Set The 2D-Lc Method Parameters; 2D Lc Operation Mode - Agilent Technologies 1290 Infinity II 2D-LC User Manual

6 Method Parameters

Set the 2D-LC Method parameters

Set the 2D-LC Method parameters

2D LC Operation Mode

Setting the mode has the following consequences:
Heart-Cutting (LC-LC)
The Heart-Cutting mode covers two 2D-LC applications Heart Cutting (LC-LC)
and High-Resolution Sampling (HiRes). Once you have selected the Heart-Cutting
mode, you can later define in the software whether you want to use one or the
other mode or even both together.
In Heart-Cutting modus, a relevant volume of
2
D column using the
volume to be injected on the
with the peak of interest. Then the injection on the
of the
For details Setting this mode, see
In contrast to Heart-Cutting, which uses the continuous flowthrough principle, in
high-resolution sampling (HiRes) the Multiple Heart-Cutting (MHC) valve is
switched before and after parking the peak.
When setting up the experiments, keep the following general considerations in
mind:
•
•
•
•
•
For details Setting this mode, see
Principles"
2D-LC User Guide
2
D pump. A peak trigger or a time window defines the
2
D pump.
Each loop for consecutive snips stores the same sample volume.
First and last loop cannot be used for parking.
Solvent transfer from
Cut number 5 cannot be parked entirely in the Sample Loop. Otherwise cut 6
would got partially to the transfer capillary and would therefore be lost or spoil
cut 5. Cut 5 stays partially in the transfer line and is immediately being
2
analyzed in D.
For parking cut 6 in the Sample Loop, the cut first needs to be moved from the
2D-LC Valve to the deck valve. This new volume must be defined in the
configuration of the 2D-LC system.
on page 25.
1
2
D column. When heart-cutting starts, a loop is filled
"Heart-Cutting 2D-LC (LC-LC)"
1 2
D to D can be reduced.
"High Resolution Sampling - Peak Parking
D is cut off and injected onto the
2
D starts running the gradient
on page 18.
127