Esp Rna Pelleting Best Run; Simulations Page - Beckman Coulter Optima XPN User Manual

Figure 7.2 Simulations Page

ESP RNA Pelleting Best Run

This function simulates separation of RNA molecules in the range 0.1 to 3.0 kb from the
chromosomal DNA through a cushion of 5.7 M CsCl at 25°C in a swinging bucket rotor. In the
simulation, the sample suspended in 2.91 M Cesium Chloride (CsCl) containing 4 M Guanidine
Thiocyanate (GuSCN) layered over a CsCl cushion occupying one-third of the tube volume. The
simulation is optimized for purity.
To simulate an ESP RNA pelleting run optimized for purity:
1
On the
The system displays the ESP RNA Pelleting in CsCI with GuSCN, Optimized for Purity page.
PN B08115AF
page, select
Simulations ESP RNA Pelleting Best Run
Simulations
Using Simulations
.
7
7-3