Orthogonality - Agilent Technologies 1290 Infinity II 2D-LC User Manual

14 Theoretical Background
Theoretical basis of 2D-LC
The following different methods of 2D-LC exist:
•
•

Orthogonality

The 2D-LC separation power depends the fact that the two selectivity
mechanisms in the different separation stages must be as different as possible.
If the mechanisms are completely different and independent the two separations
are called orthogonal.
Any correlation between the selectivity mechanisms degrades orthogonality and
reduces the efficiency of the 2D-LC system.
For strategies to achieve maximum orthogonality, refer to
and
2D-LC User Guide
Heartcutting (LC-LC)
Only interesting portion of the first dimension effluent transferred to the
second dimension.
Comprehensive (LCxLC)
Entirety of first dimension effluent sequentially transferred to the second
dimension.
Table 46 on page 378.
Table 45 on page 373
364