Esp Rna Pelleting Fast Run; Esp Rna Pelleting In Csci With Guscn, Optimized For Speed - Beckman Coulter Optima XPN User Manual

Preparative ultracentrifuges
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6
Select
Transfer
live run. Select
can be run at a later time.

ESP RNA Pelleting Fast Run

This function simulates separation of RNA molecules in the range 0.1 to 3.0 kbp from the sheared
chromosomal DNA molecules through a cushion of 5.7 M CsCl at 25°C in a swinging bucket rotor. In
the simulation, the sample suspended in 4 M GuSCN and layered over a CsCl cushion occupying one-
fourth of the tube volume. This simulation is optimized for speed.
To simulate an ESP RNA pelleting run optimized for speed:
1
On the
The system displays the ESP RNA Pelleting in CsCI with GuSCN, Optimized for Speed page.
Figure 7.4 ESP RNA Pelleting in CsCI with GuSCN, Optimized for Speed Page
2
Select
Rotor
NOTE
For this simulation, only swinging bucket rotors are available.
PN B08115AF
to transfer the current simulated run settings to the instrument settings for a
to save the current simulated run settings as a named run program, which
Save
page, select
Simulations
ESP RNA Pelleting Fast Run
or
to select a rotor and labware combination, then select
Labware
Simulations
Using Simulations
.
.
OK
7
7-5

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