Esp Optimized Plasmid Dna Separation Page - Beckman Coulter Optima XPN User Manual

Simulations
Using Simulations
Figure 7.7 ESP Optimized Plasmid DNA Separation Page
2
Select
Rotor
3
Select the button with the blue indicator to enter the value for molecular weight of DNA, then
select
OK
The same value of molecular weight entered for supercoiled DNA appears in the button with the
green indicator, representing the linear (nicked) DNA of the same molecular weight.
4
Select
Simulate
The following describes the graph:
• The green curve represents the relative concentration of linear DNA.
• The blue curve represents the relative concentration of supercoiled DNA.
• The red curve represents the density of the CsCl gradient along the tube.
• The dashed line at the top of the graph represents the concentration at which CsCl
precipitates.
• The dashed line near the middle of the graph represents the initial density (1.55 g/mL) of
the gradient.
Move the slider to the left to display separations at a shorter run time.
7-10
or to select a rotor and labware combination, then select
Labware
.
. The simulation is displayed on the graph.
.
OK
PN B08115AF