Comprehensive 2D-Lc (Lcxlc) - Agilent Technologies 1290 Infinity II 2D-LC User Manual

2 Concepts of 2D-LC

Comprehensive 2D-LC (LCxLC)

Comprehensive 2D-LC (LCxLC)

In comprehensive 2D-LC (also known as LC×LC), the total eluent from the first
dimension is injected on to the column in the second dimension using two
equal-sized sampling loops that are alternated by a switching valve. While the
first loop is being filled in the first dimension, the contents of the second loop is
analyzed in the second dimension; the switching valve then switches the second
loop into the first dimension for sampling and the first loop into the second
dimension for analysis.
The gradient analysis in the second dimension is less than or equal to the cut
size time in the first dimension:
%B
%B
Figure 7
2D-LC User Guide
2
(
After t D) return to %B
stop
0
2
(
D)
t
stop
t
Characteristics of LCxLC
0
mod
Valve switches
Repeat n -times
mod
30