Prepare The Experiment - Agilent Technologies 1290 Infinity II 2D-LC User Manual

8 Run the System

Prepare the Experiment

Prepare the Experiment

Parts required p/n
5190-6895
G2453-85060
858700-902
857768-901
959757-302
Hardware required Various hardware configurations are possible, see
Preparations Take care that the following solvents for mobile phases are available:
•
•
Preparation of 4 mL sample (1:100) for LCMS
1 To prepare dilution solvent, add 800 µL methanol to 3200 µL Mobile Phase A.
2 To prepare sample (1:100), add 40 µL 2D-LC starter sample to 3960 µL
Preparation of 4 mL sample (1:1000) for LCMS
1 To prepare dilution solvent, add 800 µL methanol to 3200 µL Mobile Phase A.
2 To prepare sample (1:1000), add 4 µL 2D-LC starter sample to 3996 µL
2D-LC User Guide
Description
2D-LC starter sample, 1 x 2 mL
Formic Acid-Reagent Grade 5 mL (5 cc)
RRHD SB-C18, 2.1x100 mm, 1.8 µm, 1200 bar
1
In D
RRHD Bonus-RP, 2.1x50 mm, 1.8 µm, 1200 bar
2
In D for Heart Cutting (LC-LC) and High Resolution (High Res)
RRHD Eclipse Plus C18, 3.0x50 mm, 1.8 µm
2
In D for Comprehensive 2D-LC (LCXLC)
1D:
• A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060)
• B = methanol
2D:
• A = water with 0.2 % Formic Acid-Reagent Grade 5 mL ( 5 cc) (G2453-85060)
• B = acetonitrile
4000 µL dilution solvent (20 % methanol in mobile phase A) is prepared.
dilution solvent.
4 mL sample (1:100) is prepared.
A further dilution step to 1:1000 may be useful.
4000 µL dilution solvent (20 % methanol in mobile phase A) is prepared.
dilution solvent.
4 mL sample (1:1000) is prepared.
"Options" on page 55.
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