Optimize The Dilution By Using Asm Capillaries - Agilent Technologies 1290 Infinity II 2D-LC User Manual

7 Method Development of Active Solvent Modulation (ASM)

Optimize the Dilution by Using ASM Capillaries

Optimize the Dilution by Using ASM Capillaries

A choice of four different ASM capillaries is available for achieving best results.
Longer capillaries reduce, shorter capillaries increase the dilution of 1D solvent in
the sample loop. Install and configure different ASM capillaries (see "Configure
the ASM Valve" on page 190) for optimizing the results.
Capillary p/n Length (mm)
5500-1300 85
5500-1301 170
5500-1302 340
5500-1303 680
2D-LC User Guide
Inner diameter Volume
(mm) (µl)
0.12 0.96
0.12 1.9
0.12 3.8
0.12 7.7
ASM factor Split ratio
(loop:ASM)
5 1:4
3 1:2
2 1:1
1.5 1:0.5
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