profiles Page To choose profiles for acceleration and deceleration, select the Display/Button in the Accel/Decel Footer Bar of the Home Page to display the page. Set Acceleration/Deceleration Profiles In addition to the Header Bar and the Footer Bar, the page Set Acceleration/Deceleration Profiles...
Page 127: Example Acceleration And Deceleration Profiles
Functional Pages Set Acceleration/Deceleration Profiles Page Example Acceleration and Deceleration Profiles The values associated with each acceleration and deceleration profile are given in the tables below. NOTE The values shown should be considered as approximations for time and rpm. Acceleration Deceleration Profile # Time...
Page 128: Login Page
Functional Pages Login Page Login Page Figure 8.11 Login Page If the user login requirement has been enabled for the instrument, you must log in to the system use it. Select the button on the Home Page to display the page.
Page 129: Select Program Page
Functional Pages Select Program Page Select Program Page Figure 8.12 Select Program Page To select or manage your run programs, select the button on the Home Page to display the Program page. Operator-level users cannot create or change programs. Select Program In addition to the Header Bar and the Footer Bar, the page contains the following Select Program...
Page 130: New/Edit/View Program Page
Functional Pages New/Edit/View Program Page New/Edit/View Program Page Figure 8.13 New Program Page Figure 8.14 Edit Program Page 8-16 PN B08115AF...
Page 131: View Program Page
Functional Pages New/Edit/View Program Page Figure 8.15 View Program Page To create a run program, select the New button on the Select Program Page to display the page. To edit or view a run program, select the program and select the button Program Edit...
Page 132: Select Rotor And Labware Page
Functional Pages Select Rotor and Labware Page (Catalog) Select Rotor and Labware Page (Catalog) Figure 8.16 Select Rotor and Labware Page (Catalog) To select a type of rotor for a run program, select the button on the New Program Rotor/Labware Page or the Edit Progam Page...
Page 133: New/Edit Step Page
Functional Pages New/Edit Step Page New/Edit Step Page Figure 8.17 New Step Page Figure 8.18 Edit Step Page To create a step within a run program, select the button on the New Program Page or the New Step Edit Progam Page to display the page.
Page 134: Program Log Page
Functional Pages Program Log Page • The field shows the default or current step Set Temperature setting in °Celsius. Select Set Temp the field and use the keypad to change the entry as required. • Use the Keypad to change the settings as required. You can use the keys to make Clear Back...
Page 135: Export Page
Functional Pages Export Page • Select to print the program log. Print • Select to export the program log. The system displays the Export Page. Export Export Page Figure 8.20 Export Page Different kinds of information can be exported from the XPN to a USB device or network drive. All Export pages function in the same manner.
Page 136: Authorize Users Page
Functional Pages Authorize Users Page Authorize Users Page Figure 8.21 Authorize Users Page To manage the list of users with permission to run a program, select the program and select the button on the Select Program Page to display the page.
Page 137: Import Page
Functional Pages Import Page Import Page Figure 8.22 Import Page Many kinds of information can be imported to the XPN from a USB device or network drive. All Import pages function in the same manner. NOTE Do not remove a USB drive while a data transfer is in progress. In addition to the Header Bar and the Footer Bar, the page contains the following elements: Import...
Page 138: Real-Time Run Data/Historical Run Data Page
Functional Pages Real-Time Run Data/Historical Run Data Page Real-Time Run Data/Historical Run Data Page Figure 8.23 Real-Time Run Data Page Figure 8.24 Historical Run Data Page Select the button on the side menu of the Home Page to display the Run Graph Real-Time Run Data page.
Page 139: Run Graph Options Page
Functional Pages Run Graph Options Page Figure 8.25 Run Graph Button Select a run log from the Run History Page and select the button to display the Graph Historical Run page for the selected run. Data In addition to the Header Bar and the Footer Bar, the Real-Time Run Data page contains the following elements: •...
Page 140: Run History Page
Functional Pages Run History Page • The button displays the last 10 minutes of the run, with a scroll bar beneath the Manual Scroll run graph to scroll through the entire run. • Select the button to discard your changes and return to the previous page. Cancel •...
Page 141: Run History
Functional Pages Run History Page Figure 8.28 Run History Page (E-Signature Enabled) Select the button on the side menu of the Home Page to display the page. Run History Run History Figure 8.29 Run History Button In addition to the Header Bar and the Footer Bar, the page contains the following Run History elements:...
Page 142: Run History Filter Page
Functional Pages Run History Filter Page • The button displays the Run History Filter Page. Filter • The button prints all the listed run logs. (Use to narrow the list.) Print Filter • The button displays the Historical Run Data Page for the selected run log.
Page 143: Before Run/After Run Comment Page
Functional Pages Before Run/After Run Comment Page Before Run/After Run Comment Page Figure 8.31 Before Run Comment Page If your system has Run Comments enabled (see The Reports Tab), it displays the Before Run page when you start a run, and the page when a run ends.
Page 144: Menu Page
Functional Pages Menu Page Menu Page Figure 8.32 Menu Page Select the button in the Header Bar to display the page. Use this page to configure or use Menu Menu XPN options. • The button displays the System Options Page. Options •...
Page 145: System Options Page
Functional Pages System Options Page System Options Page Figure 8.33 System Options Page, Basic Tab Select the button in the page to display the page. Use this page to Options Menu System Options configure the system option settings. The System Options page is organized into five tabs. If you have user login enabled, many of the options are restricted to Admin-level users.
Page 146: The System Tab
Functional Pages System Options Page The System Tab Figure 8.34 System Options Page, System Tab • The button displays the page. Enter the system name and Set System Name Set System Name select OK to return to the page. System Options •...
Page 147: The Network Tab
Setup Email Page. Setup Email • The button displays the Setup VNC Page. Setup VNC • The button enables and disables the Applications Programming Interface for remote Enable API devices. Contact your Beckman Coulter representative for more information. PN B08115AF 8-33...
Page 148: The Users Tab
Functional Pages System Options Page The Users Tab Figure 8.36 System Options Page, Users Tab • The button displays the Manage Users Page. Manage Users • The button displays the PIN Expiration page. Enter the number of days that you PIN Expiration want PINs to remain valid.
Page 149: The Reports Tab
Functional Pages System Options Page The Reports Tab Figure 8.37 System Options Page, Reports Tab • The button enables and disables the Signature function for run logs. See E-Signature Signature for more information. • The button enables and disables automatic printing of run logs at the completion of Auto Print each run.
Page 150: Select Language Page
Functional Pages Select Language Page Select Language Page Figure 8.38 Select Language Page Select the button on the Basic tab of the System Options Page to display the Select Language Select page. Use this page to choose the language and date, time and number format the Language instrument uses in operations.
Page 151: User Options Page
Functional Pages User Options Page User Options Page Figure 8.39 User Options Page Select the button on Basic tab of the System Options Page to display the User Options User Options page. Use this page to configure personal options and information for your User ID on the XPN. NOTE You can display this page only if Require Login is enabled (see The Users...
Page 152: Reset User Pin Page
Functional Pages Reset User PIN Page Reset User PIN Page Figure 8.40 Reset User PIN Page Select the field on the User Options Page to display the page. Use this page to Reset User PIN change the PIN for your user ID. In addition to the Header Bar and the Footer Bar, the page contains the following Reset User PIN...
Page 153: Select Image Page
Functional Pages Select Image Page Select Image Page Figure 8.41 Select Image Page (Avatar) Figure 8.42 Select Image Page (Background) Select the button or the button on the User Options Page to display the Set Avatar Set Background page. Use this page to select or import an avatar or background for your user ID on Select Image the XPN.
Page 154: Set Date And Time Page
Functional Pages Set Date and Time Page In addition to the Header Bar and the Footer Bar, the page contains the following Select Image elements: • The Image list shows all the available avatar or background images. • Select to import an image from a network drive or USB device. The system Import Image displays the Import...
Page 155: System Log Page
Functional Pages System Log Page and PM notation. With some language/country selections, the 12 hour option may be unavailable and the buttons will not be visible. Select Time Format • The button discards your changes and returns to the System Options Page.
Page 156: Manage Rotors Page
Functional Pages Manage Rotors Page Manage Rotors Page Figure 8.45 Manage Rotors Page To add or delete rotors from your rotor library, select the button on the System tab Manage Rotors of the System Options Page to display the page. See Managing Rotors for more Manage Rotors...
Page 157: Add To Rotor Library Page
Functional Pages Add to Rotor Library Page Add to Rotor Library Page Figure 8.46 Add to Rotor Library Page To add a rotor to your rotor library, select the button on the Manage Rotors Page to display the page. Add to Rotor Library In addition to the Header Bar and the Footer Bar, the page contains the Add to Rotor Library...
Page 158: Diagnostic History Page
Functional Pages Diagnostic History Page Diagnostic History Page Figure 8.47 Diagnostic History Page Select the button on the System tab of the System Options Page to display the Diagnostic History page. Use this page to review and export the details of incidents that caused a Diagnostic History diagnostic message on the instrument (warnings and error messages).
Page 159: Set Sound Page
Functional Pages Set Sound Page Set Sound Page Figure 8.48 Set Sound Page Select the button on the System tab of the System Options Page to display the Set Sound Set Sound page. Use this page to set the volume and enable or disable the key click option. In addition to the Header Bar and the Footer Bar, the page contains the following Set Sound...
Page 160: Custom Sounds Page
Functional Pages Custom Sounds Page Custom Sounds Page Figure 8.49 Custom Sounds Page Select the button on the System tab of the System Options Page to display the Custom Sounds page. Use this page to import custom sounds for various system events. See Audible Custom Sounds Sounds...
Page 161: Archive Data Page
Functional Pages Archive Data Page Archive Data Page Figure 8.50 Archive Data Page Select the button on the System tab of the System Options Page to display the Archive Data Archive page. Use this page to configure export of different types of system information. Data NOTE Run History and Diagnostic History cannot be imported back into the system.
Page 162: Setup Network Page
Functional Pages Setup Network Page Setup Network Page Figure 8.51 Setup Network Page Select the button on Network tab of the System Options Page to display the Setup Network Setup page. Use this page to configure the network connection. Network In addition to the Header Bar and the Footer Bar, the page contains the following Setup Network...
Page 163: Select Printer Page
Select Printer elements: • The Printer list shows the configured printers on the network or physically connected to the instrument. Printers must be configured by Beckman Coulter Field Service. Select the printer to use. • You can select to send a test page to the selected printer.
Page 164: Setup Email Page
Functional Pages Setup Email Page Setup Email Page Figure 8.53 Setup Email Page Select the button on the Network tab of the System Options Page to display the Setup Email Setup page. Use this page to configure settings for email sent from the instrument. When email is Email configured, the instrument sends diagnostic notifications to all users with email addresses defined in their user profiles.
Page 165: Setup Vnc Page
Functional Pages Setup VNC Page • The button accepts your changes and returns to the System Options Page. Save Setup VNC Page Figure 8.54 Setup VNC Page Select the Setup VNC button on the Network tab of the System Options Page to display the Setup page.
Page 166: Manage Users Page
Functional Pages Manage Users Page Manage Users Page Figure 8.55 Manage Users Page To add, delete or edit your users, select the button on the Users tab of the System Options Program Page to display the page. Manage Users In addition to the Header Bar and the Footer Bar, the page contains the following Manage Users elements:...
Page 167: Add/Edit User Page
Functional Pages Add/Edit User Page Add/Edit User Page Figure 8.56 Add User Page To add a user profile to the system, select the button on the Manage Users Page to display the page. Add User To edit an existing user profile, select the user ID and select on the Manage Users Page Edit...
Page 168: Authorize Programs Page
Functional Pages Authorize Programs Page • For Operator-level user profiles, you can select the button to add Authorize Programs permission to run programs to the user profile. The system displays the Authorize Programs Page. • The button discards your changes and returns to the Manage Users Page.
Page 169: References Page
Functional Pages References Page References Page Figure 8.58 References Page Select the button in the Menu Page to display the page. Use this page to view References References reference materials. In addition to the Header Bar and the Footer Bar, the page contains the following References elements:...
Page 170: Rotor Catalog Page
Functional Pages Rotor Catalog Page Rotor Catalog Page Figure 8.59 Rotor Catalog Page Select the button on the References Page to display the page. Use this Rotor Catalog Rotor Catalog page to examine the detailed specifications for all the rotors compatible with the XPN. In addition to the Header Bar and the Footer Bar, the page contains the following Rotor Catalog...
Page 171: Compatible Tubes For Rotor Page
Functional Pages Compatible Tubes for Rotor Page Compatible Tubes for Rotor Page Figure 8.60 Compatible Tubes for Rotor Page Select the button on the Rotor Catalog Page to display the page. Labware Compatible Tubes for Rotor Use this page to examine the detailed specifications for the labware for the selected rotor. In addition to the Header Bar and the Footer Bar, the page contains the Compatible Tubes for Rotor...
Page 172: Labware Catalog Page
Functional Pages Labware Catalog Page Labware Catalog Page Figure 8.61 Labware Catalog Page Select the button on the References Page to displays the page. Use Labware Catalog Labware Catalog this page to examine the detailed specifications for labware available for the rotors your instrument can use.
Page 173: Chemical Resistances Page
CAUTION The information provided in the table is from current literature or research done by Beckman Coulter, and is only a guide for the proper selection of materials. No guarantee of safety, based on these recommendations, is expressed or implied.
Page 174 Functional Pages Calculations Page Calculations Page Figure 8.63 Calculations Page Select the button in the Menu Page to display the page. Use this page to Calculations Calculations perform a variety of calculations commonly used in ultracentrifugation. These calculations help simplify run preparation. In addition to the Header Bar and the Footer Bar, the page contains the following Calculations...
Page 175: Reduce Rotor Speed For Dense Solutions Page
Functional Pages Reduce Rotor Speed for Dense Solutions Page Reduce Rotor Speed for Dense Solutions Page Figure 8.64 Reduce Rotor Speed for Dense Solutions Page To display the page, select the button Reduce Rotor Speed for Dense Solutions For Dense Solutions on the Calculations Page.
Page 176: Reduce Rotor Speed For Precipitating Solutions Page
Functional Pages Reduce Rotor Speed for Precipitating Solutions Page Reduce Rotor Speed for Precipitating Solutions Page Figure 8.65 Reduce Rotor Speed for Precipitating Solutions Page To display the page, select the Reduce Rotor Speed for Precipitating Solutions For Precipitating button on the Calculations Page.
Page 177: Determine Sedimentation Coefficient From Run Data Page
Functional Pages Determine Sedimentation Coefficient from Run Data Page Determine Sedimentation Coefficient from Run Data Page Figure 8.66 Determine Sedimentation Coefficient from Run Data Page To calculate the sedimentation coefficient from provided run data, select the button From Run Data on the Calculations Page to display the...
Page 178: Determine Sedimentation Coefficient From Molecular Mass Page
Functional Pages Determine Sedimentation Coefficient from Molecular Mass Page Determine Sedimentation Coefficient from Molecular Mass Page Figure 8.67 Determine Sedimentation Coefficient from Molecular Mass Page To calculate the sedimentation coefficient from molecular mass, select the From Molecular Mass button on the Calculations Page to display the Determine Sedimentation Coefficient from Molecular...
Page 179: Calculate Pelleting Time Page
Functional Pages Calculate Pelleting Time Page Calculate Pelleting Time Page Figure 8.68 Calculate Pelleting Time Page To calculate particle pelleting time, select the button on the Calculations Page Pelleting Time display the page. See Pelleting Time for more information. Calculate Pelleting Time In addition to the Header Bar and the Footer Bar, the Calculate Pelleting Time page contains the following elements: •...
Page 180: Calculate Concentration Measures Page
Functional Pages Calculate Concentration Measures Page Calculate Concentration Measures Page Figure 8.69 Calculate Concentration Measures Page To convert concentration measures between density, molarity, %weight/volume, or %weight/weight, select the button on the Calculations Page to display the Concentration Measures page. See Concentration Measures for more information.
Page 181: Calculate Refractive Index Page
Functional Pages Calculate Refractive Index Page Calculate Refractive Index Page Figure 8.70 Calculate Refractive Index Page To calculate the refractive index, density, and molarity for CsCl at 20°C, select the Refractive Index button on the Calculations Page to display the page.
Page 182: About Page
Functional Pages About Page About Page Figure 8.71 About Page page appears when you select the button on the Home Page. This page presents About About system information about your instrument. In addition to the Header Bar and the Footer Bar, the page contains the following operating About elements:...
Page 183: Zonal/Continuous Flow Authorization Page
Functional Pages Zonal/Continuous Flow Authorization Page Zonal/Continuous Flow Authorization Page Figure 8.72 Zonal Authorization Page Select the button on the side menu of the Home Page, or the button on Zonal Mode Zonal Operation Menu Page to display the page. Enter the authorization code (1793) and Zonal Authorization select to use Zonal mode for the next run.
Page 184: Zonal/Continuous Flow Operation Page
Functional Pages Zonal/Continuous Flow Operation Page Zonal/Continuous Flow Operation Page Figure 8.74 Zonal Operation Page Figure 8.75 Continuous Flow Operation Page Enter the correct authorization code from the Zonal Authorization Page to display the Zonal page and enter Zonal mode. Operation Enter the correct authorization code from the Continuous Flow Authorization Page...
Page 185 Functional Pages Zonal/Continuous Flow Operation Page When you use Zonal or Continuous Flow mode, the page or the Zonal Operation Continuous Flow page replaces the Home Page until Zonal or Continuous Flow mode ends. Operation In addition to the Header Bar and the Footer Bar, the page contains Zonal/Continuous Flow Operation the following elements:...
Page 186: Simulations Page
Functional Pages Simulations Page Simulations Page Figure 8.76 Simulations Page Select the button on the Menu Page to display the page. Use this page to Simulations Simulations optimize cent rif i gat ion runs through computer simulation. See Using Simulations for more information.
Page 187: Esp Rna Pelleting In Cscl With Guscn, Optimized For Purity Page
Functional Pages ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page Figure 8.77 ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Page To display the page, select the ESP RNA Pelleting in CsCl with GuSCN, Optimized for Purity Best Run...
Page 188: Select Rotor And Labware Page
Functional Pages Select Rotor and Labware Page (Catalog/Library) concentration of the particles and the density of the CsCl gradient during centrifugation. The x-axis indicates the position of the particles along the length of the tube with the bottom of the tube at the right.
Page 189: (Catalog/Library)
Functional Pages ESP RNA Pelleting in CsCl with GuSCN, Optimized for Speed Page showing the available rotors for the instrument. To return to the Rotor Catalog list, disable the button. Select From Rotor Library • When you select a rotor, the Labware list displays compatible labware. Select a type of labware from the list.
Page 190: Esp Pelleting Separation Page
Functional Pages ESP Pelleting Separation Page • When you have entered the fields, select the button to display the simulated run. The Simulate simulation is graphically displayed. — The blue curve represents the relative concentration of the RNA. — The green curve represents the relative concentration of the DNA contaminant. —...
Page 191: Select Rotor And Labware
Functional Pages ESP Pelleting Separation Page To display the page, select the button on the Simulations ESP Pelleting Separation ESP Pelleting Run Page. Use this page to simulate a separation of a uniform mixture of sample solution into two fractions, a pellet containing the sedimented material and a supernatant solution of the unsedimented material.
Page 192: Esp Optimized Plasmid Dna Separation Page
Functional Pages ESP Optimized Plasmid DNA Separation Page ESP Optimized Plasmid DNA Separation Page Figure 8.81 ESP Optimized Plasmid DNA Separation Page To display the pages, select the button on ESP Optimized Plasmid DNA Separation ESP Plasmid Run Simulations Page. Use this page to simulate an optimized plasmid DNA separation in homogeneous 1.55 g/mL CsCl-EtBr at 25°C.
Page 193: Esp Rate Zonal Separation Page
Functional Pages ESP Rate Zonal Separation Page • The button saves the simulated run settings as a named run program, which can be run at Save a later time. NOTE Saving or transferring the simulated run settings does not save the simulated run inputs, only the current simulated run settings (including the adjustments for a shorter elapsed time, if applicable).
Page 194: Substitute Rotor Run Page
Functional Pages Substitute Rotor Run Page • Enter the sedimentation coefficients for up to three particles of interest by selecting the Sedimentation Coefficient buttons, with the --s designations, at the bottom of the page. • When you have entered the fields, select the button to display the simulated run in Simulate the graph above.
Page 195 Functional Pages Substitute Rotor Run Page In addition to the Header Bar and the Footer Bar, the Substitute Rotor Run page contains the following elements: • The Source Rotor section contains the settings buttons for the original run settings. — Select the button to display the Select Rotor and Labware Page (Catalog/ Rotor/Labware...
Page 196 Functional Pages Substitute Rotor Run Page 8-82 PN B08115AF...
Page 197: Chapter 9: Maintenance And Troubleshooting
Reference documents for detailed instructions on their care. Cleaning The methods and materials used in the following procedures have been tested by Beckman Coulter and will not damage the instrument if used as instructed. CAUTION Before using any other materials or methods, check with Beckman Coulter to verify that they will not damage the instrument.
Page 198: Rotor Chamber
If the instrument and/or accessories are contaminated with radioactive or pathogenic solutions, follow appropriate decontamination procedures as determined by your laboratory safety officer. Refer to Chemical Resistances (publication IN-175), or contact Beckman Coulter Field Service to ensure that the decontamination method does not damage any part of the instrument (or accessories).
Page 199: Diagnostics/User Messages
Maintenance and Troubleshooting Diagnostics/User Messages WARNING Ethanol is a volatile liquid that cannot be used on or near an operating instrument due to fire hazard. Diagnostics/User Messages When a condition arises that requires operator attention, the header bar turns yellow or red. A page displays the diagnostic message.
Page 200: Getting Access To The Sample
Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure If the rotor has stopped spinning when power is restored, the instrument cancels the run and sends a diagnostic message to alert you to that the run was cancelled due to a power failure. Getting Access to the Sample If a power failure lasts for several hours, you may have to remove the sample from the rotor while there is no power to the instrument.
Page 201: Panel Screws And Tabs
Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Figure 9.1 Panel screws and Tabs 1. Panel Tabs 3. Front Panel 2. Top Panel 4. Latch Screws When all three latch screws are loose, use your hand to lift the top panel until it pops loose. Lift the front panel until it is free and tilt the top edge towards you.
Page 202: Internal Parts - Configuration "A" Vents Through The Port Cap
Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure WARNING When working on the inside of the instrument, be careful not to touch any wires or circuitry. Determine the instrument’s vacuum configuration. There are two possible configurations: will vent the vacuum through a vent port cap (refer to Figure 9.2).
Page 203: Internal Parts - Configuration "B" Vents Through The Vacuum Solenoid Release Screw
Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Figure 9.3 Internal Parts — Configuration “B” vents through the vacuum solenoid release screw 1. Fan Housing 2. Vacuum solenoid release screw 3. Door Interlock Assembly Also refer to To Vent the Chamber- Configuration “A”...
Page 204 Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure Vent the Chamber Although you checked for noise and vibration, there is still a possibility that the rotor is still spinning. If it is still spinning, you can hear a whining noise when you begin to vent the chamber. If you hear the whining noise when you begin to vent the chamber, you must close the port immediately and wait for the rotor to come to a stop.
Page 205: Restoring The Instrument To Operating Condition
Maintenance and Troubleshooting Retrieving Your Sample in Case of Power Failure WARNING NEVER attempt to slow or stop the rotor by hand. With the door open and the rotor stopped, you can retrieve your sample. WARNING Do not attempt to run the instrument before restoring it to a safe operating condition as described in the following section.
Page 206: Storage And Transportation
Maintenance and Troubleshooting Storage and Transportation Storage and Transportation To ensure that the instrument does not get damaged, contact Beckman Coulter Field Service for specific instructions and/or assistance in preparing the equipment for transport or long-term storage. 9-10 PN B08115AF...
Page 207: Supply List
Supply List Supply List Contact Beckman Coulter Sales for assistance ordering parts and supplies. Customers in the United States call 1-800-742-2345. For international contacts, see the website at www.beckmancoulter.com or use the contact numbers on the inside front cover of this book. A partial list of supplies is given below for your convenience.
Page 208 Maintenance and Troubleshooting Supply List 9-12 PN B08115AF...
Page 209: Appendix A: Preinstallation Requirements
Preinstallation Requirements Overview NOTE Do not attempt to install or turn on the power to the Optima XPN. Its purchase price includes installation by Beckman Coulter personnel. Installation by anyone other than authorized Beckman Coulter personnel invalidates the instrument warranty.
Page 210: Ventilation
Preinstallation Requirements Electrical Requirements event that a rotor failure occurs at high rotational speed. If you decide to install the instrument in an area where furniture, equipment, or a wall is within this safety space, you run the risk of damage to anything within this space in the event of a rotor failure.
Page 211: Electrical Connection
A.2) to connect the equipment to earth ground. In regions where the instrument is supplied with an unterminated cord, a plug that meets local electrical and safety requirements must be supplied. (Contact your local Beckman Coulter office for specific information regarding these requirements.) See Table A.1...
Page 212 Preinstallation Requirements Electrical Requirements PN B08115AF...
Page 213: Appendix B: Special Warranty For The Optima Xpn
Any product claimed to be defective must, if requested by Beckman Coulter be returned to the factory, transportation charges prepaid, and will be returned to Buyer with the transportation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all transportation charges.
Page 214 〈 〉 labor and travel. NOTE For details of drive coverage with a service contract, contact your local Beckman Coulter service representative Conditions 1. The drive has been operated only within its rated speed and temperature ranges. 2. The drive unit has not been subjected to unequal loading, improper rotor installation, corrosion from material spilled onto the hub or accumulated in the chamber of the instrument.
Page 215: Appendix C: Diagnostics
CHAPTER 9, Maintenance and Troubleshooting. For any maintenance not covered in this manual, contact Beckman Coulter Field Service for assistance. USA customers can call 1-800-742-2345. For international contacts, see the website at www.beckmancoulter.com or use the contact numbers on the inside front cover of this book.
Page 216 D113 - Touch Screen Touch Screen USB cable not Be sure USB touch screen cable is connected. Not Detected connected If problem persists, call Beckman Coulter Field Service. D114 - Code error Bad checksum on code flash Call Beckman Coulter Field Service.
Page 217 Definition/Result Recommended Action Hard drive is 90% full Backup files soon. A124 - Alert: Hard drive space low Call Beckman Coulter Field Service. D125 - SBC Communications parameters Call Beckman Coulter Field Service. Communications are not compatible A200 - Alert: AC...
Page 218 Table C.1 Diagnostics/User Messages Chart (Continued) Message Definition/Result Recommended Action System +24V supply too high Call Beckman Coulter Field Service. D213 - Power Supply (>26.4VDC) or System +24V supply too low (<21.6VDC) D214 - Power Supply System +5V supply too high Call Beckman Coulter Field Service.
Page 219 3. If the problem persists, call Beckman Coulter Field Service. D407 - Vacuum Vent Vacuum vent solenoid not Call Beckman Coulter Field Service. connected D408 - Vacuum Vent Vacuum vent can not be Call Beckman Coulter Field Service.
Page 220 Diagnostics/User Messages Chart Table C.1 Diagnostics/User Messages Chart (Continued) Message Definition/Result Recommended Action Can temperature out of limits Call Beckman Coulter Field Service. D505 - Temperature Control (<-30°C or >70°C) D506 - Temperature TEM voltage too high Call Beckman Coulter Field Service.
Page 221 3. Verify the rotor load is within limits specified in the rotor manual. 4. If the problem persists, call Beckman Coulter Field Service. D607 - Drive Drive frequency error Call Beckman Coulter Field Service. Performance D608 - Drive Tachometer frequency >103K Call Beckman Coulter Field Service. Performance...
Page 222 Diagnostics Diagnostics/User Messages Chart PN B08115AF...
Page 223: Appendix D: Third Party Contributions And Legal Notices
Beckman Works (including but not limited to copyright in and to Beckman Works, any derivative work based on part or all of Beckman Works, and images, photographs, text, and other information that may be incorporated in Beckman Works), are owned and reserved by Beckman Coulter, Inc. A. AdornedControl, available at <http://www.codeproject.com/KB/WPF/adornedcontrol.aspx>, by Ashley Davis, the standard version included in its binary form linked with other modules.
Page 224 D. VNC Free Edition 4.1.3, Copyright 2002-2008 RealVNC Limited, is included in its unmodified binary form as aggregate work. A copy of this work in source form may be obtained at no cost by contacting Beckman Coulter Technical Support. This work is made available under the following license:...
Page 225 Third Party Contributions and Legal Notices charge for them if you wish), that you receive source code or can get it if you want it, that you can change the software or use pieces of it in new free programs, and that you know you can do these things.
Page 226 Third Party Contributions and Legal Notices To “propagate” a work means to do anything with it that, without permission, would make you directly or secondarily liable for infringement under applicable copyright law, except executing it on a computer or modifying a private copy. Propagation includes copying, distribution (with or without modification), making available to the public, and in some countries other activities as well.
Page 227 Third Party Contributions and Legal Notices You may make, run, and propagate covered works that you do not convey, without conditions so long as your license otherwise remains in force. You may convey covered works to others for the sole purpose of having them make modifications exclusively for you, or provide you with facilities for running those works, provided that you comply with the terms of this License in conveying all material for which you do not control copyright.
Page 228 Third Party Contributions and Legal Notices A compilation of a covered work with other separate and independent works, which are not by their nature extensions of the covered work, and which are not combined with it such as to form a larger program, in or on a volume of a storage or distribution medium, is called an “aggregate”...
Page 229 Third Party Contributions and Legal Notices whether the product has substantial commercial, industrial or non-consumer uses, unless such uses represent the only significant mode of use of the product. “Installation Information” for a User Product means any methods, procedures, authorization keys, or other information required to install and execute modified versions of a covered work in that User Product from a modified version of its Corresponding Source.
Page 230 Third Party Contributions and Legal Notices • d) Limiting the use for publicity purposes of names of licensors or authors of the material; or • e) Declining to grant rights under trademark law for use of some trade names, trademarks, or service marks;...
Page 231 Third Party Contributions and Legal Notices 10. Automatic Licensing of Downstream Recipients. Each time you convey a covered work, the recipient automatically receives a license from the original licensors, to run, modify and propagate that work, subject to this License. You are not responsible for enforcing compliance by third parties with this License.
Page 232 Third Party Contributions and Legal Notices specifically granted under this License. You may not convey a covered work if you are a party to an arrangement with a third party that is in the business of distributing software, under which you make payment to the third party based on the extent of your activity of conveying the work, and under which the third party grants, to any of the parties who would receive the covered work from you, a discriminatory patent license (a) in connection with copies of the covered work conveyed by...
Page 233 Third Party Contributions and Legal Notices AND FITNESS FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION. 16.
Page 234 Third Party Contributions and Legal Notices D-12 PN B08115AF...

Beckman Coulter Optima XPN User Manual

CHAPTER 1 Description

CHAPTER 2: The Touch Screen Interface

CHAPTER 3: Operations

CHAPTER 4 Configuration

CHAPTER 5 Programs

CHAPTER 6: Calculations

CHAPTER 7: Simulations

CHAPTER 8 Pages

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