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Functional Pages
ESP Pelleting Separation Page
• When you have entered the fields, select the
simulation is graphically displayed.
— The blue curve represents the relative concentration of the RNA.
— The green curve represents the relative concentration of the DNA contaminant.
— The red curve represents the density of the CsCl gradient.
— The dotted line at the top of the graph represents the concentration at which CsCl
precipitates.
The maximum time required for the separation is shown above the slide bar. Use the slide bar
to display separations at shorter run times. The y-axis represents both the relative
concentration of the particles and the density of the CsCl gradient during centrifugation. The
x-axis indicates the position of the particles along the length of the tube with the bottom of the
tube at the right.
• The
Reset
• The
Back
• The
Transfer
a live run.
• The
Save
a later time.
NOTE
Saving or transferring the simulated run settings does not save the simulation inputs, only the
current simulated run settings (including the adjustments for a shorter elapsed time, if applicable).
ESP Pelleting Separation Page
Figure 8.80 ESP Pelleting Separation Page
8-76
button clears the
,
Rotor
button returns to the
Simulations
button transfers the current simulated run settings to the instrument settings for
button saves the simulated run settings as a named run program, which can be run at
button to display the simulated run. The
Simulate
and Molecular Length fields.
Labware
Page.
PN B08115AF
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