Run The Checkout Familiarization Procedure For High Resolution (Lc-Lc) - Agilent Technologies 1290 Infinity II 2D-LC User Manual

8 Run the System
Run the Experiment
Run the Checkout Familiarization Procedure for High
Resolution (LC-LC)
To run the checkout, various hardware configurations are possible, see
on page 56. Not all options can be shown. As example the
is used here.
The following parameters have been optimized for this standard configuration.
Parameters can deviate slightly for your system. Run the experiment and
subsequently edit or optimize the method for your setup.
Table 21
Parameter
Column
Column temperature
Stop time
Mobile Phase A
Mobile Phase B
Flow Rate
Post time
Mobile Phase
Gradient:
Injection Volume
Injection Needle Wash In Flush Port, 10 s, acetonitrile/water (50/50)
Stop time
Diode-array Detector
Signal A
Reference Wavelength 360 nm
Reference Bandwidth
Peak width
Stop time
2D-LC User Guide
Recommended conditions in 1D (HPLC) for SHC, MHC, and HiRes 2D-LC
Value
1
D Column Compartment (MCT)
RRHD SB-C18, 2.1x 100 mm, 1.8 µm, 1200 bar (858700-902)
40 °C
As pump/No limit
1
D Pump
Water + 0.2 % formic acid
Methanol
0.6 mL/min
6 min
20 % B 0.00 min
100 % B 50 min
Autosampler
2 µLfor Standard LC
0.5 µL Positive Mode for LCMS
10 µL Negative Mode for LCMS
As pump/No limit
1
D Detector (DAD)
254 nm, Bandwidth 4 nm
100 nm
20 Hz
Stop time As pump/No limit
Table 5
Table 14 on page 198
210