Beckman Coulter Optima XL Series Instruction Manual
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Beckman Coulter Optima XL Series Instruction Manual

Beckman Coulter Optima XL Series Instruction Manual

Preparative ultracentrifuge
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TM
Optima
™
Preparative Ultracentrifuge
Instruction Manual
PUBLISHED BY THE SPINCO BUSINESS CENTER OF BECKMAN COULTER, INC., PALO ALTO, CALIFORNIA 94304
XL Series
LXL-IM-10

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  • Page 1 LXL-IM-10 Optima XL Series ™ Preparative Ultracentrifuge Instruction Manual PUBLISHED BY THE SPINCO BUSINESS CENTER OF BECKMAN COULTER, INC., PALO ALTO, CALIFORNIA 94304...
  • Page 2 Symbol Symbol Symbole Title Titel Titre Titulo Símbolo Simbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung) Marche (mise sous tension)
  • Page 3 Safety During Installation and/or Maintenance This instrument is designed to be installed by a Beckman Coulter Field Service represen- tative. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field Service representative must reinstall and relevel the instrument in its new location.
  • Page 4 Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this instrument. • Do not exceed the maximum rated speed of the rotor in use. • NEVER attempt to slow or stop a rotor by hand.
  • Page 5 LXL-IM-10 May 2001 Optima XL Series ™ Preparative Ultracentrifuge Instruction Manual © 2001 Beckman Coulter, Inc. Printed in the U.S.A. PUBLISHED BY THE SPINCO BUSINESS CENTER OF BECKMAN COULTER, INC., PALO ALTO, CALIFORNIA 94304...
  • Page 6 LISTED I.S. EN ISO 9001...

  • Page 7: Table Of Contents

    Contents Page INTRODUCTION Instrument Certification ........Introduction .
  • Page 8 Contents Page SECTION 5: THE PRINTER Printer Menu Window ........5-1 Menu Options .
  • Page 9 Illustrations Page Figure 1-1. Optima XL Control Head ........1-1 Figure 1-2.
  • Page 10 Contents Page Figure 6-1. The CALCULATIONS MENU Screen ......6-1 Figure 6-2. Conversions Between Speed and Relative Centrifugal Force ..6-3 Figure 6-3.

  • Page 11: Introduction

    Introduction INSTRUMENT CERTIFICATION To assure full system quality, Beckman Coulter Optima™ Series XL preparative ultracentrifuges have been manufactured in an NSAI-registered ISO 9001 facility. They have been designed and tested to meet the laboratory equipment standards and regulations (only when used with Beckman Coulter rotors) of: •...

  • Page 12: Scope Of This Manual

    To obtain additional copies of this manual, related rotor manuals, and other referenced publications, contact: Beckman Coulter, Inc. Technical Publications Department 1050 Page Mill Road Palo Alto, CA 94304, U.S.A.

  • Page 13: Conventions

    Introduction CONVENTIONS Certain symbols are used in the manual to call out safety related and other important information. These are reproduced and described below. NOTE Used to call attention to important information that should be followed during installation, use, and/or servicing of this equipment.

  • Page 14: Specifications

    Introduction CANADIAN REGULATIONS This digital apparatus does not exceed the Class A limits for radio noise emis- sions from digital apparatus as set out in the radio interference regulations of the Canadian Department of Communications. Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites applicables aux appareils numériques de Classe A pre- scrites dans le reglement sur le brouillage radioelectrique édicté...
  • Page 15 Vacuum ....diffusion pump in series with a mechanical pump reduces chamber pressure to below 5 microns (0.7 Pa) Instrument Classification ..S (uses all Beckman Coulter preparative rotors except Types 35 and 42.1 with serial numbers 1299 or lower) RS 232C Accessory Port .

  • Page 16: Installation Requirements

    (Copies are also attached to the outside of the shipping container.) The following information is provided in case the Optima XL must be relocated. Contact your Beckman Coulter Field Service representative to adjust and level the instrument if it must be moved. (The pads on each leveling leg are designed to prevent possible rotation of the instrument in the case of a rotor mishap.)
  • Page 17 Introduction 30-ampere Circuit Protector Measured Line Voltage 30-ampere Circuit Protector Earth Ground Wall Outlet: North American Plug Hubbell 9330, Bryant 96-30-FR, or Equivalent (NEMA 6-30 R) To ensure safety, the instrument should be wired to a remote emergency switch (preferably outside the room where the centrifuge is housed, or adjacent to the exit from that room), in order to disconnect the instrument from the main power source in case of a malfunction.
  • Page 18 Introduction Space Locate the Optima XL in a clean, safe, uncluttered environment. Be sure to provide a 5.1-cm (2-in.) clearance on each side of the Optima XL as the feet extend about 5.1 cm (2 in.) beyond the instrument. (The pads under each foot are designed to prevent possible rotation of the instrument in the event of a rotor mishap.) A 15.2-cm (6-in.) clearance is required at the rear of the instru- ment for servicing and to ensure sufficient air ventilation during operation.

  • Page 19: Description

    Description This section describes major instrument components. POWER A circuit breaker, labeled I (on) and O (off), is located on the right side panel of the Optima XL and controls the electrical power to the instrument. Power may be left on (see Standby Mode below) except in the case of an emergency or when maintenance is required.

  • Page 20: Key Switch Positions

    Description STANDBY MODE When the instrument is at rest, it goes into a “Standby Mode”—a condition that requires only minimal power to keep the instrument in a ready state. In standby mode a screen-saver allows the screen to go blank until the instru- ment is returned to its full operational condition, or “Operating Mode,”...

  • Page 21: Control Panel

    Description CONTROL PANEL Figure 1-3 shows the Optima XL control panel, which includes a video display and keys for entering run information or accessing additional instru- ment features. (See Section 2 for a detailed discussion on how to enter run information.) Parameter Activation...

  • Page 22: Printer

    Description DISPLAY The Optima XL is a microprocessor-controlled ultracentrifuge with interac- tive, menu-driven operation. A display shows the current values and run settings as well as a variety of run-related functions, which are accessed by pressing the appropriate keys. A small setscrew on the back of the control head controls the brightness of the video display (see “Adjusting the Brightness of the Video Display”...

  • Page 23: Vacuum System

    Description The rotor chamber is aluminum, coated with a chemical-resistant epoxy finish. The rotor drive spindle, radiometer, photoelectric devices, and safety plate are visible in the bottom of the chamber (see Figure 1-5). Photoelectric Drive Devices Spindle Radiometer Safety Plate Figure 1-5.

  • Page 24: Temperature Sensing

    Description help keep the chamber dry and clean, it is important to keep the door closed whenever possible. In zonal operation, the door may be kept open while the rotor is spinning up to 3000 rpm. The vacuum system is activated when the door is closed (after loading the rotor) and can be vented before the rotor is unloaded, while the rotor is still spinning at up to 3000 rpm.

  • Page 25: Overspeed System

    Description OVERSPEED SYSTEM The overspeed system is a safety feature designed to ensure that the rotor does not exceed its maximum allowable speed. This system includes a photoelec- tric device in the rotor chamber next to the drive spindle (see Figure 1-5) and an overspeed disk on the bottom of the rotor.

  • Page 26: Run Preparation

    Run Preparation This section contains an explanation of the general format of the software and how to enter run information. SCREEN FORMAT The software is presented as a series of windows, either half or full-screen width, containing certain kinds of information at the same place in the display.

  • Page 27: Main Screen

    Run Preparation MAIN SCREEN The Main Screen will usually be displayed—unless you have selected a func- tion that temporarily requires using part or all of the screen (such as viewing the ROTOR CATALOG and SPECIFICATIONS windows). The Main Screen shows the actual conditions of the instrument (CURRENT VALUES) on the left and user-entered values (SETTINGS) on the right (Figure 2-1).

  • Page 28: Figure 2-2. Current Values Window

    Run Preparation CURRENT VALUES SPEED (RPM) TIME REMAINING (HR:MIN) TEMP ω 2 t (DEG C) Vacuum Status Heating or Cooling COOLING VACUUM: OFF Status Line Zonal or Locked Prompt Lines Softkeys ACCEL DECEL PROFILES PROFILES MODE Figure 2-2. CURRENT VALUES Window RUN STATUS MESSAGES The following messages indicate the status of the instrument: COOLING: The instrument is cooling the chamber and/or rotor to the set...

  • Page 29: Figure 2-3. Stop Values Window

    Run Preparation STOP VALUES SPEED (RPM) TIME REMAINING (HR:MIN) TEMP (DEG C) W2T 3.8 7E08 ACCEL DECEL PROFILES PROFILES MODE Figure 2-3. STOP VALUES Window Checking the STOP VALUES can be helpful after a power outage or instru- ment shutdown to learn what the conditions were when the run was terminated.

  • Page 30: Other Screens

    Run Preparation SETTINGS SPEED (RPM) TIME REMAINING (HR:MIN) TEMP (DEG C) ACCEL: ROTOR NO ROTOR DECEL: LOGGING: SELECTED HOLD PROGRAM MORE MODE LIBRARY OPTIONS Figure 2-4. SETTINGS Window, Showing the Default Settings OTHER SCREENS The CURRENT VALUES window and/or the SETTINGS window will sometimes be temporarily replaced by other screens to complete a particular function (such as selecting acceleration or deceleration profiles), to access additional information (such as viewing the ROTOR CATALOG or ROTOR...

  • Page 31: Figure 2-5. Inserting The Key Into The Interlock Switch

    Run Preparation Turn the key to the right (clockwise) to the normal posi- tion for routine closed-door centrifugation. The key should be removed while in this position. Keep the key inserted vertically to run the instrument in locked operation. The key should be removed while in this position.

  • Page 32: Entering Information

    Run Preparation ENTERING INFORMATION Entering or modifying information is referred to as being in the editing mode. • Press the appropriate hardkey or softkey to enter or modify information or to access a specific function. • When you press a parameter key, the appropriate field on the screen will begin to blink, indicating that you can enter or modify the value in that field.

  • Page 33: Parameter Keys

    Run Preparation ✮✷✽✮✻❛ ✼✽✸✹❛ • Press to display the STOP VALUES— (the values that existed when the rotor began deceleration). PARAMETER KEYS ✻✸✽✸✻❛ hardkey is discussed briefly in this section as one of the parameter keys. See Section 4 for a full description of rotor functions. ...
  • Page 34 Run Preparation At speeds between 15 000 and 20 000 rpm, rotor inertia is measured and the rotor energy is calculated for the set speed. If the calculated rotor energy is excessive, the instrument recalculates a maximum permitted set speed and uses this value to avoid possible rotor damage.
  • Page 35 Run Preparation The time begins counting down when the rotor begins to accelerate. (In a zonal run, the time does not begin counting down until the chamber door is closed after loading the rotor while spinning.) When the time remaining reaches zero, the rotor automatically decelerates to a stop.
  • Page 36 Run Preparation The set speed is checked against the rotor’s overspeed disk at 1000 rpm. If the speed setting is greater than that allowed by the overspeed disk, the run will continue uninterrupted, but the rotor speed will be lowered to the maximum speed permitted by the disk.

  • Page 37: Figure 2-6. Acceleration Profiles Window

    Run Preparation You may change the temperature setting at any time except when the instru- ment is using ESP program settings or is in a locked operation. The rotor temperature will be adjusted accordingly. Actual rotor temperature, after equilibration, is controlled to ±0.5°C of the set value.
  • Page 38 Run Preparation 2. Use the keypad or softkeys to select an acceleration profile. ✮✷✽✮✻❛ 3. Press another parameter key or . The ACCEL field in the SETTINGS window stops blinking and the selected profile is displayed.     ✭ ✭ ✭ ✭ ✮ ✮ ✮ ✮ ✬ ✬ ✬ ✬ ✮ ✮ ✮ ✮ ✵ ✵ ✵ ✵ ❈ ❈ ❈ ❈ ✹ ✹ ✹ ✹ ✻ ✻ ✻ ✻ ✸ ✸ ✸ ✸ ✯ ✯ ✯ ✯ ✲ ✲ ✲ ✲ ✵ ✵ ✵ ✵ ✮ ✮ ✮ ✮ ✼ ✼ ✼ ✼ ❛ ❛ ❛ ❛ ¤ ¤ ¤ ¤ SOFTKEY Maximum deceleration, nine slow deceleration profiles, and a “no brake”...
  • Page 39 Run Preparation 2. Do one of the following: ✶✪❁✲✶✾✶❈✭✮✬✮✵❛ Press the softkey for maximum deceleration. Then press ✮✷✽✮✻❛¤ or another parameter key to continue. Use the keypad or arrow softkeys to select a deceleration profile, then ✮✷✽✮✻❛ press or another parameter key to continue. ✷✸❈✫✻✪✴✲✷✰❛...

  • Page 40: Activation Keys

    Run Preparation When the run ends, the hours and runs for the rotor in use are updated in the LAB ROTORS log. The ROTOR LOGGING field is cleared and the default NO ROTOR SELECTED is displayed until the next rotor entry is made. ACTIVATION KEYS NOTE ✹✻✲✷✽✮✻❛...

  • Page 41: More Options

    Run Preparation when the system is off.) When the run begins, the rotor will not accelerate past 3000 rpm until the chamber pressure drops below 750 microns. At that time, the rotor accelerates to set speed. ¤✹✻✮✬✸✸✵❛ The vacuum system is activated automatically when you select ✹✻✮✱✮✪✽❛...

  • Page 42: Programmed Runs

    Programmed Runs The Optima XL can store up to ten programs in the Program Library: nine standard (single-step) programs and one sequential program, which can have up to five steps. The programs are stored in memory (even with the power off) until called up by number for use or modification.
  • Page 43 Programmed Runs The softkey labels at the bottom of the screen change according to the PROGRAM LIBRARY function in process. CREATING OR MODIFYING A STANDARD PROGRAM To create or modify a standard program, do the following. ✹✻✸✰✻✪✶❈✵✲✫✻✪✻❂❛ 1. Press ✮✷✽✮✻❛ 2.

  • Page 44: Sequential Program

    Programmed Runs ✹✻✸✰✻✪✶❈✵✲✫✻✪✻❂❛ 1. Press the softkey. ✮✷✽✮✻❛ 2. Use the keypad to select a program by number and press . You will be returned to the Main Screen, with the selected program settings displayed in the SETTINGS window (Figure 3-2). 3.

  • Page 45: Figure 3-3. Sequential Program Windows

    Programmed Runs SEQUENTIAL PROGRAM STEPS SETTINGS–SEQ. PROGRAM: STEP A TIME/ SPEED STEP SPEED TEMP ACCEL DECEL (RPM) TIME REMAINING (HR:MIN) TEMP MODIFYING SEQ. PROG. STEPS A–E (DEG C) 00 RPM 0:00 H:M ACCEL: 26.1 DEG DECEL: P R E S S A S E T T I N G S K E Y T O E N T E R O R M O D I F Y T H I S S T E P ' S S E T T I N G S . P R E S S <...

  • Page 46: Figure 3-4. Entering Settings For Step A

    Programmed Runs SETTINGS–SEQ. PROGRAM: STEP A SEQUENTIAL PROGRAM STEPS TIME/ SPEED STEP SPEED TEMP ACCEL DECEL (RPM) TIME REMAINING (HR:MIN) TEMP MODIFYING SEQ. PROG. STEPS A–E (DEG C) 00 RPM 0:00 H:M ACCEL: 26.1 DEG DECEL: P R E S S < S A V E P R O G R A M > T O S A V E P R O G R A M S T E P S . ACCEL DECEL HOLD...
  • Page 47 Programmed Runs MODIFYING STEPS IN A SEQUENTIAL PROGRAM The steps in a sequential program can be modified or deleted as follows. (Run settings for ESP sequential programs cannot be changed.) 1. If necessary, press to clear program settings from the Main Screen before proceeding.

  • Page 48: Rotor Functions

    NOTE Although rotor logging and deration are no longer required for warranty purposes, Beckman Coulter recommends that you continue to log rotor usage, as this information is helpful to Field Service technicians if rotor repair is necessary. Also, your laboratory standard operating procedures may require rotor logging and deration.
  • Page 49 Displays specifications of the rotor selected for logging. (This softkey is not available if no rotor is selected.) ✶✸✭✲✯❂❈✲✷✯✸❛ Allows you to modify rotor information. ✻✸✽✸✻❈✬✪✽✪✵✸✰❛ Allows you to access the list of Beckman Coulter rotors and rotor specifications. ✶✪✲✷❈✼✬✻✮✮✷❛ Returns you to the Main Screen. ADDING A ROTOR Add a rotor to your LAB ROTORS log as follows: ✪✭✭❈✻✸✽✸✻❛...

  • Page 50: Figure 4-1. Adding A Rotor To The Lab Rotors Log

    Rotor Functions LAB ROTORS ROTOR CATALOG Ty p e 10 0T i Ty p e 30 VTI 65 ROTOR TOTAL Ty p e 9 0T i Ty p e 28 VTi 6 5.1 NO./MODEL RUNS HOURS Ty p e 8 0T i Ty p e 25 VTi 6 5.2 NO ROTOR LOGGING...

  • Page 51: Figure 4-2. Saving The Rotor Information In The Lab Rotors Log

    Rotor Functions LAB ROTORS SETTINGS ROTOR TOTAL NO./MODEL RUNS HOURS SPEED NO ROTOR LOGGING (RPM) TYPE 65 TIME REMAINING (HR:MIN) TEMP (DEG C) ADDING ROTOR INFORMATION 00 RPM @NEEDS DERATING ACCEL: ROTOR 0:00 H:M DECEL: •DERATED ROTOR LOGGING: 26.1 DEG U S E S O F T K E Y S T O E N T E R O R M O D I F Y A R O T O R N O .

  • Page 52: Selecting A Rotor For Logging

    Rotor Functions 6. Use the keypad to enter or modify the total number of hours that the rotor ✮✷✽✮✻❛ has run (in this and any other ultracentrifuge). Press You can use each OTHER entry only once. If you try to list it a second time, an error message will indicate that this entry has already been selected.

  • Page 53: Rotor Catalog And Rotor Specifications

    EXIT—NO SAVE ROTOR CATALOG AND ROTOR SPECIFICATIONS The ROTOR CATALOG window lists all available Beckman Coulter ultra- centrifuge rotors at the time of the Optima XL software release. New versions of the software, which include updated catalogs of available rotors, are released periodically.

  • Page 54: Figure 4-3. The Rotor Catalog And Rotor Specifications

    Rotor Functions ✻✸✽✸✻❛ Press while you are at the Main Screen to access the LAB ROTORS ✻✸✽✸✻❈✬✪✽✪✵✸✰❛ window. Then press the softkey to access the ROTOR CATALOG window, which appears on the right side of the screen (see Figure 4-3). ROTOR CATALOG ROTOR SPECIFICATIONS Ty p e 10 0T i...

  • Page 55: Figure 4-4. Selecting A Tube For Rotor And Tube Specifications

    Rotor Functions NOTE The full-size tube is always given as first in the list and is not a g-Max tube. 3. When a specific tube is highlighted, the specifications for that rotor/tube combination appear in the window on the left. Figure 4-4 shows the specifications for the Type 90 Ti rotor with a 6.5 mL tube.

  • Page 56: The Printer

    The Printer This section describes use of the built-in printer that provides a variety of instrument and run data printouts. See Section 9 for information on the care of the printer, including changing the paper and ribbon. PRINTER MENU WINDOW ✹✻✲✷✽✮✻❛...

  • Page 57: Menu Options

    The Printer STATUS INFORMATION Status information will be displayed at the top of the window as follows, depending on the selections made from the printer menu. • XL Usage Log—Indicates whether instrument usage logging is ON or OFF. Be sure to have this ON if you intend to print out the usage records at a later time.

  • Page 58: Figure 5-2. Sample Printout Of Instrument Usage

    The Printer PRINT INSTRUMENT USAGE AND PRINT ROTOR USAGE These are two separate logs, each with the capacity to store 40 records. The maximum length of a “page” for either printout is eight inches. If the list of records is more than a page long, subsequent pages are printed as required. NOTE It is important to print out each log regularly since printing clears the records for reuse.

  • Page 59: Figure 5-3. Sample Printout Of Rotor Usage

    The Printer Figure 5-3. Sample Printout of Rotor Usage Each Rotor Usage Record includes the rotor line number (from the list of lab rotors) and model name, maximum rotor speed, rotor serial number, total number of runs, and total hours run. (A run is considered valid if the rotor accelerated beyond 3000 rpm.) A line is provided at the end of the record for the signature of the operator who updates the records.

  • Page 60: Figure 5-4. Sample Printout Of Current Settings

    The Printer Figure 5-4. Sample Printout of Current Settings Figure 5-5. Sample Printout of Current Run Results...
  • Page 61 The Printer A line beneath the tube size indicates the manner in which the run was terminated. • Run completed. • Run stopped by operator. • Run stopped by diagnostic xx (actual diagnostic number is provided). At the end of the record there are places for the operator to sign the record and provide the run number.
  • Page 62 The Printer SET TIME ✮✷✽✮✻❛ Select Set Time and press to enter or modify the time shown on the printouts. The time is shown as hours:minutes (hh:mm), using the 24-hour format. ✵✮✯✽❛ ✻✲✰✱✽❛ Use the arrow softkeys to position the cursor on the digit to be changed.

  • Page 63: Calculations

    Calculations 1.5 g/mL ρ rpm = (90 000) The Calculations feature performs a variety of calculations commonly used in ultracentrifugation. These calculations, which may be used while the instru- ment is at rest or while a run is in progress, are provided to help simplify your run preparation.

  • Page 64: Entering Information

    Calculations ENTERING INFORMATION Note the following general points on entering information before proceeding with individual calculations. The prompt lines and softkey labels will change as required to assist you in executing the calculations. • Use the arrow softkeys or the keypad to highlight the required calculation ✮✷✽✮✻❛¤...
  • Page 65 Calculations CONVERT RPM – – – CONVERTS BETWEEN SPEED (RPM) AND RELATIVE CENTRIFUGAL FORCE (RCF) R O TOR TUBE r A V r M A X ENTER A VALUE IN ANY ONE OF THE FIELDS BELOW SPEED – –– RCFav RCFmax P R E S S T H E R O T O R S O F T K E Y F O R R O T O R O P T I O N S .

  • Page 66: Calculate Pelleting Time

    Calculations RCFav—RCFav is calculated if you entered a value for either RCFmax or speed. RCFmax—RCFmax is calculated if you entered a value for either RCFav or speed. CALCULATE PELLETING TIME To select this calculation (Figure 6-3), use the arrow keys to highlight it, then ✮✷✽✮✻❛...

  • Page 67: Concentration Conversions

    Calculations rMAX—The radial distance (in millimeters) from the rotor’s axis to the furthermost surface of the tube is filled in automatically when you select a rotor from the catalog. You may enter a different value manually, then press ✮✷✽✮✻❛ . If you change rMAX, however, the ROTOR and TUBE fields will be cleared, but the calculation may still be executed.
  • Page 68 Calculations CONCENTRATION CONVERSIONS FOR SUCROSE AND CsCl CONVERTS BETWEEN %WT/WT, %WT/VOL, AND MOLARITY GRADIENT SOLUTE = CsCl @20 DEG C ENTER ONE OF THE FOLLOWING: D E NS ITY g / mL MO LA R IT Y %W T/VOL %W T/W T U S E K E Y P A D T O E N T E R O R M O D I F Y D E N S I T Y .

  • Page 69: Refractive Index Conversions

    Calculations REFRACTIVE INDEX CONVERSIONS To select this calculation (Figure 6-5), use the arrow keys to highlight it, then ✮✷✽✮✻❛ press DENSITY/REFRACTIVE INDEX CONVERSIONS CALCULATES VALUES OF DENSITY, REFRACTIVE INDEX, OR CONCENTRATIONS GRADIENT SOLUTE = CsCl @20 DEG C ENTER ONE OF THE FOLLOWING: R EFR AC TIVE INDEX g/ mL DE NSITY...

  • Page 70: Convert Rpm, Time, And W2T

    Calculations CONVERT RPM, TIME, AND W2T To select this calculation (Figure 6-6), use the arrow keys to highlight it, then . ( ω ✮✷✽✮✻❛ press t is represented as W2T on the screen.) CONVERT RPM –– – TIME – – – CONVERTS BETWEEN VALUES OF SPEED, TIME, AND W2T ENTER TWO OF THE FOLLOWING: SPEED...

  • Page 71: Speed Reduction, Dense Solutions

    Calculations SPEED REDUCTION, DENSE SOLUTIONS The maximum speed permitted for ultracentrifuge rotors must be limited to prevent stress on the rotor as well as to prevent precipitation of gradient materials such as cesium chloride. Where precipitating salts are used, the lower of these speed limits should be selected.

  • Page 72: Speed Reduction, Precipitating Solutions

    Calculations MAX SPEED—The maximum speed permitted is entered automatically when you select a rotor/tube combination from the catalog. You may change this value, but in doing so you will clear the ROTOR/TUBE fields. To change ✮✷✽✮✻❛ this value, use the keypad, then press AVERAGE DENSITY OF TUBE CONTENTS—Use the keypad to enter a ✮✷✽✮✻❛...

  • Page 73: Run Duplication

    Calculations TUBE—Use the arrow softkeys to select a g-Max tube for the calculation, ✮✷✽✮✻❛ then press MAX SPEED—The maximum speed permitted is entered automatically when you select a rotor/tube combination from the catalog. You may change this value, but in doing so you will clear the ROTOR/TUBE fields. To change ✮✷✽✮✻❛...
  • Page 74 Calculations USER INPUTS After entering the following information for ROTOR 1, enter the inputs for ROTOR 2. ✻✸✽✸✻❛ ROTOR—Press the softkey (or hardkey) to access the ROTOR ✮✷✽✮✻❛ CATALOG. Use the arrow softkeys to highlight ROTOR 1. Press TUBE—Use the arrow softkeys to select a g-Max tube for this rotor and ✮✷✽✮✻❛...

  • Page 75: Esp Efficient Sedimentation Program

    ™ Efficient Sedimentation Program Use of the Efficient Sedimentation Program is described here. THE EFFICIENT SEDIMENTATION PROGRAM To provide the shortest possible run times in precipitating media without the risk of damage to the rotor or centrifuge, the Optima XL provides an ESP™ (Efficient Sedimentation Program), which simulates the formation of the gradient and separation of sample components in a selection of Beckman Coulter ultracentrifuge rotor/tube combinations.

  • Page 76: The Softkeys

    ESP™ Efficient Sedimentation Program optimized for speed of separation. The sample is suspended in buffer containing 4 GuSCN without CsCl, and is layered over a CsCl cushion occupying one-fourth the volume of the tube. To prevent large DNA from pelleting along with the RNA in this separation, the cell or tissue lysate must be sheared to provide DNA fragments smaller than 2 kbp.

  • Page 77: Entering Information

    ESP™ Efficient Sedimentation Program ENTERING INFORMATION ✮✷✽✮✻❛ • Use the arrow softkeys to highlight the menu item and press . Or ✮✷✽✮✻❛ use the keypad to enter numeric values, when necessary, and press ✮✷✽✮✻❛ In either case, pressing moves you to the next field needing input. ✹✻✮✿✲✸✾✼❈✯✲✮✵✭❛...

  • Page 78: The Esp Protocol Definition Screen

    ESP™ Efficient Sedimentation Program THE ESP PROTOCOL DEFINITION SCREEN 1. You may enter a default value (cursor in Figure 7-3 is on the screen update ✮✷✽✮✻❛ field) for the size of the DNA or RNA by pressing or you may enter another value into the highlighted component size field by using the ✮✷✽✮✻❛...
  • Page 79 ESP™ Efficient Sedimentation Program EFFICIENT SEDIMENTATION PROGRAM ROTOR CATALOG RNA Pelleting in CsCl with GuSCN, Optimized for Speed T yp e 1 00T i Ty p e 3 0 VTI 6 5 Component T yp e 9 0T i Ty p e 2 8 VT i 65.1 1.00 (R NA)

  • Page 80: Simulate The Run

    ESP™ Efficient Sedimentation Program SIMULATE THE RUN ✼✲✶✾✵✪✽✮❈✻✾✷❛ Press to display a graphic representation of the run (see Figure 7-6). The simulation is represented by a number of graphical data sets. A message on the screen indicates which data set is being calculated. As each data set is shown, the values at the left are updated.

  • Page 81: Run Esp Protocol

    ESP™ Efficient Sedimentation Program ESP EFFICIENT SEDIMENTATION PROGRAM ROTOR NAME TYPE 100 Ti 1.92 SPEED 63200 RPM TUBE VOLUME 5.60 mL (actual) ELASPED TIME 6.30 HR:MIN 1.69 DATA SET NO. TOTAL ELAPSED 1.46 TIME 6.54 HR:MIN Bottom curves show relative 1.23 concentration of components.
  • Page 82 ESP™ Efficient Sedimentation Program CURRENT VALUES SETTINGS–ESP PROGRAMMED RUN SPEED SPEED (RPM) (RPM) TIME TIME REMAINING (HR:MIN) (HR:MIN) TEMP TEMP (DEG C) (DEG C) VACUUM: OFF ACCEL: ROTOR DECEL: LOGGING: TYPE 100 Ti Figure 7-8. Using the ESP Settings Once selected, the ESP settings usually cannot be changed. However, after the ESP run settings are entered and you return to the Main Screen, you can select a DECEL profile for the last step of the run as follows.

  • Page 83: Summary Of Run Procedures

    The Optima XL is designed for normal, locked, and zonal operation. Use any Beckman Coulter rotor and consult the applicable rotor manual for detailed information on preparing the rotor for centrifugation. Each rotor manual includes rotor specifications, information on loading/unloading...

  • Page 84: Key In The Normal Position

    Summary of Run Procedures KEY IN THE NORMAL POSITION STANDARD OPERATION 1. Turn the key to the right (clockwise) to the normal position. 2. Enter the run settings—SPEED, TEMP, and TIME, HOLD, or ω t— following the prompts on the screen. 3.

  • Page 85: Key In The Locked Position

    Summary of Run Procedures ✼✽✸✹❛ Press to terminate a run in the HOLD mode. Runs in the TIME and ω t modes will terminate automatically. After the rotor has stopped, press ✿✪✬✾✾✶❛ to vent the chamber. ✼✽✸✹❛ is pressed during an ESP or sequential run, the run will be termi- ✮✷✽✮✻❛...

  • Page 86: Key In The Zonal Position

    Summary of Run Procedures KEY IN THE ZONAL POSITION WARNING In zonal operation, the operator is unavoidably exposed to rotating machinery. For safety, the operator must be properly instructed and qualified. Guard against accidentally dropping objects into the chamber. Loose lab coats, neckties, scarves, and long necklaces should not be worn while operating in the zonal mode.
  • Page 87 Summary of Run Procedures NOTE If you are performing consecutive zonal runs: at the end of each zonal run, the centrifuge will automatically reset to the normal mode. To reconfirm selection of the zonal mode, you must turn the key left to right (clockwise) from the zonal position to the normal position and then ✮✷✽✮✻❛...

  • Page 88: Troubleshooting And Maintenance

    This section contains troubleshooting and maintenance procedures that should be performed regularly. Troubleshooting and maintenance not covered in this manual should be handled by your Beckman Coulter Field Service representative. Refer to the applicable rotor manual and Chemical Resistances (publication IN-175) for instructions on the care of rotors and their accessories.

  • Page 89: Retrieving Your Sample

    Troubleshooting and Maintenance RETRIEVING YOUR SAMPLE WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of elec- trical shock and/or physical injury. Therefore, turn the power OFF and disconnect the instrument from the main power source, and refer such maintenance to service personnel.
  • Page 90 Troubleshooting and Maintenance Access to Cover Latch Cover Latch Remove Key Figure 9-1. Removing the Control Head Cover Top Panel Tabs Front Panel Access Port Front Panel Tabs Figure 9-2. Depressing the Latch to Loosen the Front Panel...
  • Page 91 Troubleshooting and Maintenance WARNING After removing the panel, listen carefully for any sounds coming from the drive. Then touch the fan housing (see Figure 9-3) to feel if it is vibrating and listen again. Do not proceed if any sound or vibration is emitted from the housing.

  • Page 92: Error Messages

    Troubleshooting and Maintenance 8. After retrieving the sample, close the chamber door, then replace the front panel on the instrument. Insert the tabs at the bottom of the panel onto the lip at the base of the instrument, being sure to align the front panel with the side panels.

  • Page 93: Diagnostic Messages

    If necessary, the instrument will automatically trip the power. You will be prompted to take corrective action or, if there is no operator solution, call your Beckman Coulter Field Service representative. Each diagnostic category has a range of numbers (and letters if necessary) to assist Field Service in determining the nature of the problem.

  • Page 94: Cleaning

    1x or 9x program memory service. If trouble persists, call Beckman Coulter Field Service. (1-800-551-1150 in the United States; worldwide offices are listed at the back of this manual). CLEANING Clean instrument surfaces using a cloth dampened with a mild detergent solution such as Beckman Solution 555™...

  • Page 95: Decontamination

    Refer to Chemical Resistances (publication IN-175) or contact Beckman Coulter Field Service to ensure that the decontamination method does not damage any part of the instrument (or...

  • Page 96: Sterilization And Disinfection

    See Chemical Resistances for more information regarding chemical resistance of instrument and accessory materials. While Beckman Coulter has tested these methods and found that they do not damage the instrument, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your labo- ratory safety officer regarding proper methods to use.

  • Page 97: Printer Maintenance

    Troubleshooting and Maintenance PRINTER MAINTENANCE To change the ribbon (927506) or replace the paper (927505), you must remove the control head cover to gain access to the printer. Follow the instruc- tions provided under Retrieving Your Sample earlier in this section. Once the control head cover is removed, locate and review the summary of instructions fastened to the inside of the cover.
  • Page 98 Troubleshooting and Maintenance 3. Lift the grooved rod from the slots to remove the old paper roll. Discard the old paper roll but keep the rod. 4. Trim the edge of the new paper at an angle, with the pointed edge on the right.
  • Page 99 Troubleshooting and Maintenance Paper Guide and Cutter Latch Figure 9-8. Removing the Paper Guide and Plastic Cutter 2. Pull the latch to the left, lift the printer assembly up, and tilt it toward you. Release the latch so that it holds the printer in the raised position. 3.
  • Page 100 Troubleshooting and Maintenance Lever Ribbon Guide Driving Pins Empty Spool Figure 9-9. Inserting the New Ribbon Figure 9-10. Ribbon Spools Installed 9-13...

  • Page 101: Supply List

    1050 Page Mill Road, Palo Alto, CA 94304, U.S.A. (Telephone 650-859-1753; Fax 650-859-1375). Call Beckman Coulter Sales (1-800-742-2345 in the United States; world- wide offices are listed at the back of this manual) or see Ultracentrifuge Rotors, Tubes & Accessories (BR-8101) for detailed information on ordering parts and supplies.
  • Page 102 Beckman Coulter personnel. portation charges collect unless the product is found to be defective, in which case Beckman Coulter will pay all trans- 4. The drive unit was installed by a Beckman Coulter Field portation charges. Service representative.
  • Page 103 Republic of South Africa Telephone: (27) 11-805-2014/5 Fax: (27) 11-805-4120 Beckman Coulter, Inc. • 4300 N. Harbor Boulevard, Box 3100 • Fullerton, California 92834-3100 Sales: 1-800-742-2345 • Service: 1-800-551-1150 • Internet: www.beckmancoulter.com • Telex: 678413 • Fax: 1-800-643-4366 ©2001 Beckman Coulter, Inc.

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