Use The Flush Gradient - Agilent Technologies 1290 Infinity II 2D-LC User Manual

6 Method Parameters
Set the 2D-LC Method parameters

Use the Flush Gradient

The Flush Gradient can be used to flush the transfer capillaries and Sample
Loops. You can choose to use the analytical gradient or you can set up a custom
flush gradient. If you choose the Customize flush gradient option, specify a
gradient Duration. The Equilibration time is the same as that set in the Gradient
Phase section.
1 To use the analytical gradient as flush gradient, select the radio button Use
Flush Gradient is only be used in heart cutting mode. If you have selected
NOTE
comprehensive, this feature is unavailable.
Setup Flush Gradient can also be done graphically in the preview. The Flush
NOTE
Gradient is displayed in orange.
2D-LC User Guide
analytical gradient as flush gradient.
OR
To customize a flush gradient, select the radio button Customize flush gradient
and use the table to set up the custom gradient:
a Specify the duration time in the Analysis duration field.
The equilibration value is read only and is defined in the Gradient Phase
settings, see "Specify the Gradient Phase"
b Specify the time for the change of solvent composition in the Time [min]
field.
c Specify the percentage of solvent that channel B delivers at the specified
time.
Channel A always delivers the remaining volume, %A = (100 - %B). The
solvent composition changes linearly from one setpoint to the next.
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